Comparison of Cellular and Differentiation Characteristics of Mesenchymal Stem Cells Derived from Human Gingiva and Periodontal Ligament

Subba, Tarona Azem; Varma, Sudhir; Thomas, Biju; Rao, Shama; Kumar, Mohana; Talwar, Avaneendra; Shashidhar, Keerthan

doi:10.4103/jispcd.jispcd_259_21

Cited by 9 publications

(2 citation statements)

References 27 publications

(68 reference statements)

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“…Ten healthy periodontal premolars that needed to be extracted from orthodontic patients were collected at Beijing Stomatological Hospital after patient consent was obtained, and the procedure was approved by the Ethics Committee of Capital Medical University in China. Human PDLSCs were isolated and cultured following a previously described procedure (Subba et al, 2022). Periodontal tissue was scraped from the middle third of the root of each healthy premolar extracted by orthodontic treatment after rinsing the tooth in saline.…”

Section: Methodsmentioning

confidence: 99%

LncRNA MALAT1 promotes osteogenic differentiation through the miR‐93‐5p/SMAD5 axis

Bai

2023

Oral Diseases

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ObjectivesPromoting the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) is a way to regenerate periodontal bone. This study aimed to determine whether lncRNA MALAT1 promotes the osteogenic differentiation of human PDLSCs in vitro.Materials and MethodsHuman PDLSCs were extracted from the human periodontal ligament, and after osteogenic differentiation was induced using osteogenic medium, the human PDLSCs were transfected with siRNA‐MALAT1, miR‐93‐5p mimics, and miR‐93‐5p inhibitors. The expression of osteogenesis‐related genes was assessed by RT–qPCR and western blotting, alkaline phosphatase (ALP) activity was assessed by ALP activity assay, and the formation of mineralized nodules was assessed by alizarin red S (ARS) staining. RNA immunoprecipitation (RIP) and luciferase assays were performed to assess the binding of MALAT1, miR‐93‐5p, and SMAD5.ResultsThe expression of lncRNA metastasis‐associated lung adenocarcinoma transcript 1 (MALAT1) was upregulated, while that of miR‐93‐5p was downregulated after PDLSC osteogenic differentiation. Knockdown of MALAT1 inhibited the osteogenic differentiation of PDLSCs, and MALAT1 expression negatively correlated with miR‐93‐5p expression. miR‐93‐5p inhibited the osteogenic differentiation of human PDLSCs by specifically binding to SMAD5.ConclusionMALAT1 regulates human PDLSC differentiation by regulating the miR‐93‐5p/SMAD5 axis.

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Section: Methodsmentioning

confidence: 99%

LncRNA MALAT1 promotes osteogenic differentiation through the miR‐93‐5p/SMAD5 axis

Bai

2023

Oral Diseases

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“…DFSCs can differentiate into cementoblasts in vivo [ 85 ] and they can also be directed to differentiate into conventional multidirectional lineages [ 8 , 86 ]. Instead, GMSCs can be targeted for osteogenic, adipogenic, chondrogenic, neurogenic, endothelial-like, odontogenic, and myogenic differentiation [ 46 , 47 ].…”

Section: Maintenance and Survival Of Dscsmentioning

confidence: 99%

Analogies and Differences Between Dental Stem Cells: Focus on Secretome in Combination with Scaffolds in Neurological Disorders

Santilli,

Fabrizi,

Santacroce

et al. 2023

Stem Cell Rev and Rep

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Mesenchymal stem cells (MSCs) are well known for their beneficial effects, differentiation capacity and regenerative potential. Dental-derived MSCs (DSCs) are more easily accessible and have a non-invasive isolation method rather than MSCs isolated from other sources (umbilical cord, bone marrow, and adipose tissue). In addition, DSCs appear to have a relevant neuro-regenerative potential due to their neural crest origin. However, it is now known that the beneficial effects of MSCs depend, at least in part, on their secretome, referring to all the bioactive molecules (neurotrophic factors) released in the conditioned medium (CM) or in the extracellular vesicles (EVs) in particular exosomes (Exos). In this review, we described the similarities and differences between various DSCs. Our focus was on the secretome of DSCs and their applications in cell therapy for neurological disorders. For neuro-regenerative purposes, the secretome of different DSCs has been tested. Among these, the secretome of dental pulp stem cells and stem cells from human exfoliated deciduous teeth have been the most widely studied. Both CM and Exos obtained from DSCs have been shown to promote neurite outgrowth and neuroprotective effects as well as their combination with scaffold materials (to improve their functional integration in the tissue). For these reasons, the secretome obtained from DSCs in combination with scaffold materials may represent a promising tissue engineering approach for neuroprotective and neuro-regenerative treatments. Graphical Abstract

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Comparative analysis of proliferative and multilineage differentiation potential of human periodontal ligament stem cells from maxillary and mandibular molars

Mert

Malyaran

Craveiro

et al. 2023

Journal of Periodontology

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Background: Clinical experience indicates that wounds in alveolar bone and periodontal tissue heal faster and more efficiently in the maxilla compared with the mandible. Since stem cells are known to have a decisive influence on wound healing and tissue regeneration, the aim of this study was to determine whether differences in proliferation and differentiation of periodontal ligament stem cells (PDLSC) from upper (u-PDLSC) and lower jaw (l-PDLSC) contribute to the enhanced wound healing in the maxilla. Methods: u-PDLSC and l-PDLSC from the same donor were harvested from the periodontal ligament of extracted human maxillary and mandibular third molars. Cell differentiation potential was assessed by analyzing stem cell markers, proliferation rate, and multilineage differentiation among each other and bone marrow-derived mesenchymal stem cells (MSC). Successful differentiation of PDLSC and MSC toward osteoblasts, adipocytes, and chondrocytes was analyzed via reverse transcriptase-quantitative polymerase chain reaction and histochemical staining (Alizarin Red, Oil Red O, Toluidine Blue).Results: u-PDLSC and l-PDLSC expressed the MSC-markers CD73 + , CD90 + , and CD105 + and lacked expression of CD34 − and CD45 − . Proliferation was significantly higher in u-PDLSC than in l-PDLSC, regardless of the culture conditions. Osteogenic (ALP, RunX2, and osteocalcin) and chondrogenic (SOX9 and ACAN) related gene expression as well as staining intensities were significantly Sinan Mert and Hanna Malyaran contributed equally to this work.

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Comparison of Cellular and Differentiation Characteristics of Mesenchymal Stem Cells Derived from Human Gingiva and Periodontal Ligament

Cited by 9 publications

References 27 publications

LncRNA MALAT1 promotes osteogenic differentiation through the miR‐93‐5p/SMAD5 axis

LncRNA MALAT1 promotes osteogenic differentiation through the miR‐93‐5p/SMAD5 axis

Analogies and Differences Between Dental Stem Cells: Focus on Secretome in Combination with Scaffolds in Neurological Disorders

Comparative analysis of proliferative and multilineage differentiation potential of human periodontal ligament stem cells from maxillary and mandibular molars

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