Comparison of cELISA and IFA tests in the serodiagnosis of anaplasmosis in cattle

Ekici, Ozlem Derinbay; Sevinç, Ferda

doi:10.5897/ajmr11.007

Cited by 10 publications

(2 citation statements)

References 18 publications

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“…in sheep [13,40,41]. Recently, Ab-IFAT was shown to have high sensitivity (100%) and specificity (91.9%) when compared to the combination of microscopic examination and cELISA, while a previous study reported a similar level of specificity and sensitivity for Ab-IFAT when compared with cELISA [15,17].…”

Section: Discussionmentioning

confidence: 97%

“…On the contrary, diagnosis through blood smear cytological examination may not be feasible to identify carrier animals. Thus, serological assays, such as indirect immunofluorescence antibody (Ab-IFAT), enzyme-linked immunosorbent assay (ELISA) and complement fixation tests (CF), have been used for the detection of anti-Anaplasma antibodies [11][12][13][14][15]. Among the various serological techniques, competitive ELISA (cELISA) presents high sensitivity and specificity for the detection of Anaplasma-specific antibodies [16], while the Ab-IFAT has been commonly used in epidemiological studies.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of an Indirect Immunofluorescence Assay for the Detection of Anaplasma phagocytophilum Antigen in Ovine Buffy Coat Smears

et al. 2022

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Diagnosis of anaplasmosis is challenging considering the great variation in clinical signs and the limitations of the available diagnostic assays, while the detection of carrier animals that play a significant role in disease epidemiology as reservoirs is of great significance. In this study, we evaluated the diagnostic accuracy of a newly developed indirect immunofluorescent assay (Ag-IFAT) for the detection of A. phagocytophilum antigens in buffy coat specimens, alone and in combination with cytology, using PCR as a reference. Blood samples were collected from 138 sheep of the Chios breed from six farms in Greece. A buffy coat was extruded from the centrifuged blood. Buffy coat smears were used for cytological examination and the Ag-IFAT assay. The Ag-IFAT assay presented excellent specificity (100%) and high sensitivity (85.4%) for the detection of A. phagocytophilum antigens in buffy coats, and it has an almost perfect agreement with PCR and cytology (κ value = 0.88 and 0.85, respectively). A. phagocytophilum antigens are likely to be detected using Ag-IFAT in a PCR-positive animal, as indicated by the good performance of the assay. Overall, this assay presents high diagnostic accuracy, and it could be used for the detection of animals during the early stage of infection.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Evaluation of an Indirect Immunofluorescence Assay for the Detection of Anaplasma phagocytophilum Antigen in Ovine Buffy Coat Smears

et al. 2022

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Molecular detection and genetic identification of Babesia bigemina, Theileria annulata, Theileria orientalis and Anaplasma marginale in Turkey

Zhou

Cao

Sevinç

et al. 2016

Ticks and Tick-borne Diseases

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Diagnostic tools of caprine and ovine anaplasmosis: a direct comparative study

2018

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BackgroundThe diagnosis of anaplasmosis is rather conflicting with other haemoprotozoans. Hence, the study aimed to compare and evaluate the efficiency of competitive ELISA (cELISA), indirect fluorescence antibody (IFA), and Polymerase chain reaction (PCR) for precise diagnosis of Anaplasma spp. and to assess their concordance with microscopic examination (ME).ResultsA total of 312 blood samples (189 sheep and 123 goats) were examined for Anaplasma infection during a 1 year period. Giemsa-stained blood smears were examined under the microscope. IFA and cELISA were used for the detection of Anaplasma spp. antibodies. PCR was used as a standard of truth and for the identification of Anaplasma species. Using cELISA assay, 47.4% (148) were positive (93 sheep and 55 goats) with a sensitivity and specificity of 91.9, and 86.9%, respectively. Using IFA, it was found that 57.4% (179)were positive (113 sheep and 66 goats) with a sensitivity and specificity of 100, and 93.3%, respectively. PCR assay identified A. ovis in 49 (25.3%) sheep and 30 (15.5%) goats, and A. phagocytophilumin 74 (38.1%) sheep and 41 (20.8%) goats.ConclusionsHigh sensitivity and specificity values of IFA and ELISA tests compared to microscopic examination strongly support their utility in the diagnosis of Anaplasma infection. PCR was a more specific diagnostic tool that allows to discriminate between Anaplasma subspecies, which makes it the method of choice for anaplasmosis diagnosis.

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Comparison of cELISA and IFA tests in the serodiagnosis of anaplasmosis in cattle

Cited by 10 publications

References 18 publications

Evaluation of an Indirect Immunofluorescence Assay for the Detection of Anaplasma phagocytophilum Antigen in Ovine Buffy Coat Smears

Evaluation of an Indirect Immunofluorescence Assay for the Detection of Anaplasma phagocytophilum Antigen in Ovine Buffy Coat Smears

Molecular detection and genetic identification of Babesia bigemina, Theileria annulata, Theileria orientalis and Anaplasma marginale in Turkey

Diagnostic tools of caprine and ovine anaplasmosis: a direct comparative study

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