Comparison of busulfan and total body irradiation conditioning on hematopoietic clonal dynamics following lentiviral gene transfer in rhesus macaques

Abraham, Diana M.; Lozano, Richard J.; Guitart, Xavi; Liang, Jialiu A.; Mortlock, Ryland D.; Espinoza, Diego A.; Fan, Xing; Krouse, Allen E.; Hong, So Gun; Singh, Komudi; Tisdale, John F.; Wu, Chuanfeng; Dunbar, Cynthia E.

doi:10.1016/j.omtm.2022.12.001

Cited by 4 publications

(2 citation statements)

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“…The generation of commercially available cohorts of stem cell humanized mice starts by the treatment of the NSG/NOG mice with busulfan to clear the stem cell niches, alternative to the irradiation, first described at Tisdale’s laboratory ( 85 ). This approach enhances the engraftment of CD34+ human hematopoietic stem cells derived from cord blood, resulting in the development of chimeric human CD45+ (hCD45+) fully matured B and T lymphocytes within 4 to 5 months, all while minimizing associated toxicity ( 85 – 90 ). These engrafted cells differentiate into various immune cell lineages, resulting in a chimeric mouse possessing a human immune system.…”

Section: Humanized Micementioning

confidence: 99%

Animal model considerations for chordoma research: reproducing the tumor microenvironment in vivo with humanized mice

Campilan,

Schroeder,

Sagaityte

et al. 2024

Front. Oncol.

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Animal models have been commonly used in immunotherapy research to study the cell response to external agents and to assess the effectiveness and safety of new therapies. Over the past few decades, immunocompromised (also called immunodeficient) mice allowed researchers to grow human tumor cells without the impact of the host’s immune system. However, while this model is very valuable to understand the tumor biology and to understand the underlying mechanism of immunotherapy, the results may not always directly translate to humans. The tumor microenvironment has significant implications for tumor engraftment, growth, invasion, etc., and the immune system plays a critical role in shaping the tumor microenvironment. Human immunocompetent mice, also named humanized mice, are engineered mice that possess functional human immune cells. This in vivo model can be used to effectively study the effect of the human immune system to a human implanted tumor. Moreover, this can effectively mimic the response to treatment. This section is an overview of the current understanding of the different humanized mice that could be utilized to mimic the tumor microenvironment in chordoma.

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Section: Humanized Micementioning

confidence: 99%

Animal model considerations for chordoma research: reproducing the tumor microenvironment in vivo with humanized mice

Campilan,

Schroeder,

Sagaityte

et al. 2024

Front. Oncol.

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show abstract

“…To achieve successful transplantation, robust stem cell collection methods and pre-transplant conditioning regimens are of utmost importance. 2 , 3 In a recent article published in Molecular Therapy Methods and Clinical Development ( https://doi.org/10.1016/j.omtm.2023.07.006 ), Murray et al. 4 explored the impact of different hematopoietic stem and progenitor cell (HSPC) sources and mobilization regimens on HSPC quality and CRISPR editing efficiency.…”

mentioning

confidence: 99%

Toward effective hematopoietic stem cell gene therapies: Optimized conditioning regimen and stem cell source in harmony

Demirci,

Tisdale

2023

Molecular Therapy - Methods & Clinical Development

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The unknown impact of conditioning on HSC engraftment and clonal dynamics

Radtke

2023

Molecular Therapy - Methods & Clinical Development

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Comparison of busulfan and total body irradiation conditioning on hematopoietic clonal dynamics following lentiviral gene transfer in rhesus macaques

Cited by 4 publications

References 45 publications

Animal model considerations for chordoma research: reproducing the tumor microenvironment in vivo with humanized mice

Animal model considerations for chordoma research: reproducing the tumor microenvironment in vivo with humanized mice

Toward effective hematopoietic stem cell gene therapies: Optimized conditioning regimen and stem cell source in harmony

The unknown impact of conditioning on HSC engraftment and clonal dynamics

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