Comparison of cell behavior on pva/pva-gelatin electrospun nanofibers with random and aligned configuration

Huang, Chen Yu; Hu, Keng-Hsiang; Wei, Zung‐Hang

doi:10.1038/srep37960

Cited by 128 publications

(77 citation statements)

References 20 publications

(21 reference statements)

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“…Cell organisation on cryogenic scaffold acted similarly to aligned scaffolds in that cells attached themselves along individual fibres. These results are analogous to those presented by Huang et al (2016) who showed an increased interaction of cells with PVA-gelatin scaffolds compared to PVA alone where spreading of cells suggests good adhesion between ECM proteins and scaffold. This cell spreading on plasma treated electrospun PCL scaffolds is beneficial to groups studying the effect of external mechanical stimulus on cells, especially in the case of aligned scaffolds.…”

Section: Discussionsupporting

confidence: 78%

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The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

2017

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There is a pressing need for further advancement in tissue engineering of functional organs with a view to providing a more clinically relevant model for drug development and reduce the dependence on organ donation. Polymer based scaffolds, such as polycaprolactone (PCL), have been highlighted as a potential avenue for tissue engineered kidneys, but there is little investigation down this stream.Focus within kidney tissue engineering has been on 2-dimensional cell culture and decellularised tissue. Electrospun polymer scaffolds can be created with a variety of fibre diameters and have shown a great potential in many areas. The variation in morphology of tissue engineering scaffold has been shown to effect the way cells behave and integrate. In this study we examined the cellular response to scaffold architecture of novel electrospun scaffold for kidney tissue engineering. Fibre diameters of 1.10±0.16 µm and 4.49±0.47 µm were used with 3 distinct scaffold architectures. Traditional random fibres were spun onto a mandrel rotating at 250 rpm, aligned at 1800 rpm with novel cryogenic fibres spun onto a mandrel loaded with dry ice rotating at 250 rpm. Human kidney epithelial cells were grown for 1 and 2 weeks. Fibre morphology had no effect of cell viability in scaffolds with a large fibre diameter but significant differences were seen in smaller fibres. Fibre diameter had a significant effect in aligned and cryogenic scaffold. Imaging detailed the differences in cell attachment due to scaffold differences. These results show that architecture of the scaffold has a profound effect on kidney cells; whether that is effects of fibre diameter on the cell attachment and viability or the effect of fibre arrangement on the distribution of cells and their alignment with fibres. Results demonstrate that PCL scaffolds have the capability to maintain kidney cells life and should be investigated further as a potential scaffold in kidney tissue engineering.

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Section: Discussionsupporting

confidence: 78%

“…We have seen distinct morphological differences in cells grown on different architectures, similar to other findings (Yan et al 2012;Mahjour et al 2016;Huang et al 2016). Our random scaffolds show cells in a more rounded morphology, spread between numerous fibres.…”

Section: Discussionsupporting

confidence: 76%

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

2017

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“…However, the add it ion of PVA seems to create some regular globs with various sizes caused by different rat ios of PVA [14]. Th is results may be attributed to the non-adhesive property of PVA, wh ich restricts the adhesion and spread o f the CM C [15].…”

Section: Resultsmentioning

confidence: 99%

Molecularly Conductive Behavior of Blended Polymer Electrolyte-based CMC/PVA

Ali¹,

Zhang²,

Nagao³

et al. 2019

MST

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This study investigated the electrical conduction and structural behavior of blended polymer electrolyte (BPE)-based carboxymethyl cellulose (CMC) and polyvinyl alcohol (PVA) in the development of solid-state electrochemical devices. Based on impedance spectroscopy and correlating Fourier transform infrared (FTIR) with thermogravimetric analysis, a framework was proposed to explain the structural enhancement of the BPE system. As revealed by FTIR, the optimum conductivity of CMC/PVA BPEs was 9.21 × 10−6 Scm−1 for 80:20 composition attributed to the intermolecular attraction between the polymers. Thermal stability of the CMC/PVA was influenced by the formation of a hydrogen bond between the hydroxyl (-OH), carboxylate (-COO-), and ether linkage (-COC-) functional groups. The finding provides insights into blended polymer electrolyte-based CMC/PVA, which is beneficial in designing safe, thin, and lightweight energy storage devices. AbstractPenilaian Kekonduksian dan CMC/PVA Filem Nipis Polimer Campuran. Karya ini menyelidiki penemuan pengaliran elektrik dan tingkah laku struktur elektrolit polimer campuran (BPEs) berasaskan carboxymethyl cellulose (CMC) dan polivinil alkohol (PVA) ke arah pembangunan peranti elektrokimia keadaan pepejal. Berdasarkan spektroskopi impedans (EIS) dan mengaitkan fourier transform inframerah (FTIR) dengan analisis thermogravimetric (TGA), kami telah mencadangkan satu rangka kerja untuk menjelaskan peningkatan struktur sistem BPE. Kekonduksian optima CMC/PVA BPEs dicapai ialah 9.21 x 10 -6 Scm -1 untuk komposisi 80:20 yang dikaitkan dengan tarikan dengan molekul antara polimer yang telah didedahkan oleh FTIR. Kestabilan haba CMC/PVA didapati dipengaruhi oleh pembentukan ikatan hidrogen antara kumpulan berfungsi hidroksil (-OH), karboksilat (-COO -) dan rantaian ether (-COC-). Penemuan ini memberi beberapa pandangan tentang polimer campuran yang diadaptasi berasaskan CMC/PVA yang bermanfaat dalam mereka bentuk peranti simpanan yang selamat, nipis dan ringan.

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“…The absorption peaks appeared at ∼1640 and ∼1400 cm −1 is most probably, either due to C=C and C-C stretching, respectively, or from the symmetric and asymmetric stretching vibration of the C=O/C-O group. The absorption peaks appeared at ∼3000 cm −1 is due to the symmetric and asymmetric stretching vibration of CH 2 [29,30]. The absorption bands appeared at ∼1300 and ∼940 cm −1 was ascribed to symmetric C-H and bending vibration of -CH 3 group, respectively.…”

Section: Ft-ir Analysismentioning

confidence: 95%

Porous PVA/Zn–Fe–Mn oxide nanocomposites: methylene blue dye adsorption studies

Abebe

Murthy

Zereffa

et al. 2020

Mater. Res. Express

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Adsorption is one of the noble techniques for remediation of organic and inorganic pollutants. The poly (vinyl alcohol) supported sol-gel and self-propagation routes have been used for the synthesis of porous ternary metal oxides nanocomposites. The optical, chemical bonding, crystallinity, morphological, textural, and electrochemical properties of the synthesized materials were characterized by DRS-UV-vis, FT-IR, XRD, SEM/EDX and TEM/HRTEM/SAED, BET, and CV/EIS techniques, respectively. The characterization of the nanocomposites confirmed their porous nature, high surface area, and better electrochemical properties. The synthesized nanomaterials were tested for the adsorption property of methylene blue dye. Important parameters such as the amount of PVA supported ternary metal oxide nanocomposite, pH of the solution, contact time, and concentration of methylene blue dye were optimized. For further understanding of the adsorption process, the adsorption isotherms and adsorption kinetics models were used. The adsorption tests revealed the presence of the chemisorption type of the adsorption process. several advantageous properties [8,9]. Besides, its hydroxyl groups on the carbon chain backbone act as a source for the formation of hydrogen bonding and enhances the formation of the nanocomposite [10]. As confirmed by various researchers [11][12][13][14][15][16], around 250, 300, 390, 400, and 400°C-500°C; the intramolecular decomposition, amorphous portion degradation, intermolecular breakdown, decaying of the crystalline portion of PVA, and formation of pure metal oxides had occurred, respectively.From methods used for the synthesis of inorganic nanomaterials/nanocomposites, the top-down and bottom-up approaches are common. Compared to the former, the latter is more popular and is considered as a promising route to control the growth, morphology, and properties of the nanomaterials. From the bottom-up chemical approaches; the hydrothermal, microemulsion, wet impregnation, sonochemical, ultrasonicmicrowave, electrochemical/electroless deposition, and spray-pyrolysis techniques are successfully applied. However, it is reported that these techniques follow special operating-conditions, long reaction time, lowthrough-put, and expensive procedure [17]. Therefore, it is essential to use effective and economically feasible methods such as sol-gel, precipitation, and impregnation. Furthermore, during the synthesis process, avoiding solvents that cause cancer and has mutagenic properties should also be taken into consideration [18].Considering all the mentioned aspects, this work was aimed to synthesize PVA supported ternary metal oxide nanocomposite (PVA-TMONC), using a simple and green sol-gel-self propagation technique. The methylene blue dye (MBD) adsorption efficiency of the synthesized PVA-TMONC was also tested. The physical properties of synthesized materials were characterized by critical analytical techniques. Using the Langmuir, Freundlich, Dubinin-Radushkevich (D-RK), Temkin, Flory-Huggins (FH), and Fowler-Gugge...

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Comparison of cell behavior on pva/pva-gelatin electrospun nanofibers with random and aligned configuration

Cited by 128 publications

References 20 publications

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

The effect of electrospun polycaprolactone scaffold morphology on human kidney epithelial cells

Molecularly Conductive Behavior of Blended Polymer Electrolyte-based CMC/PVA

Porous PVA/Zn–Fe–Mn oxide nanocomposites: methylene blue dye adsorption studies

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