Comparison of breast milk fatty acid composition from mothers of premature infants of three countries using novel dried milk spot technology

Gao, Chang; Gibson, Robert; McPhee, Andrew J; Zhou, Shao Jia; Collins, Carmel T; Makrides, Maria; Miller, Jacqueline; Liu, Ge

doi:10.1016/j.plefa.2018.08.003

Cited by 5 publications

(4 citation statements)

References 17 publications

(27 reference statements)

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“…PUFAcoat? cards were dried at room temperature for 1 h and stored at room temperature until further analysis ( 27 , 28 ).…”

Section: Methodsmentioning

confidence: 99%

“…Fatty acids were transmethylated to fatty acid methyl esters (FAME) using 2 ml of 1% (v/v) sulphuric acid H 2 SO 4 in anhydrous methanol in a 5 ml sealed vial and heated for 3 h at 70 • C. This procedure allows for fatty acids to be released from structural lipids into a total fatty acid pool. The sequential FAME was extracted into heptanes for further gas chromatography analysis (27,28,30). Sample FAME identification and quantification were achieved by comparing the retention times and peak area values to those of the commercial lipid standards (Nu-Chek-Prep, USA) using the Hewlett-Packard Chemstation data system (27,28,30,31).…”

Section: Pufacoat Milk and Offspring Serum Free Fatty Acid Analysismentioning

confidence: 99%

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Fructose Consumption During Pregnancy Influences Milk Lipid Composition and Offspring Lipid Profiles in Guinea Pigs

et al. 2020

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Excess dietary fructose is a major public health concern ( 1 – 4 ). Evidence shows increased fructose intake can cause insulin resistance, hepatic de novo lipogenesis, hypertriglyceridemia, obesity and non-alcoholic fatty liver disease (NAFLD) ( 5 – 9 ). However, little is known about the effects of fructose during pregnancy and its influence on offspring development and predisposition to later-life disease. To determine whether moderately increased maternal fructose intake could have health consequences on offspring, we have investigated the effects of 10% w/v fructose water intake during preconception and pregnancy. Female Dunkin Hartley guinea pigs were fed a control diet (CD) or fructose diet (FD;10% kcal from fructose) ad-libitum 60 days prior to mating and throughout gestation. Offspring were culled at weaning, day 21 (d21). Compared to CD dams, FD dams had altered glucose metabolism and increased milk free fatty acid content. Matsuda-DeFronzo insulin sensitivity index (M-ISI) from OGTT plasma showed no significant difference in whole-body insulin sensitivity between FD and CD dams 60 days post-dietary intervention and during midgestation. Fetal exposure to increased maternal fructose resulted in offspring with significantly altered serum free fatty acids at days 0, 7, 14, and 21 [including pentadecanoic acid (15:0), dma16:0, margaric acid (17:0) palmitoleic acid, total omega-7 and total saturates], increased levels of uric acid and triglycerides were also observed at d21. We have demonstrated that increased fructose intake during pregnancy can cause significant changes in maternal metabolic function and milk composition, which alters offspring metabolism. Taken together, these changes in pregnancy outcomes and feto-maternal condition may underlie their offspring's predisposition to metabolic dysfunction during later-life.

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“…PUFAcoat? cards were dried at room temperature for 1 h and stored at room temperature until further analysis ( 27 , 28 ).…”

Section: Methodsmentioning

confidence: 99%

Section: Pufacoat Milk and Offspring Serum Free Fatty Acid Analysismentioning

confidence: 99%

Fructose Consumption During Pregnancy Influences Milk Lipid Composition and Offspring Lipid Profiles in Guinea Pigs

et al. 2020

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“…Total fatty acids in the blood and breast milk were measured using dried blood and milk spot methods [17,20]. Briefly, the dried blood/ milk spot was added to 2 mL of 1% (v/v) H 2 SO 4 (18 M AR grade, BDH, Sussex, UK) in anhydrous methanol (Merck, Darmstadt, Germany) in a 6 mL scintillation vial (Wheaton, Millville, USA), and heated at 70°C for 3 h. The resultant fatty acid methyl esters (FAME) were extracted into heptanes (Merck, Darmstadt, Germany).…”

Section: Total Fatty Acid Analysismentioning

confidence: 99%

Intravenous fat induces changes in PUFA and their bioactive metabolites: Comparison between Japanese and Australian preterm infants

Suganuma

McPhee

Collins

et al. 2020

Prostaglandins, Leukotrienes and Essential Fatty Acids

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Oxylipins are biologically active signaling molecules that initiate and resolve inflammation; they are synthesized by oxidation of polyunsaturated fatty acids (PUFAs) and reflect PUFA intake and status. The PUFA intake in preterm infants differs between countries because of the type of lipid emulsions used and the PUFA content of breast milk. We compared total blood PUFA, free PUFA and their oxylipin levels in dried whole blood samples from preterm infants born in Australia and Japan. Methods: We enrolled 30 and 14 preterm infants born less than 31 weeks' gestation, from Adelaide and Japan respectively. Blood samples were obtained from cord blood, and on postnatal days 4, 7, 14 and 28. Total PUFAs were measured using gas chromatography, while free fatty acids and oxylipins were screened using ultra highperformance liquid chromatography mass spectroscopy. Results: Differences in the levels of blood PUFA between the centres were found which were in line with the timing and type of lipid emulsion administration. Significant differences in longitudinal levels were seen more often in free PUFA and their oxylipins than in total blood PUFA. This was particularly true for AA and DHA. In contrast, differences in the levels could be seen in total blood EPA, as well as in free EPA and its oxylipins. Further, levels of many free PUFA and their oxylipins were higher in Japanese infants than in Australian infants. Conclusion: Differences in total and free fatty acids and unesterified oxylipins, were observed during the first weeks of life and between preterm infants born in Australia and Japan, which were likely a reflection of the type of lipid emulsion and timing of administration. The clinical significance of these changes remains to be explored. including the omega-6 linoleic acid (LA) and the omega-3 alpha linolenic acid (ALA), as well as their long chain PUFA metabolites arachidonic acid (AA-an omega-6 derivative), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), both omega-3 derivatives. The majority of fats in the circulation, whether derived from tissue sources, the diet or administered intravenously, exist esterified into triglycerides, phospholipids or cholesteryl esters. In response to various

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“…Hence, dried matrix (i.e., blood, urine, milk) spots on paper is a more attractive sample type because of low sample volume required, ease of collection, room-temperature storage, and low-cost postal shipping. Although dried blood spots (DBSs) have been used in a wide range of research including metabolomics studies, 16 few studies explored the use of different dried milk spots systems, [17][18][19] and none for veterinary or agricultural applications. These established dried milk spot (DMS) systems studied human breast milk and often require pre-treatment of papers using different protocols which can introduce extra inconsistencies between studies.…”

Section: Introductionmentioning

confidence: 99%

Metabolic alterations in dairy cattle with lameness revealed by untargeted metabolomics of dried milk spots using direct infusion-tandem mass spectrometry and the triangulation of multiple machine learning models

Cardoso

Hyde

et al. 2022

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Comparison of breast milk fatty acid composition from mothers of premature infants of three countries using novel dried milk spot technology

Abstract: Comparison of breast milk fatty acid composition from mothers of premature infants of three countries using novel dried milk spot technology ,

Cited by 5 publications

References 17 publications

Fructose Consumption During Pregnancy Influences Milk Lipid Composition and Offspring Lipid Profiles in Guinea Pigs

Fructose Consumption During Pregnancy Influences Milk Lipid Composition and Offspring Lipid Profiles in Guinea Pigs

Intravenous fat induces changes in PUFA and their bioactive metabolites: Comparison between Japanese and Australian preterm infants

Metabolic alterations in dairy cattle with lameness revealed by untargeted metabolomics of dried milk spots using direct infusion-tandem mass spectrometry and the triangulation of multiple machine learning models

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