Comparison of cell culture and three enzyme-linked immunosorbent assays for the rapid diagnosis of respiratory syncytial virus from nasopharyngeal aspirate and tracheal secretion specimens

Ahluwalia, Gurmukh S.; Hammond, Gregory

doi:10.1016/0732-8893(88)90028-4

Cited by 13 publications

(7 citation statements)

References 11 publications

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“…This is reinforced in our study by the fact that RSV was detected by both rapid diagnostic methods, but not by CC, in 27 of 27 patients with a clinical diagnosis compatible with an RSV infection. Since it is well known that some positive ETA results may be falsely positive (10), several investigators confirmed the specificity of EIA-positive but CC-negative results by a blocking assay (1,3,11,18). Because there was insufficient quantity of such specimens left in our study, we could not document whether they were indeed true positives by using a blocking assay.…”

Section: Notesmentioning

confidence: 96%

Validation of respiratory syncytial virus enzyme immunoassay and shell vial assay results

1994

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The Pathfinder respiratory syncytial virus (RSV) enzyme immunoassay (EIA) (Kallestad), the shell vial (SV) technique, and conventional cell culture (CC) were compared for detection of RSV in nasopharyngeal aspirates. We found sensitivities, specificities, and positive and negative predictive values of 58.4, 100, 100, and 68.2%; 80.7, 97.2, 97.0, and 81.9%; and 77.6, 97.2, 96.9, and 79.5% for the CC, EIA, and SV methods, respectively. The SV and ETA techniques were both more sensitive than CC (P < 0.001). Finally, 29 respiratory viruses other than RSV were identified by CC.

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Section: Notesmentioning

confidence: 96%

Validation of respiratory syncytial virus enzyme immunoassay and shell vial assay results

1994

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“…Seasonal rises in respiratory syncytial virus (RSV) cause outbreaks of acute lower respiratory tract infections that can be severe in the young and immunocompromised patient (2,5). The increased use of ribavirin for the treatment of RSV has mandated the use of accurate, rapid laboratory procedures (3,5,11,13,15).…”

mentioning

confidence: 99%

“…The success of these procedures, however, depends on many variables. The accuracy of results is dependent upon the collection of adequate and appropriate specimens (1,2,4,5,7,8,17,19), as well as diligent specimen handling and transportation to the laboratory. Furthermore, tests subject to interpretation require technical expertise (4,5,20).…”

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confidence: 99%

Evaluation of direct immunofluorescence, enzyme immunoassay, centrifugation culture, and conventional culture for the detection of respiratory syncytial virus

Johnston

Siegel

1990

J Clin Microbiol

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Four methods of detecting respiratory syncytial virus (RSV) from clinical specimens were evaluated. A total of 410 specimens consisting of nasopharyngeal washes, aspirates, and swabs were simultaneously tested for the presence of RSV by direct immunofluorescence assay (DFA), enzyme immunoassay (EIA) (Kallestad Pathfinder), shell vial centrifugation culture (SVC), and conventional culture. DFA identified 146 (83%) of the 175 positive cases, EIA detected 153 (87%), SVC detected 127 (73%), and conventional culture detected 70 (40%). Conventional culture isolated an additional 19 respiratory viruses other than RSV. DFA and EIA were able to detect nonviable virus not isolated by a culture method, and SVC isolated low-titer virus not detected by conventional culture. DFA and EIA gave similar results; however, the EIA system was less dependent on technical expertise. The use of SVC enhanced the conventional culture system with 63 RSV isolates not recovered from the tube culture. We recommend complementary use of both culture and nonculture methods in the detection of RSV. * Corresponding author. distance some specimens had to travel before they arrived at the laboratory. Viral isolation in conventional tissue culture tubes and in centrifugation cultures was complemented with the use of DFA and an EIA system (Kallestad Pathfinder; Kallestad Diagnostics, Chaska, Minn.).

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“…In the PRI.DE study, we relied on the commonly agreed assumption that NPS provides the most efficient sampling method [50,51].…”

Section: Discussionmentioning

confidence: 99%

The use of imperfect diagnostic tests had an impact on prevalence estimation

Ihorst

Förster

Petersen³

et al. 2007

Journal of Clinical Epidemiology

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Comparison of cell culture and three enzyme-linked immunosorbent assays for the rapid diagnosis of respiratory syncytial virus from nasopharyngeal aspirate and tracheal secretion specimens

Cited by 13 publications

References 11 publications

Validation of respiratory syncytial virus enzyme immunoassay and shell vial assay results

Validation of respiratory syncytial virus enzyme immunoassay and shell vial assay results

Evaluation of direct immunofluorescence, enzyme immunoassay, centrifugation culture, and conventional culture for the detection of respiratory syncytial virus

The use of imperfect diagnostic tests had an impact on prevalence estimation

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