Comparison of cell invasion, macrophage survival and inflammatory cytokines profiles between Salmonella enterica serovars Enteritidis and Dublin from Brazil

Abstract

Aims This study compared the capacity of strains of Salmonella enterica serovars Enteritidis and Dublin isolated in Brazil to invade epithelial cells, to be internalized by and survive within macrophages, and to stimulate cytokine release in vitro. Methods and Results Both serovars infected 75 and 73% Caco‐2 (human) and MDBK (bovine) epithelial cells respectively. Salmonella Dublin and S. Enteritidis (i) were internalized at the respective rates of 79·6 and 65·0% (P ≤ 0·05) by U937 (human) macrophages, and 70·4 and 66·9% by HD11 (chicken) macrophages; and (ii) multiplied at the respective rates of 3·2‐ and 2·7‐fold within U937 cells, and 1·9‐ and 1·1‐fold (P ≤ 0·05) within HD11 cells respectively. Seventy per cent of 10 S. Dublin strains stimulated IL‐8 production, while 70% of S. Enteritidis strains enhanced production of IL‐1β, IL‐6, IL‐8, IL‐10, IL‐12p70 and TNF in Caco‐2 cells. Conclusions Compared with S. Enteritidis, S. Dublin had stronger ability to survive within macrophages and induced weak cytokine production, which may explain the higher incidence of invasive diseases caused by S. Dublin in humans. Significance and Impact of the STUDY This study compared S. enterica serovars Enteritidis and Dublin to provide comparative data about the profile of the two serovars in cells from humans, the common host and their respective natural animal hosts and vice versa in order to check the differences between these two phylogenetically closely related serovars that share antigenic properties but present different phenotypic behaviours.

Unraveling the role of type 1 fimbriae in Salmonella pathogenesis: insights from a comparative analysis of Salmonella Enteritidis and Salmonella Gallinarum

“…Infantis, S . Dublin and S. Gallinarum infecting HD11 cells or chicken peripheral blood mononuclear cell-derived ( Stabler et al, 1994 ; Okamura et al, 2005 ; He et al, 2012 ; Blondel et al, 2013 ; Braukmann et al, 2015 ; Campioni et al, 2021 ). Our results obtained with macrophage-like HD11 cells revealed that the total intracellular S .…”

Persistent ciprofloxacin exposure induced the transformation of Klebsiella pneumoniae small colony variant into mucous phenotype

“…After that, the cells were treated with 0.5% Triton X-100 in PBS for 10 minutes, and the supernatant was diluted and plated onto TSB agar plates for overnight culture at 37°C. The differences in the colony counts of KPN in each group were observed ( Campioni et al., 2021 ).…”

Genomic epidemiology and phenotypic characterisation ofSalmonella entericaserovar Panama in Victoria, Australia

“…Schwarzengrund, S. Heidelberg, S. Virchow, S. Infantis, and S. Panama (9). Previous studies have largely focussed on S. Typhimurium, S. Enteritidis and S. Dublin (10)(11)(12)(13)(14)(15)(16).…”