Comparison of cellular damage response to low-dose-rate 125I seed irradiation and high-dose-rate gamma irradiation in human lung cancer cells

Chen, Honghong; Bao, Yuanyuan; Yu, Lei; Jia, Rongfei; Wenying, Cheng; Shao, Chunlin

doi:10.1016/j.brachy.2011.05.002

Cited by 14 publications

(12 citation statements)

References 20 publications

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“…In mammals, most DSBs are repaired by nonhomologous end joining [44,50,56]. Protein KU70 is essential for this process and is induced in mammalian cells exposed to X-rays [57], gamma-rays [58], as well as UV radiation [42]. We note a significant upregulation of KU70 in THz and UVA-exposed tissues, as shown in Fig.…”

Section: Resultsmentioning

confidence: 72%

Intense THz pulses cause H2AX phosphorylation and activate DNA damage response in human skin tissue

Titova

Ayesheshim

Golubov

et al. 2013

Biomed. Opt. Express

132

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Recent emergence and growing use of terahertz (THz) radiation for medical imaging and public security screening raise questions on reasonable levels of exposure and health consequences of this form of electromagnetic radiation. In particular, picosecond-duration THz pulses have shown promise for novel diagnostic imaging techniques. However, the effects of THz pulses on human cells and tissues thus far remain largely unknown. We report on the investigation of the biological effects of pulsed THz radiation on artificial human skin tissues. We observe that exposure to intense THz pulses for ten minutes leads to a significant induction of H2AX phosphorylation, indicating that THz pulse irradiation may cause DNA damage in exposed skin tissue. At the same time, we find a THz-pulse-induced increase in the levels of several proteins responsible for cell-cycle regulation and tumor suppression, suggesting that DNA damage repair mechanisms are quickly activated. Furthermore, we find that the cellular response to pulsed THz radiation is significantly different from that induced by exposure to UVA (400 nm).

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Section: Resultsmentioning

confidence: 72%

Intense THz pulses cause H2AX phosphorylation and activate DNA damage response in human skin tissue

Titova

Ayesheshim

Golubov

et al. 2013

Biomed. Opt. Express

132

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“…Therefore, blocking or reducing the binding of Ku to broken DNA strands can influence the radiation sensitivity of cancer cells [17][18][19][20]. Chen et al indicated that Ku80 expression was negatively correlated with radiation sensitivity in low dosage radiotherapy [21], and Ku protein expression could be used for predicting the radiation sensitivity of cells [22][23][24][25][26]. In tumour radiotherapy, cancers can be treated via energy accumulation that will cause DNA DSB in cells.…”

Section: Discussionmentioning

confidence: 99%

AgNPs and Ag/C225 Exert Anticancerous Effects via Cell Cycle Regulation and Cytotoxicity Enhancement

Zhang

Lü

et al. 2017

Journal of Nanomaterials

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Currently, cancer has become the leading cause of deaths and is a serious threat to human health. Nanotechnology-based medical applications are developing rapidly, especially in anticancer research, as nanomaterials can be retained in the cell after their entry across the cell membrane, making them potential candidates for application in the diagnosis and treatment of tumours. Here, we prepared silver nanoparticles (AgNPs) and silver/cetuximab (Ag/C225) particles of approximately 20 nm and investigated their inhibitory effect on proliferation of human nasopharyngeal carcinoma cell line CNE and laryngeal carcinoma cell line HEp-2. The MTT assay was used to determine their half-maximal inhibitory concentration (IC50) values. Electron microscopy revealed no obvious morphological differences between the prepared AgNPs and Ag/C225. Treatment of both cell lines with AgNPs and Ag/C225 increased the proportion of cells in the G0/G1 stage and decreased the proportion of cells in the S stage. AgNP and Ag/C225 treatments increased the expression of apoptotic proteins Bax and P21 and decreased the expression of Bcl-2 in CNE cells, compared to control cells. Ag/C225 is internalised by CNE cells, and it enhances X-irradiation-induced cytotoxicity. These results help increase the understanding of the anticancer effect of AgNPs and Ag/C225 and explore their application as radiosensitisers.

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“…Previous studies have shown that the low radiation dose rate could increase the sensitivity of hypoxic tumor cells to radioactive rays and produce radiation-induced bystander effect to kill tumor cells [ 7 , 14 ]. In vitro studies have shown that 125 I radioactive seeds up-regulated apoptosis-related genes, and regulated cell cycle and apoptosis of tumor cells [ 15 ]. Taken together, 125 I radioactive seeds have the advantages including high conformality index and producing high dose in target volumes and low radioactive exposure to surrounding normal tissues, thus having great potential in the treatment of locally recurrent tumors [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

Combination of Iodine-125 brachytherapy and chemotherapy for locally recurrent stage III non-small cell lung cancer after concurrent chemoradiotherapy

Jin

Zhong

et al. 2015

BMC Cancer

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BackgroundLocally recurrent non-small cell lung cancer (NSCLC) poses a great challenge to physicians. This study aimed to explore the efficacy and safety of the combination of brachytherapy and docetaxel and cisplatin for the treatment of locally recurrent stage III NSCLC.MethodsFifty two patients with locally recurrent stage III NSCLC after concurrent chemoradiotherapy were randomly divided into two groups (n = 26). The patients in experimental group were treated with implantation of radioactive 125I seeds and DP regimen (docetaxel 60 mg/m2/cisplatin 75 mg/m2). Patients in control group received DP chemotherapy. The local control rate (LCR), progression-free survival (PFS), and overall response rate (ORR) were defined according to the Response Evaluation Criteria in Solid Tumors (RECIST).ResultsWith a median follow-up time of 11 months, PFS and LCR was 8 months (95 % CI: 6.99–9.01 months) vs. 5.5 months (95 % CI: 4.43–6.57 months) (P < 0.05) and 10 months (95 % CI: 8.72–11.28 months) vs. 6.2 months (95 % CI: 5.27–7.13 months) (P < 0.05) in the experimental and control groups, respectively. The ORR did not differ between treatment groups and was noted to be 69.2 % and 57.7 %, respectively (P >0.05). There was no occurrence of severe complications in experimental and control groups.ConclusionThe combination of 125I brachytherapy and second-line chemotherapy is superior to chemotherapy alone and is an effective and safe therapy for this disease.Trial registration numberChiCTR-IOR-15006560

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Comparison of cellular damage response to low-dose-rate 125I seed irradiation and high-dose-rate gamma irradiation in human lung cancer cells

Cited by 14 publications

References 20 publications

Intense THz pulses cause H2AX phosphorylation and activate DNA damage response in human skin tissue

Intense THz pulses cause H2AX phosphorylation and activate DNA damage response in human skin tissue

AgNPs and Ag/C225 Exert Anticancerous Effects via Cell Cycle Regulation and Cytotoxicity Enhancement

Combination of Iodine-125 brachytherapy and chemotherapy for locally recurrent stage III non-small cell lung cancer after concurrent chemoradiotherapy

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