Comparison of cefoxitin and moxalactam 30 µg disc diffusion methods for detection of methicillin resistance in coagulase-negative staphylococci

Join‐Lambert, Olivier; Clauser, Sylvain; Guillet, Christelle; Jaı̈s, Jean-Philippe; Abachin, Éric; Quesnes, Gilles; Carbonnelle, Étienne; Monnier, Alban Le; Zahar, Jean‐Ralph; Kayal, Samer; Berche, Patrick; Ferroni, Agnès

doi:10.1093/jac/dkl548

Journal of Antimicrobial Chemotherapy

2007

DOI: 10.1093/jac/dkl548

|View full text |Cite

Comparison of cefoxitin and moxalactam 30 µg disc diffusion methods for detection of methicillin resistance in coagulase-negative staphylococci

Olivier Join‐Lambert¹,

Sylvain Clauser²,

Christelle Guillet³

et al.

Abstract: Although all DD methods studied here performed well for detecting methicillin resistance in CoNS, moxalactam had a higher accuracy than cefoxitin to differentiate heteroresistant isolates from PBP2a-negative strains. Identification of isolates that should be submitted to a confirmatory test to conclude on methicillin resistance can be easily obtained by combining cefoxitin and moxalactam DD methods.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2007

2016

Publication Types

Select...

Article9

Relationship

Self Cite0

Independent9

Authors

Journals

Cited by 13 publications

(19 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One group recommended the use of a combination of oxacillin MIC and moxalactam MIC tests (7); the other recommended the use of a combination of cefoxitin disk and moxalactam disk tests (5). The use of moxalactam tests in combination with cefoxitin and/or oxacillin tests to predict the mecA status of CoNS may deserve further study, given that the use of cefoxitin MICs alone is not optimal for this purpose.…”

mentioning

confidence: 99%

Correlation of Cefoxitin MICs with the Presence of mecA in Staphylococcus spp

Swenson

Brasso²,

Ferraro

et al. 2009

J Clin Microbiol

View full text Add to dashboard Cite

This report describes the results of an 11-laboratory study to determine if a cefoxitin broth microdilution MIC test could predict the presence of mecA in staphylococci. Using breakpoints of <4 g/ml for mecA-negative and >6 or 8 g/ml for mecA-positive isolates, sensitivity and specificity based on mecA or presumed mecA for Staphylococcus aureus at 18 h of incubation were 99.7 to 100% in three cation-adjusted Mueller-Hinton broths tested. For coagulase-negative strains at 24 h of incubation, breakpoints of <2 g/ml for mecA-negative and >4 g/ml for mecA-positive isolates gave sensitivity and specificity of 94 to 99% and 69 to 80%, respectively.The use of a cefoxitin disk test to detect staphylococci that are likely to contain the mecA gene has been widely advocated since the test was first suggested (3, 6) and has been adopted by antimicrobial susceptibility testing organizations worldwide (http://www.bsac.org.uk/_db/_documents/version_6.1.pdf, http: //www.srga.org/, http://www.clsi.org/). For laboratories that do not use disk diffusion as their primary testing method, performing disk diffusion requires additional expense and reagents. Consequently, we performed studies to determine if a cefoxitin broth microdilution MIC breakpoint would correlate with the presence of the mecA gene in staphylococci.Cation-adjusted Mueller-Hinton broths (CAMHB) from three manufacturers (BBL, BD Diagnostic Systems, Sparks, MD; Difco, BD Diagnostic Systems, Sparks, MD; and Oxoid, Basingstoke, Hampshire, England) (cation content adjusted if necessary), were used to prepare frozen microdilution panels containing cefoxitin concentrations of 0.5 to 32 g/ml (including 6 g/ml) and oxacillin concentrations of 0.06 to 16 g/ml (in BBL CAMHB only). The frozen panels were shipped to all participants, along with 30-g cefoxitin disks (BBL). Each laboratory used its current lot of Mueller-Hinton agar for disk diffusion testing. ). Additional strains were tested at the CDC, totaling 167 S. aureus isolates and 22 CoNS. The total number of strains tested in all labs was 479 S. aureus isolates and 204 CoNS. All results were read after both 18-h and 24-h incubations. Endpoints were read as the lowest concentration at which no visible growth was observed. At 18 h of incubation, 97.3% of the MIC quality control results (for S. aureus ATCC 29213) and 97.9% of the disk diffusion quality control results (for S. aureus ATCC 25923) were within the range considered acceptable by the Clinical and Laboratory Standards Institute (CLSI).After testing, all strains were shipped to the CDC for further analysis. PCR testing for mecA was performed as previously described (8) on the following strains: (i) any S. aureus strain for which the cefoxitin MIC was Ͻ16 g/ml in any of the three media tested (n ϭ 180); (ii) any S. aureus strain for which the cefoxitin MIC was Ͼ8 g/ml and the oxacillin MIC was Ͻ4 g/ml in any of the three media tested (n ϭ 5); and (iii) a sample of the 304 isolates (n ϭ 16 [5%]) for which the cefoxitin MIC was Ͼ8 g/ml and the oxacillin MIC was Ͼ2 g...

show abstract

mentioning

confidence: 99%

Correlation of Cefoxitin MICs with the Presence of mecA in Staphylococcus spp

Swenson

Brasso²,

Ferraro

et al. 2009

J Clin Microbiol

View full text Add to dashboard Cite

show abstract

“…The relevance of the moxalactam disk is slightly better for mecA-positive strains, for which Felten et al obtained a better sensitivity compared to that achieved with cefoxitin (97% and 94%, respectively) (3). Moreover, JoinLambert et al demonstrated that the moxalactam disk better differentiated heteroresistant CoNS isolates from mecA-negative isolates (7).…”

mentioning

confidence: 99%

Evaluation of Moxalactam with the BD Phoenix System for Detection of Methicillin Resistance in Coagulase-Negative Staphylococci

et al. 2007

View full text Add to dashboard Cite

The performance of moxalactam with the BD Phoenix system for the detection of methicillin resistance in coagulase-negative staphylococci was evaluated by use of a collection of 186 strains. Moxalactam was a better drug as an indicator of methicillin resistance for mecA-positive strains than oxacillin and cefoxitin were. For strains other than Staphylococcus saprophyticus, a moxalactam MIC >16 g/ml was indicative of methicillin resistance.

show abstract

“…Therefore, the cefoxitin disc diffusion method, currently defined as a standard procedure by the European Committee on Antimicrobial Susceptibility Testing (EUCAST) and the Clinical and Laboratory Standards Institute (CLSI), may fail to detect meticillin resistance in these isolates (CLSI, 2016;. The Committee for Antibiotic Susceptibility Testing of the French Society of Microbiology (CA-SFM) introduced a 30 µg moxalactam disc in 2005, which has been proposed to be a better predictor than cefoxitin for the detection of meticillin resistance in CoNS (CA-SFM, 2012;Join-Lambert et al, 2007). We evaluated the effectiveness of various single and combined methods for the detection of meticillin resistance in CoNS, and derived a practical diagnostic approach.…”

mentioning

confidence: 99%

Improved sensitivity for meticillin resistance detection in coagulase-negative staphylococci by moxalactam antibiotic discs or a cefoxitin investigation zone

Jost

Bloemberg

Hombach

2016

Journal of Medical Microbiology

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Comparison of cefoxitin and moxalactam 30 µg disc diffusion methods for detection of methicillin resistance in coagulase-negative staphylococci

Cited by 13 publications

References 9 publications

Correlation of Cefoxitin MICs with the Presence of mecA in Staphylococcus spp

Correlation of Cefoxitin MICs with the Presence of mecA in Staphylococcus spp

Evaluation of Moxalactam with the BD Phoenix System for Detection of Methicillin Resistance in Coagulase-Negative Staphylococci

Improved sensitivity for meticillin resistance detection in coagulase-negative staphylococci by moxalactam antibiotic discs or a cefoxitin investigation zone

Contact Info

Product

Resources

About