Comparison of cardioprotective effects of labeled and unlabeled oleanoic acids with new BOPIM dye on primary neonatal rat cardiomyocytes following hypoxia/reoxygenation injury

Wang, Sa; He, Hui; Xiao, Shuzhang; Wang, Junzhi; Bai, Cai-hong; Wei, Na; Zou, Kun

doi:10.1016/j.pharep.2014.03.014

Cited by 6 publications

(3 citation statements)

References 24 publications

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“…17) is a triterpenoid natural compound with a variety of biological activities, such as antioxidant, antihyperlipedemic, antiarrhythmic, and cardiotonic effects. 64 Fluorescent derivative 61 labeled with boron 2-(20-pyridyl)imidazole (BOPIM) was prepared. 65 Evaluation of its cardioprotective effects on primary neonatal rat cardiomyocytes with hypoxia/reoxygenation (H/R) injuries showed significant prevention of H/R-induced cell death.…”

Section: Subcellular Localization and Target Validationmentioning

confidence: 99%

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Natural-product-based fluorescent probes: recent advances and applications

Sung

Lee

2023

RSC Med. Chem.

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Section: Subcellular Localization and Target Validationmentioning

confidence: 99%

“…65 Evaluation of its cardioprotective effects on primary neonatal rat cardiomyocytes with hypoxia/reoxygenation (H/R) injuries showed significant prevention of H/R-induced cell death. 64 Thus, BOPIM-OA 61 is a promising fluorescent probe for investigating the pharmacological mechanisms of natural products.…”

Section: Subcellular Localization and Target Validationmentioning

confidence: 99%

Natural-product-based fluorescent probes: recent advances and applications

Sung

Lee

2023

RSC Med. Chem.

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“…To date, many combination therapies with cardioprotective agents have been proposed to improve DOX treatment efficacy and safety. ACE inhibitors, beta blockers, reduced glutathione, oleanolic and ursolic acids, and antioxidants have been thoroughly studied [27,39,40]. Many of the mentioned drugs have adverse effects and, therefore, the choice of the cardioprotective agent during cancer treatment should be carefully evaluated [41].…”

Section: Discussionmentioning

confidence: 99%

Cinnamic Acid Derivatives as Cardioprotective Agents against Oxidative and Structural Damage Induced by Doxorubicin

Koczurkiewicz-Adamczyk

Klaś

Gunia‐Krzyżak

et al. 2021

IJMS

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Doxorubicin (DOX) is a widely used anticancer drug. However, its clinical use is severely limited due to drug-induced cumulative cardiotoxicity, which leads to progressive cardiomyocyte dysfunction and heart failure. Enormous efforts have been made to identify potential strategies to alleviate DOX-induced cardiotoxicity; however, to date, no universal and highly effective therapy has been introduced. Here we reported that cinnamic acid (CA) derivatives exert a multitarget protective effect against DOX-induced cardiotoxicity. The experiments were performed on rat cardiomyocytes (H9c2) and human induced-pluripotent-stem-cell-derived cardiomyocytes (hiPSC-CMs) as a well-established model for cardiac toxicity assessment. CA derivatives protected cardiomyocytes by ameliorating DOX-induced oxidative stress and viability reduction. Our data indicated that they attenuated the chemotherapeutic’s toxicity by downregulating levels of caspase-3 and -7. Pre-incubation of cardiomyocytes with CA derivatives prevented DOX-induced motility inhibition in a wound-healing assay and limited cytoskeleton rearrangement. Detailed safety analyses—including hepatotoxicity, mutagenic potential, and interaction with the hERG channel—were performed for the most promising compounds. We concluded that CA derivatives show a multidirectional protective effect against DOX-induced cardiotoxicity. The results should encourage further research to elucidate the exact molecular mechanism of the compounds’ activity. The lead structure of the analyzed CA derivatives may serve as a starting point for the development of novel therapeutics to support patients undergoing DOX therapy.

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Anti-proliferative effect of RCE-4 from Reineckia carnea on human cervical cancer HeLa cells by inhibiting the PI3K/Akt/mTOR signaling pathway and NF-κB activation

Bai

Yang

Zou

et al. 2016

Naunyn-Schmiedeberg's Arch Pharmacol

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Cervical cancer is the second leading cause of cancer deaths in women worldwide. In recent years, the studies find that inflammation is a critical component of tumor progression, and the ideal therapeutic methods should be aimed at the inflammation reaction triggers. (1β,3β,5β,25S)-spirostan-1,3-diol1-[α-L-rhamnopyranosyl-(1 → 2)-β-D-xylopyranoside] (RCE-4) was the main active composition of Reineckia carnea (Andr.) Kunth. It significantly induced apoptosis in cervical cancer Caski cells through the mitochondrial pathway in our previous studies; however, its underlying mechanism remains poorly understood. This study aimed to further evaluate the effect of RCE-4 on human cervical cancer HeLa cells. Based on this observation, we investigated the anti-cervical cancer effect of RCE-4 by modulating phosphatidylinositol 3-kinase/protein kinase-B/mammalian target of rapamycin (PI3K/Akt/mTOR) signaling pathway, nuclear factor-kappa B (NF-κB) activation, and inflammation-related key factors in HeLa cells. The results indicated that the HeLa cell was the most sensitive with an IC50 of 7.01 μM; RCE-4 significantly promoted the release of cellular lactate dehydrogenase (LDH); increased DNA fragmentation and apoptosis; reduced PI3K, Akt, mTOR, and NF-κBp65 phosphorylation levels; increased the Bax and cleaved poly (ADP-ribose) polymerase (PARP) protein levels; suppressed Bcl-2 protein expression; elevated the Bax/Bcl-2 expression ratio; and decreased the interleukin-1 beta (IL-1β) and interleukin-6 (IL-6) mRNA expressions in HeLa cells in a concentration-dependent manner. These findings suggest that RCE-4 exerted beneficially anti-cervical cancer effect on HeLa cells, mainly inhibiting PI3K/Akt/mTOR signaling pathway phosphorylation and NF-κB activation, promoting HeLa cell apoptosis. Graphical abstract Anti-tumor effect of RCE-4 on HeLa cells.

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Comparison of cardioprotective effects of labeled and unlabeled oleanoic acids with new BOPIM dye on primary neonatal rat cardiomyocytes following hypoxia/reoxygenation injury

Abstract: BOPIM chromophore is a suitable probe for investigating the pharmacological mechanisms of natural products.

Cited by 6 publications

References 24 publications

Natural-product-based fluorescent probes: recent advances and applications

Natural-product-based fluorescent probes: recent advances and applications

Cinnamic Acid Derivatives as Cardioprotective Agents against Oxidative and Structural Damage Induced by Doxorubicin

Anti-proliferative effect of RCE-4 from Reineckia carnea on human cervical cancer HeLa cells by inhibiting the PI3K/Akt/mTOR signaling pathway and NF-κB activation

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