Comparison of capillary blood versus venous blood samples in the assessment of immunity to measles

Novello, F.; Ridolfi, Barbara; Fiore, Lucia; Buttinelli, Gabriele; Medda, Emanuela; Favero, A.; Marchetti, D.; F, Gaglioppa

doi:10.1016/0166-0934(96)02071-x

Cited by 21 publications

(16 citation statements)

References 6 publications

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“…It has previously been demonstrated that dried blood spots are a feasible alternative to venous blood as a specimen for use in investigations for measles virus antibodies (2,3,5,10). The present study demonstrates for the first time the feasibility of laboratory diagnosis of measles by use of dried blood specimens with a commercial assay.…”

Section: Discussionmentioning

confidence: 69%

“…Several groups have demonstrated a good correlation between serum and peripheral (finger or heel) blood for determination of measles virus antibody titers (2,3,10,15). Our results should therefore be equally applicable to capillary blood specimens.…”

Section: Discussionmentioning

confidence: 94%

“…The use of dried blood spots for the investigation of measles virus hemagglutination inhibition antibodies was reported in the early 1980s (9,15). Since then several other laboratories have demonstrated the feasibility of using dried blood samples in an enzyme immunoassay (EIA) format for the detection of measles virus-specific immunoglobulin G (IgG) (2,10) and IgM (3). In a small study, Novello et al (10) demonstrated the use of dried blood samples with the Dade Behring measles virus-specific IgG EIA for the investigation of immunity to measles virus.…”

mentioning

confidence: 99%

“…However, venipuncture is often resisted by parents and children and may require specially trained staff. Transport of serum and/or plasma specimens and their long-term storage may present logistical problems (10). The use of dried blood spots for the investigation of measles virus hemagglutination inhibition antibodies was reported in the early 1980s (9,15).…”

mentioning

confidence: 99%

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Detection of Measles Virus-Specific Immunoglobulin M in Dried Venous Blood Samples by Using a Commercial Enzyme Immunoassay

et al. 2002

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The optical densities (ODs) of 216 dried venous blood (DVB) samples submitted to the Victorian Infectious Diseases Reference Laboratory as part of enhanced measles surveillance were compared to the ODs of the corresponding serum samples collected at the same time. DVB samples, stored for up to 24 months at 4°C, were tested by the Dade Behring Enzygnost Anti-Measles-Virus/IgM immunoassay. Elution and testing conditions were optimized with the use of spiked DVB samples. The assay showed an overall sensitivity of 90.2% and a specificity of 98.8% for DVB samples compared to the results for serum. When the results were analyzed according to the length of time that the DVB sample had been stored, the assay was 100% sensitive and 97% specific according to the ODs for those samples stored for less than 6 months compared to the results for the corresponding serum samples, with 97.7% agreement between the results for the two sample types. These results demonstrate the potential for the use of DVB samples for the diagnosis of measles in routine diagnostic laboratories.

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Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 94%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Detection of Measles Virus-Specific Immunoglobulin M in Dried Venous Blood Samples by Using a Commercial Enzyme Immunoassay

et al. 2002

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“…The EIA‐related enzyme‐linked immunosorbent assay (ELISA) is an attractive method given the commercial availability of a variety of kits and hence suitability for use in cross‐study and cross‐laboratory comparisons. In DBS, ELISA‐based measurements of measles‐specific IgG have been limited to studies using the Dade Behring Enzygnost ELISA [Novello et al, ; Riddell et al, ; Samoĭlovich et al, ]. There is therefore a need to evaluate additional ELISA kits to avoid dependence upon a single source.…”

Section: Introductionmentioning

confidence: 99%

Use of a commercial ELISA for the detection of measles-specific immunoglobulin G (IgG) in dried blood spots collected from children living in low-resource settings

et al. 2015

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Seroepidemiological monitoring of population immunity to vaccine-preventable diseases is critical to prevent future outbreaks. Dried blood spots (DBS), drops of capillary blood dried on filter paper, are an affordable, minimally invasive alternative to venipuncture for collecting blood in field settings. However, few proven methods exist to analyze DBS for the presence of protective antibodies. This study validates a novel technique for measuring measles-specific immunoglobulin G (IgG) in capillary DBS using a commercial ELISA. The predictive performance of a new method for analyzing DBS was tested by comparing matched serum and DBS samples from 50 children. The accuracy, precision, and reliability of the procedure were evaluated, and the optimal cut points to classify positive and negative samples were determined. The method was then applied to 1,588 DBS collected during a large survey of children in Mexico and Nicaragua. Measles-specific IgG in serum samples were 62% negative, 10% equivocal, and 28% positive. In comparisons with matched serum, DBS results were 100% sensitive and 96 · 8% specific, and agreed in 46 of 50 (92%) cases. The inter-assay and intra-assay coefficients of variation from kit-provided controls were greater than desired (24.8% and 8.4%, respectively). However, in predictive simulations the average misclassification was only 3.9%. Procedures were found to be acceptable to surveyors and participants. Analyzing DBS collected in low-resources settings is a feasible and accurate means of measuring population immunity to measles and should be used to generate objective measures of health status and health system performance.

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Detection of rubella immunoglobulin M from dried venous blood spots using a commercial enzyme immunoassay

Karapanagiotidis

Riddell

Kelly

2005

Diagnostic Microbiology and Infectious Disease

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Comparison of capillary blood versus venous blood samples in the assessment of immunity to measles

Cited by 21 publications

References 6 publications

Detection of Measles Virus-Specific Immunoglobulin M in Dried Venous Blood Samples by Using a Commercial Enzyme Immunoassay

Detection of Measles Virus-Specific Immunoglobulin M in Dried Venous Blood Samples by Using a Commercial Enzyme Immunoassay

Use of a commercial ELISA for the detection of measles-specific immunoglobulin G (IgG) in dried blood spots collected from children living in low-resource settings

Detection of rubella immunoglobulin M from dried venous blood spots using a commercial enzyme immunoassay

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