Different types of training methods can affect children's physical fitness as well as performance in different ways. The aim of this study was to compare the effect of jump rope training on some of the physical and motor fitness factors between male and female primary school students. Forty-three male and female students from primary school volunteered. The students participated in a jump rope training program for 6 weeks (3 sessions per week). Before and after the training periods physical and motor fitness tests including cardiovascular endurance, agility, speed, legs explosive power, hands muscle endurance, hands power, static and dynamic balance were taken from the participants. The data were analyzed using the ANCOVA and dependent T-tests at a significance level of P<0.05. The results showed no significant difference between male and female students in the indices of cardiovascular endurance, speed, hands muscular endurance, hands muscle power, legs explosive power, static and dynamic balance (P>0.05), while the agility index was improved in male students. It is suggested that male and female primary school students use Jump Rope trainings to improve their physical fitness and motor factors.