Comparison of Cell Response and Surface Characteristics on Titanium Implant with SLA and SLAffinity Functionalization

Abstract

To evaluate the cell responses of titanium oxide layer with the nanoporous micro-structure, an electrochemical anodization approach was proposed to create a nanoporous structure that superimposed onto the moderately rough sand-blasted, large-grit, acid-etched (SLA) Ti surface (denoted as SLAffinity-Ti). The osteoblast-like cells were cultured on SLAffinity-Ti and un-treated surfaces. Cell adhesion, viability, mRNA expressions as well as the productions of alkaline phosphatase (ALP) and osteocalcin (OC) were evaluated at predetermined intervals. The present results showed that cells had a high viability, attachment, and improved cell proliferation at day 7 and 14 when cultured on SLAffinity-Ti surface. The mRNA expressions of ALP and OC were upregulated while the type I collagen was not influenced. Additionally, the cells cultured on the SLAffinity-Ti surface produced a higher level of ALP and OC relative to cells on untreated surfaces. These findings confirmed that surface topography (nanoporous roughness) and TiO 2 layers influenced the cell responses and the study revealed that cells grown on SLAffinity-Ti may improve osseointegration for Ti implants.

Early bone response to machined, sandblasting acid etching (SLA) and novel surface‐functionalization (SLAffinity) titanium implants: characterization, biomechanical analysis and histological evaluation in pigs

“…Surface modifications of implants, which are affected by roughness or by altering the chemical composition, boost appropriate biological interactions between the tissue and the implants, ultimately accelerating osseointegration. Recently, much attention has been focused on hybrid topography, consisting of micropits and nanoporous TiO 2 layers achieved via electrochemical oxidation, to mimic the natural bony environment . Electrochemical oxidation results in increased wettability, and affects the chemical composition of standard sandblasted, large grit, acid‐etched (SLA) surfaces.…”

Follow-up Study of Dental Implants With Bioactive Oxide Films on Bone Tissue Healing and Osseointegration

“…Oxygen depth profiles for 3 types of Ti surfaces using x-ray photoelectron spectra. 13 It was found that the spontaneously air-formed oxide layer on P-Ti surface was approximately 12 nm in thickness, whereas the thickness of oxide layer resulted from SLA-Ti treatment was about 16 nm. Fig.…”

“…2). 13 It was found that the spontaneously air-formed oxide layer on P-Ti surface was approximately 12 nm in thickness, whereas the thickness of oxide layer resulted from SLA-Ti treatment was about 16 nm. As compared with other surfaces, the SLAffinity-Ti surface showed a significantly thicker oxide layer, whose diffused thickness was around 500 nm (P ¼ 0.0063).…”

Polymeric nanoparticles for therapy and imaging

“…Our recent results have indicated that biodegradable NPs have great capacity as tracking agent to transfect stem cells for regenerative medicine therapy . Alternatively, other research indicated that these NPs have many other applications in targeted delivery system such as cancer treatment …”