Comparison of Cell-Free Fetal DNA Plasma Content Used to Sex Determination Between Three Trimesters of Pregnancy in Torkaman Pregnant Mare

Tonekaboni, Fahimeh Rezaei; Reza, Narenjisani; Staji, Hamid; Mahmood, Asif

doi:10.1016/j.jevs.2020.103273

Cited by 5 publications

(3 citation statements)

References 32 publications

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“…(2020) fetal sex with good accuracy through cffDNA analysis has revealed 100% specificity in all duration of pregnancy. [ 12 ] Due to the dependence of the accuracy of ultrasound on the type of machine and the skill of the operator, it is recommended that studies performed to examine the criteria and determine their accuracy in relation to sex determination.…”

Section: Discussionmentioning

confidence: 99%

The impact of maternal predisposing factors on level of maternal serum pregnancy-associated plasma protein A and free subunit human chorionic gonadotropin and nuchal translucency

2023

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Background: This study aimed to investigate the relationship between maternal predisposing factors with the level of maternal serum pregnancy-associated plasma protein A and free subunit human chorionic gonadotropin and nuchal translucency. Materials and Methods: We performed a cross-sectional-analytical study on 762 pregnant women who referred to the Gene Azma Medical Genetics Laboratory in Isfahan for amniocentesis. All pregnant women at high risk of screening in the first trimester of pregnancy for trisomy 21 and other aneuploidy were referred to a gynecologist for amniotic fluid sampling (amniocentesis). Multiple of the means (MoM) of PAPPA ≤0.5, 0.5 ≥ MoM free β-hCG >2.5, and NT ≥3.5 mm were considered abnormal. We used Chi-square method and Mann–Whitney U-test to compare data qualitative and quantitative, respectively. Results: In individuals with less pregnancies and deliveries, the value of abnormal NT was higher ( P < 0.01, P < 0.001, respectively). On the other hand, the highest abnormal rate of NT was observed in pregnant women under 35 years (21, 84%, P < 0.012). In addition, abnormal levels of free β -hCG are more common in women < 35 years of age (186, 66.9%, P < 0.02) and female fetuses (171, 58.8%) ( P < 0.006). Conclusion: According to the results of this study, it can be said that considering the underlying factors of pregnant mothers in performing tests related to screening in the first trimester of pregnancy can lead to a reduction in false positive rates.

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Section: Discussionmentioning

confidence: 99%

The impact of maternal predisposing factors on level of maternal serum pregnancy-associated plasma protein A and free subunit human chorionic gonadotropin and nuchal translucency

2023

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“…In conclusion, research in this field in the horse is very much in its infancy but shows great promise for future diagnostic tests and to inform breeding decisions that avoid lethality. Research on techniques such as non-invasive prenatal testing through the detection of foetally derived DNA in pregnant mare serum has been initiated (Tonekaboni et al 2020;Kadivar et al 2021) and based on its success in women (Guy et al 2021), is likely to be valuable to make this research relevant to clinical practice. To date, there are no foetal only or placental only variants, although it is reasonable to assume with further research these will be revealed in due course.…”

Section: Final Discussion and Concluding Commentsmentioning

confidence: 99%

Lethal variants of equine pregnancy: is it the placenta or foetus leading the conceptus in the wrong direction?

Shilton

Kahler²,

Roach³

et al. 2022

Reprod. Fertil. Dev.

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Embryonic and foetal loss remain one of the greatest challenges in equine reproductive health with 5-10% of established day 15 pregnancies and a further 5-10% of day 70 pregnancies failing to produce a viable foal. The underlying reason for these losses is variable but ultimately most cases will be attributed to pathologies of the environment of the developing embryo and later foetus, or a defect intrinsic to the embryo itself that leads to lethality at any stage of gestation right up to birth. Historically, much research has focused on the maternal endometrium, endocrine and immune responses in pregnancy and pregnancy loss, as well as infectious agents such as pathogens, and until recently very little was known about the both small and large genetic variants associated with reduced foetal viability in the horse. In this review, we first introduce key aspects of equine placental and foetal development. We then discuss incidence, risk factors and causes of pregnancy loss, with the latter focusing on genetic variants described to date that can impact equine foetal viability.

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“…When comparing to postnatal monkeys, the results validated the SRY and DYS14 sequences on the Y chromosome in 28 monkeys, obtaining 100% sex correctness. Since its inception, cffDNA has been used to determine prenatal fetal sex in a variety of mammals, including cattle (Lemos et al, 2011;da Cruz et al, 2012;Ristanic et al, 2018), sheep ( Kadivar et al, 2013;Asadpour et al, 2015), mare (de Leon et al, 2012;Tonekaboni et al, 2020), elephant (Vincze et al, 2019) and rhinoceros (Stoops et al, 2018). According to da Cruz et al, (2012) and Wang et al(2010), polymerase chain reaction (PCR) analysis of fetal DNA in maternal plasma of 35 pregnant calves ranging in age from 5 to 35 weeks allowed them to determine the sex of the fetus at various stages of pregnancy in cows.…”

Section: Introductionmentioning

confidence: 99%

Cattle fetal sex determination using cell-free fetal DNA from maternal blood in the Mekong Delta, Vietnam

Tran,

Nguyen,

Nguyen

et al. 2023

VIS

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Cell-free fetal DNA (cffDNA) from fetal calves was isolated in the bovine maternal plasma in the fifth week of pregnancy, accounting for around 10% of the total. Recently, cffDNA has been used as a non-invasive prenatal screening technique for aneuploidy, genetic disorders in humans, early pregnancy diagnosis, and animal fetal sex determination. This study's objective was to identify the presence of cffDNA in the blood of 13 dairy cows (11 pregnant, 2 non-pregnant, and 1 unmarred) gathered from farms in Can Tho, the Mekong Delta, Vietnam. Plasma / Serum cfc-DNA Purification Midi Kit (Product # 55600) was used to separate cffDNA. Two PCR-designed primers named SRY-137 and GAPDH-109 were employed to amplify the Y-chromosome-specific sequences SRY and the housekeeping gene GAPDH, respectively. The fetal sex was indicated by PCR data corresponding to the calf's sex at birth in 11 cases. The study's findings first proved the existence of cell-free fetal DNA circulating in the plasma of a pregnant cow in Vietnam and established the calf's sex using simple, high-precision procedures. The use of cffDNA for sex screening brings up the possibility of quickly determining the gender and screening for genetic diseases in a big herd of cattle and animals with long gestation periods.

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Comparison of Cell-Free Fetal DNA Plasma Content Used to Sex Determination Between Three Trimesters of Pregnancy in Torkaman Pregnant Mare

Cited by 5 publications

References 32 publications

The impact of maternal predisposing factors on level of maternal serum pregnancy-associated plasma protein A and free subunit human chorionic gonadotropin and nuchal translucency

The impact of maternal predisposing factors on level of maternal serum pregnancy-associated plasma protein A and free subunit human chorionic gonadotropin and nuchal translucency

Lethal variants of equine pregnancy: is it the placenta or foetus leading the conceptus in the wrong direction?

Cattle fetal sex determination using cell-free fetal DNA from maternal blood in the Mekong Delta, Vietnam

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