Comparison of calculated and observed CD spectra of liquid crystalline dispersions formed from double-stranded DNA and from DNA complexes with coloured compounds

Belyakov, V. A.; Orlov, V. P.; Semenov, S.; Skuridin, S. G.; Yevdokimov, Yu. M.

doi:10.1080/02678299608033172

Cited by 55 publications

(42 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1 shows that, in full agreement with the theoretical [13][14][15] and the experimental data [5,[9][10][11], (DNA-DAU) complexes do form two types of cholesteric LC dispersions which differ in the signs of the amplitudes of the intense bands in the CD spectrum. This difference depends upon the way of the LC dispersion preparation.…”

Section: Resultssupporting

confidence: 75%

“…Taking into account the reasons for the appearance of the intense bands in the CD spectra of LC dispersions added with coloured compounds, in particular DAU [15], as well as the dependence of 'extra-intensity' of the CD bands upon DAU and copper concentrations, a comparison of Fig. 2A,B demonstrates that the 'extra-intensity' of the amplitude of the CD band is connected with an increase in concentration of anisotropically oriented DAU molecules.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Formation of polymeric chelate bridges between double‐stranded DNA molecules fixed in spatial structure of liquid‐crystalline dispersions

et al. 1996

View full text Add to dashboard Cite

The formation of cholesteric liquid-crystalline dispersions from DNA-dannomycin complexes in water-salt polyethyleneglycol-contaiuing solutions was investigated. In the case of nonclassical complex formation between DNA and daunomyein (DAU), reactive groups of DAU were used for the formation of polymeric chelate complex with divalent copper ions (-DAUCu-...-Cu-DAU-), located between neighboring double-stranded DNA molecules, fixed in spatial structure of liquid-crystalline dispersions. The formation of polymeric chelate complex does not depend upon the sense of helicoidal twist of DNA cholesteries. A many-fold increase in the CD hand in the DAU absorption region is specific to this process. A reduction of the divalent copper ions as a result of a redox-proeess is accompanied by destroying of structure of polymeric chelate complex between DNA molecules and by disappearance of the abnormal CD band in dannomyein absorption region.

show abstract

Section: Resultssupporting

confidence: 75%

Section: Resultsmentioning

confidence: 99%

Formation of polymeric chelate bridges between double‐stranded DNA molecules fixed in spatial structure of liquid‐crystalline dispersions

et al. 1996

View full text Add to dashboard Cite

show abstract

“…Figure 1 needs a few comments. First of all, the amplitude of the abnormal band in the CD spectrum depends mainly on helical twisting of quasinematic layers of NA in cholesteric structure of PCLCD [11,13] Secondly, the theory [23] predicts that the change in the efficiency of interaction between ds NA molecules in quasinematic layers must be accompanied by changes in the parameters of the spatial helical structure of CLCD particles and in the value of amplitude of an abnormal band in the CD spectra. This means that the decrease in the amplitudes of abnormal bands in the CD spectra (curves 2 -4 and 2 I -5 I ) is connected with the change in the extent of helical twisting of quasinematic layers in structure of PCLCD formed by any type of ds NA molecules, i.e., B-family for DNA and A-family for poly(I) xpoly(C).…”

Section: Resultsmentioning

confidence: 99%

A Dual Effect of Au-Nanoparticles on Nucleic Acid Cholesteric Liquid-Crystalline Particles

Yevdokimov¹,

Skuridin²,

Salyanov³

et al. 2011

JBNB

View full text Add to dashboard Cite

Au-nanoparticles (size about 2 nm, but not 5 or 15 nm) are capable of effectively incorporating into quasinematic layers of particles of cholesteric liquid-crystalline dispersion formed by double-stranded nucleic acid molecules of various families (DNA and poly(I)xpoly(C)). This Au-size-dependent process is accompanied by a decrease in amplitudes of abnormal bands in the CD spectra specific to initial cholesteric liquid-crystalline dispersions and simultaneously by an appearance of plasmon resonance band in visible absorption spectrum. The study of properties of particles of cholesteric liquid-crystalline dispersion treated with Au-nanoparticles by means of various physico-chemical methods demonstrates that incorporation of Au-nanoparticles into quasinematic layers of these particles results in two effects: i) it facilitates reorganization of the spatial cholesteric structure of particles, and ii) it induces the formation of Au-clusters in the content of particles. It is not excluded that these effects represent a possible reason for genotoxicity of Au-nanoparticles.

show abstract

“…This approach was developed for the case of ds DNA LCD particles [55] and recently modified [24,25]. It takes into account both the twisted mode of ds DNA molecule packing and the results above concerning properties of dispersion particles.…”

Section: Constancy Of Ds Dna B-form At Hexagonal Packing Of Moleculesmentioning

confidence: 99%

Re-entrant cholesteric phase in DNA liquid-crystalline dispersion particles

et al. 2016

View full text Add to dashboard Cite

In this research, we observe and rationalize theoretically the transition from hexagonal to cholesteric packing of double-stranded (ds) DNA in dispersion particles. The samples were obtained by phase exclusion of linear ds DNA molecules from water-salt solutions of poly(ethylene glycol)-PEG-with concentrations ranging from 120 mg ml −1 to 300 mg ml −1 . In the range of PEG concentrations from 120 mg ml −1 to 220 mg ml −1 at room temperature, we find ds DNA molecule packing, typical of classical cholesterics. The corresponding parameters for dispersion particles obtained at concentrations greater than 220 mg ml −1 indicate hexagonal packing of the ds DNA molecules. However, slightly counter-intuitively, the cholesteric-like packing reappears upon the heating of dispersions with hexagonal packing of ds DNA molecules. This transition occurs when the PEG concentration is larger than 220 mg ml −1 . The obtained new cholesteric structure differs from the classical cholesterics observed in the PEG concentration range 120-220 mg ml −1 (hence, the term 're-entrant'). Our conclusions are based on the measurements of circular dichroism spectra, X-ray scattering curves and textures of liquid-crystalline phases. We propose a qualitative (similar to the Lindemann criterion for melting of conventional crystals) explanation of this phenomenon in terms of partial melting of so-called quasinematic layers formed by the DNA molecules. The quasinematic layers change their spatial orientation as a result of the competition between the osmotic pressure of the

show abstract

Comparison of calculated and observed CD spectra of liquid crystalline dispersions formed from double-stranded DNA and from DNA complexes with coloured compounds

Cited by 55 publications

References 13 publications

Formation of polymeric chelate bridges between double‐stranded DNA molecules fixed in spatial structure of liquid‐crystalline dispersions

Formation of polymeric chelate bridges between double‐stranded DNA molecules fixed in spatial structure of liquid‐crystalline dispersions

A Dual Effect of Au-Nanoparticles on Nucleic Acid Cholesteric Liquid-Crystalline Particles

Re-entrant cholesteric phase in DNA liquid-crystalline dispersion particles

Contact Info

Product

Resources

About