Comparison of Bone Healing by Demineralized Bone Matrix and Autogenous Cancellous Bone in Horses

Kawcak, Chris E.; Trotter, Gayle W.; Powers, Barbara E.; Park, R D; Turner, A. Simon

doi:10.1053/jvet.2000.5601

Cited by 30 publications

(27 citation statements)

References 31 publications

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“…Better results with autogenous bone graft are credited to its lack of immunogenicity, minimum inflammatory reaction and easy revascularization, great quantity of viable cells and high potential of osteoconduction and osteoinduction (Piattelli et al, 1996;Rabie and Lie Ken Jie, 1996;Kawcak et al, 2000;Rabie and Chay, 2000). The present results showed favorable influence on the use of autogenous graft, confirming the superiority of autogenous grafts as reconstructive materials.…”

Section: Discussionsupporting

confidence: 73%

Histological and histomorphometric analyses of calcium phosphate cement in rabbit calvaria☆

Cavalcanti

Pereira

Mazzonetto

et al. 2008

Journal of Cranio-Maxillofacial Surgery

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Section: Discussionsupporting

confidence: 73%

Histological and histomorphometric analyses of calcium phosphate cement in rabbit calvaria☆

Cavalcanti

Pereira

Mazzonetto

et al. 2008

Journal of Cranio-Maxillofacial Surgery

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“…By using an appropriate matrix, it may be possible to construct a single unit that is capable of repairing both the chondral and subchondral aspects of the defect (10). The BMG used in this study was reported to be a suitable scaffold supporting the growth of osteochondral tissue (11). The widely available natural polymer, fibrin, has been reported to be an ideal cell carrier (12).…”

Section: Discussionmentioning

confidence: 92%

Cartilage Tissue Engineering With Demineralized Bone Matrix Gelatin and Fibrin Glue Hybrid Scaffold: An In Vitro Study

Wang

Zhou

et al. 2010

Artificial Organs

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To develop a cartilage-like tissue with hybrid scaffolds of demineralized bone matrix gelatin (BMG) and fibrin, rabbit chondrocytes were cultured on hybrid fibrin/BMG scaffolds in vitro. BMG scaffolds were carefully soaked in a chondrocyte-fibrin suspension, which was polymerized by submerging the constructs into thrombin-calcium chloride solution. Engineered cartilage-like tissue grown on the scaffolds was characterized by histology, immunolocalization, scanning electron microscopy, biochemical assays, and analysis of gene expression at different time points of the in vitro culture. The presence of proteoglycan in the fibrin/BMG hybrid constructs was confirmed by positive toluidine blue and alcian blue staining. Collagen type II exhibited intense immunopositivity at the pericellular matrices. Chondrogenic properties were further demonstrated by the expression of gene-encoded cartilage-specific markers, collagen type II, and aggrecan core protein. The glycosaminoglycan production and hydroxyproline content of tissue grown on the fibrin/BMG hybrid scaffolds were higher than that of the BMG group. In conclusion, the fibrin/BMG hybrid scaffolds may serve as a potential cell delivery vehicle and a structural basis for cartilage tissue engineering.

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“…In these cases, species-specific DBM is required. 42,46 Commonly, for osteoinductivity assays, the DBM is resident in the animal for at least 28 days. 45 After explantation, histomorphometry is performed on stained, embedded, decalcified sections.…”

Section: Demineralized Bone Matrixmentioning

confidence: 99%

Allograft and Alloplastic Bone Substitutes: A Review of Science and Technology For the Craniomaxillofacial Surgeon

Eppley

Pietrzak²,

Blanton³

2005

Journal of Craniofacial Surgery

196

108

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Bone healing is a complex and multifactorial process. As such, there are numerous steps in the process to which intervention can be directed. This has given rise to many bone graft technologies that have been used to regenerate bone, creating, perhaps, a bewildering array of options. The options that surgeons have the most familiarity with are the ones that have been available the longest (i.e., autograft and allograft). Although useful for the widest spectrum of clinical applications, limitations of these grafts has prompted the development of new materials. Demineralized bone matrix formulations and synthetic ceramic materials are now being used with greater frequency. These biomaterials have demonstrated their usefulness in facial plastic and reconstructive surgery with their ability to augment and replace portions of the craniofacial skeleton. The purpose of this article is to describe and discuss the allograft and alloplastic bone grafting technologies so that the reader can consider each in the context of the others and gain a better appreciation for how each fits into the universe of existing and emerging treatments for bone regeneration.

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Comparison of Bone Healing by Demineralized Bone Matrix and Autogenous Cancellous Bone in Horses

Abstract: Particles of 2 to 4 mm DBM should not be used as an aid to fracture repair because particles of this size interfere with normal mineralization. However, our model of nonunion fracture healing may be useful in future studies.

Cited by 30 publications

References 31 publications

Histological and histomorphometric analyses of calcium phosphate cement in rabbit calvaria☆

Histological and histomorphometric analyses of calcium phosphate cement in rabbit calvaria☆

Cartilage Tissue Engineering With Demineralized Bone Matrix Gelatin and Fibrin Glue Hybrid Scaffold: An In Vitro Study

Allograft and Alloplastic Bone Substitutes: A Review of Science and Technology For the Craniomaxillofacial Surgeon

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