Comparison of Box-Behnken, Face Central Composite and Full Factorial Designs in Optimization of Hempseed Oil Extraction by n-Hexane: a Case Study

Stamenković, Olivera S.; Kostić, Milan D.; Radosavljević, Dragana; Veljković, Vlada B.

doi:10.3311/ppch.11448

Cited by 31 publications

(14 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hemp ( Cannabis sativa L.) is a herbaceous plant that belongs to the Cannabaceae family, and is widely distributed all over the world 1 . It has many industrial applications including textile, paper, food, medicinal, pharmaceutical, cosmetic, and others 2 . The genus Cannabis has been divided into three main species: (i) fibre‐type ( C. sativa L.) (ii) drug‐type ( C. indica Lam.…”

Section: Introductionmentioning

confidence: 99%

Development of ANN models based on combined UV‐vis‐NIR spectra for rapid quantification of physical and chemical properties of industrial hemp extracts

Valinger

Jurina

Šain

et al. 2020

Phytochemical Analysis

View full text Add to dashboard Cite

Objectives The aim of this study was to develop artificial neural network (ANNs) models for prediction of physical (total dissolved solids, extraction yield) and chemical (total polyphenolic content, antioxidant activity) properties of industrial hemp extracts, prepared by two different extraction methods (solid‐liquid extraction and microwave‐assisted extraction) based on combined UV‐VIS‐NIR spectra. Spectral data were gathered for 46 samples per extraction method. Results The PCA analysis ensured efficient separation of the samples based on the amount of ethanol in extraction solvent using NIR spectra for both conventional and microwave‐assisted extraction. Conclusions Results showed that reliable ANN models (R2>0.7000) for describing physical, chemical, and simultaneously physical and chemical characteristics can be developed based on combined UV‐VIS‐NIR spectra of industrial hemp extracts without spectra pre‐processing.

show abstract

Section: Introductionmentioning

confidence: 99%

Development of ANN models based on combined UV‐vis‐NIR spectra for rapid quantification of physical and chemical properties of industrial hemp extracts

Valinger

Jurina

Šain

et al. 2020

Phytochemical Analysis

View full text Add to dashboard Cite

show abstract

“…Similarly, the observation for the adjusted R 2 values for the four models was close to 1. R 2 and adjusted R 2 values were in good agreement, with an approximately 95% confidence level, implying that the experimental and predicted efficiencies were not much different [46,48]. Additionally, the predicted R 2 values of 0.8293, 0.8237, 0.8999, and 0.9202 for PLA/clay composites loaded with NaOH-modified K85 rice husks, Mg(OH) 2modified K85 rice husks, NaOH-modified K98 rice husks, and Mg(OH) 2 -modified K98 rice husks, respectively, were in reasonable agreement with adjusted R 2 values (respective differences were less than 0.2) [31,46].…”

Section: Analysis Of Variance For Model Statistical Significancementioning

confidence: 68%

Optimization of tensile strength of PLA/clay/rice husk composites using Box-Behnken design

Yiga

Lubwama

Pagel

et al. 2021

Biomass Conv. Bioref.

View full text Add to dashboard Cite

It is extremely important to save costs and time while enhancing accuracy in experimentation. However, no study has utilized response surface methodology (RSM) to obtain the effects of independent parameters on properties of PLA/clay/rice husk composites. This study focused on optimization of tensile strength of fiber-reinforced polylactic acid (PLA) composites. RSM using Box-Behnken design (BBD) was used to determine optimum blending parameters of the developed composites. Fiber-reinforced PLA composites were prepared using compression molding. Rice husk fiber and clay filler were used to enhance tensile properties of PLA. Five factors, namely, clay filler loading (1 − 5 wt.%), rice husk fiber loading (10 − 30 wt.%), alkali concentration (0 − 4 wt.%), rice husk variety (K85, K98), and alkali type (NaOH, Mg(OH)2) were varied with 68 individual experiments. Tensile tests were carried out according to ASTM D638 standards. ANOVA results revealed that the quadratic models best fit the tensile strength response, with filler loading and fiber loading factors as the most significant model terms. Interaction effects were more predominant than linear and quadratic effects. The developed models used to determine maximum tensile strengths of PLA/clay/rice husk composites were in close agreement with experimental findings (R2 values of 0.9635, 0.9624, 0.9789, and 0.9731 for NaOH-modified K85 rice husks, Mg(OH)2-modified K85 rice husks, NaOH-modified K98 rice husks, and Mg(OH)2-modified K98 rice husks respectively). Individual optimal conditions were used to predict maximum tensile strengths in each set of developed composites. The predicted tensile strengths were 32.09 MPa, 33.69 MPa, 32.47 MPa, and 32.75 MPa for PLA/clay composites loaded with NaOH-modified K85 rice husks, Mg(OH)2-modified K85 rice husks, NaOH-modified K98 rice husks, and Mg(OH)2-modified K98 rice husks, respectively, which were very close to the obtained experimental values of 31.73 MPa, 33.06 MPa, 32.02 MPa, and 31.86 MPa respectively.

show abstract

“…Thus, all design points fall within their safe operating zone. Box-Behnken designs factors are not set at their high levels at the same time (Stamenković et al 2018). Hence, Box-Behnken design is easy to predict the lower and upper limits at 3 level point, and this method was used for the melanin optimization in this study.…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility and radioprotection by newly characterized melanin pigment and its production from Dietzia schimae NM3 in optimized whey medium by response surface methodology

Eskandari

Etemadifar

2021

Ann Microbiol

View full text Add to dashboard Cite

Purpose To characterize and optimize the productivity of melanin using an extremotolerant actinobacterium, Dietzia schimae NM3, for the first time. Methods An extracellular brown pigment produced by D. schimae NM3 in the nutrient broth and cheese whey medium by adding L-tyrosine. The extracted melanin was analyzed by UV-visible, HPLC, and FTIR assays. The radical scavenging activity (by DPPH) and sun protection factor (SPF) of the extracted melanin were measured. The melanin cytotoxicity was assayed by MTT and chromate biosorption was measured by atomic absorption spectroscopy. Finally, melanin production by D. schimae NM3 was optimized by response surface methodology (RSM) using Box-Behnken design in the whey medium. Result The purified melanin showed similar peak to the standard melanin (SIGMA) at 3.5 min in HPLC, and C=O bands, NH2, CH, C-N, and aromatic groups by FTIR. The radical scavenging activity (by DPPH) and SPF of the extracted melanin were obtained 188.9% and 20.22, respectively. Using MTT assay, the melanin revealed non-toxic effect on the normal human fibroblast (HFB) cell culture. The melanin yield 790 mg l−1, and tyrosinase activity 3400 U ml−1 were obtained in the medium contained whey powder [5% (w v−1)], L-tyrosine 2.5 g l−1, CuSO4 0.013 g l−1, and pH 10.5, incubated at 32 °C for 3 days. The ANOVA results indicated significant P-value, model F-value, and probability, with insignificant lack of fit. After optimization with mono-factors, the nutrient broth came up with melanin yield as 1.2 g l−1 and tyrosinase activity as 4040 U ml−1. Conclusion This is the first report of melanin production by D. schimae NM3 and this natural melanin showed valuable biological properties such as high antioxidant activity and radioprotection (SPF) and the biocompatibility to human cell line.

show abstract

Comparison of Box-Behnken, Face Central Composite and Full Factorial Designs in Optimization of Hempseed Oil Extraction by n-Hexane: a Case Study

Abstract: Statistical multivariate methods like Box-Behnken, face central composite and full factorial designs (BBD, FCCD and FFD, respectively)

Cited by 31 publications

References 29 publications

Development of ANN models based on combined UV‐vis‐NIR spectra for rapid quantification of physical and chemical properties of industrial hemp extracts

Development of ANN models based on combined UV‐vis‐NIR spectra for rapid quantification of physical and chemical properties of industrial hemp extracts

Optimization of tensile strength of PLA/clay/rice husk composites using Box-Behnken design

Biocompatibility and radioprotection by newly characterized melanin pigment and its production from Dietzia schimae NM3 in optimized whey medium by response surface methodology

Contact Info

Product

Resources

About