Comparison of cauliflower mosaic virus 35S and nopaline synthase promoters in transgenic plants

Sanders, Patricia R.; Winter, Jill; Barnason, Arlene; Rogers, Stephen L.; Fraley, Robert T.

doi:10.1093/nar/15.4.1543

Cited by 213 publications

(121 citation statements)

References 51 publications

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“…In plants containing the fusion genes, GUS activity was localized primarily in cotyledons, with some activity present in the hypocotyl. As controls, we showed that nontransformed seedlings did not stain for GUS activity and, as shown in Figure 3D, that a GUS fusion gene driven by the 355 promoter of cauliflower mosaic virus was expressed differently from the isocitrate lyase and malate synthase fusion genes (Sanders et al, 1987). We also measured GUS activities at different stages of postgerminative growth in plants containing each of the fusion genes and showed that these time courses correlated with modulations in isocitrate lyase and 'malate synthase enzyme activities in B. napus and tobacco (data not shown; Ettinger and Harada, 1990).…”

Section: Regulated Activities Of Lsocitrate Lyase and Malate Synthasementioning

confidence: 82%

Isocitrate Lyase and Malate Synthase Genes from Brassica napus L. Are Active in Pollen

Zhang

Laudencia‐Chingcuanco

Comai

et al. 1994

Plant Physiol.

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To investigate if pollen possesses glyoxysomal function, we analyzed the activities of isocitrate lyase and malate synthase genes. Because the activities of these enzymes were exceedingly low in pollen extracts, we constructed fusion genes encoding j3-glucuronidase (CUS) that are regulated by isocitrate lyase or malate synthase promoters from Brassica napus L. to increase the sensitivity of our assays. Expression of the fusion genes in transgenic tobacco was qualitatively similar to that of the endogenous genes; CUS activity was low in dry seeds, maximal in seedlings, and very low or undetectable in leaves, indicating that the promoters are regulated correctly. We showed that isocitrate lyase and malate synthase genes are active at specific stages of pollen development and that their activities are not enhanced during pollen germination in transgenic tobacco. We also confirmed that the endogenous genes are active by showing that the corresponding mRNAs could be detected in pollen at specific stages of development. The activation of the isocitrate lyase and malate synthase genes suggests that glyoxysomal function is induced during pollen development.Glyoxysomes are specialized peroxisomes that play a pivotal role in the postgerminative growth of oilseed plants (Beevers, 1979;Huang et al., 1983;Trelease and Doman, 1984). These organelles contain the glyoxylate cycle and /3-oxidation enzymes that catalyze the net conversion of fatty acids into succinate and, thus, account for the ability of plants to utilize lipids as a carbon source for carbohydrate synthesis. The carbohydrates generated as a result of these reactions serve as nutrients for seedlings until they become photosynthetically active.Glyoxysomal function is induced at several stages of the plant life cycle when peroxisomes with different metabolic roles are converted into glyoxysomes. The induction of glyoxysomal function is generally monitored using two key glyoxylate cycle enzymes, isocitrate lyase and malate synthase, which are required for glyoxysomal function and are associated exclusively with glyoxysomes (reviewed by Olsen and

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Section: Regulated Activities Of Lsocitrate Lyase and Malate Synthasementioning

confidence: 82%

Isocitrate Lyase and Malate Synthase Genes from Brassica napus L. Are Active in Pollen

Zhang

Laudencia‐Chingcuanco

Comai

et al. 1994

Plant Physiol.

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“…pPRO1-derived plasmids were digested with BglII and EcoRI, and the inserts containing the ORF were purified and ligated into the polylinker region of the plasmid pMON316, digested with the same two restriction enzymes. In this position, the coding sequence is placed between the CaMV 35S promoter and the nopaline synthase 3h untranslated region (Sanders et al, 1987). Leaf disks of N. tabacum cv.…”

Section: Methodsmentioning

confidence: 99%

Transgenic accumulation of two plant virus coat proteins on a single self-processing polypeptide.

Marcos

Beachy²

1997

Journal of General Virology

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An expression cassette based on the highly specific tobacco etch potyvirus (TEV) nuclear inclusion (NIa) proteinase has been developed to produce multiple proteins through the translation of a single selfprocessing polypeptide. Gene constructs encoding TEV NIa, the tobacco mosaic tobamovirus (TMV) coat protein (CP) and the soybean mosaic potyvirus (SMV) CP were used to develop transgenic tobacco plants. Proper processing of the multifunctional polypeptide was demonstrated, leading to accumulation of separate proteins in planta. Moreover, the viral genes expressed in this way were biologically

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“…1B), the sequence was excised from a pUC-type plasmid (13), end-filled with deoxynucleotides by using the Klenow fragment of DNA polymerase I, and then ligated to the intermediate plasmid pMON316 (14) (16). Three independent transformants containing anti-A sequences and one containing anti-B sequences were used for analysis.…”

mentioning

confidence: 99%

Protection against tobacco mosaic virus in transgenic plants that express tobacco mosaic virus antisense RNA.

Powell

Stark

Sanders

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

111

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Transgenic tobacco plants that express RNA sequences complementary to the tobacco mosaic virus (TMV) coat protein (CP) coding sequence with or without the tRNAlike structure at the 3' end of the TMV RNA were produced. Progeny of self-pollinated plants were challenged with TMV to determine their resistance to infection. Plants that expressed RNA sequences complementary to the CP coding region and the 3' untranslated region, including the tRNA-like sequences, were protected from infection by TMV at low levels of inocu- (6) and the expression of the insertion element ISJO (7). In these examples the antisense RNA presumably anneals with its corresponding mRNA and prevents translation (7,8).It has been shown that replication ofthe RNA coliphage SP can be inhibited by antisense RNA by expressing various antisense viral sequences in the host (9, 10). The antisense RNA for the maturase gene was the most effective molecule in these replication inhibition assays, and antisense RNA complementary to sequences at the 3' end of the viral RNA had a lesser effect. Since the latter region contained only the 3' one-third ofthe replicase gene and the 3' noncoding region, the result indicates that antisense RNA may be effective at blocking replication in RNA viruses independent of its ability to block translation. (Fig. 1B)

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Comparison of cauliflower mosaic virus 35S and nopaline synthase promoters in transgenic plants

Cited by 213 publications

References 51 publications

Isocitrate Lyase and Malate Synthase Genes from Brassica napus L. Are Active in Pollen

Isocitrate Lyase and Malate Synthase Genes from Brassica napus L. Are Active in Pollen

Transgenic accumulation of two plant virus coat proteins on a single self-processing polypeptide.

Protection against tobacco mosaic virus in transgenic plants that express tobacco mosaic virus antisense RNA.

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