Comparison of birth weight and growth characteristics of bovine calves produced by nuclear transfer (cloning), embryo transfer and natural mating

Wilson, J. Frank; Williams, John D.; Bondioli, K. R.; Looney, C.R.; Westhusin, Mark; McCalla, D.F

doi:10.1016/0378-4320(94)01353-n

Cited by 176 publications

(85 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thompson et al (1995) (Wilmut and Sales, 1981) or to the oviducts of progesterone treated ewes (Kleemann et al, 1994) causes enhanced fetal growth. In cattle, in vitro procedures also affect fetal growth (Behboodi et al, 1995;Farin and Farin, 1995), but not duration of gestation (Behboodi et al, 1995), while cloning by nucleus transfer affects both (Willadsen et al, 1991;Wilson et al, 1995). The numbers of lambs and variation in birth weights in this study do not allow further conclusions regarding the possible relations between the type of manipulation, birth weight and duration of gestation.…”

Section: Discussionmentioning

confidence: 56%

See 1 more Smart Citation

Embryo viability, duration of gestation and birth weight in sheep after transfer of in vitro matured and in vitro fertilized zygotes cultured in vitro or in vivo

Holm¹,

Walker²,

Seamark³

1996

Reproduction

130

View full text Add to dashboard Cite

The influence of various in vitro procedures on embryo survival and the production of normal offspring was investigated in sheep. Zygotes with both in vivo cultured IVM\p=n-\IVF zygotes and control zygotes 25\p=n-\35% versus 51\p=n-\60%, respectively, P < 0.05). Day 6.5 IVM\p=n-\IVFmorulae had a lower survival rate than did control morulae regardless of culture treatment (P < 0.05), while survival rates of day 6.5 IVM\p=n-\IVF blastocysts cultured in vivo did not differ from those of control blastocysts (P > 0.1). Both the gestation period and birth weight of IVM\p=n-\IVFlambs were increased when compared with controls, the former significantly in all groups (154.0\p=n-\154.9days versus 150.6 days; P < 0.01), while the latter increase was on the borderline of significance (4\ m=. \ 5\ p=n-\ 4\ m=. \ 8 kg versus 4.0 kg; 0.01 \m=le\P \m=le\0.1, respectively) and dependent on the prolongation of the gestation period. It is concluded that in vitro maturation and fertilization compromise subsequent embryonic and fetal development in sheep irrespective of the subsequent in vivo or in vitro culture treatment. Subjecting IVM\p=n-\IVF zygotes to in vivo culture for 6.5 days minimizes only some of these effects, thus leading to the aberrant production of some offspring.

show abstract

Section: Discussionmentioning

confidence: 56%

“…This finding is supported by studies in cattle (Behboodi et al, 1995;Farin and Farin, 1995 (Willadsen et al, 1991;Wilson et al, 1995 (Behboodi et al, 1995). Thompson et al (1995) (Wilmut and Sales, 1981) or to the oviducts of progesterone treated ewes (Kleemann et al, 1994) causes enhanced fetal growth.…”

Section: Discussionmentioning

confidence: 66%

Embryo viability, duration of gestation and birth weight in sheep after transfer of in vitro matured and in vitro fertilized zygotes cultured in vitro or in vivo

Holm¹,

Walker²,

Seamark³

1996

Reproduction

130

View full text Add to dashboard Cite

show abstract

“…Morphologically, they showed successful reprogramming without any defects at least until the pre-implantation stage. However, it is widely acknowledged that nuclear transfer, even with embryonic blastomeres, results in increased rates of abortion throughout pregnancy, high birth weight, perinatal deaths, and poor adaptation to extra-uterine life [2,4,11]. These effects appear more extreme with somatic cell nuclear transfer [1, Second, due to the relatively small size of HanWoo calves, the chance of dystocia by large calf derived from in vitro produced embryos can be reduced, even in twin pregnancies [5].…”

Section: Discussionmentioning

confidence: 99%

Production of HanWoo(Bos taurus coreanae) Fetuses Following Interbreed Somatic Cell Nuclear Transfer.

Roh

Yoon

2001

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. The possibility of producing HanWoo (Bos taurus coreanae) calves from transferable bovine embryos, obtained by interbreed nuclear transfer using Holstein cytoplasts and surrogates, was investigated. As donor nuclei, HanWoo fetal fibroblasts were used. Cells were induced into quiescence by serum deprivation for 4-7 days before nuclear transfer. In vitro matured Holstein oocytes were enucleated, and single donor cell was placed into the perivitelline space of enucleated oocyte. After reconstruction, the embryos were fused, activated and cultured. On day 7, the embryos that developed to the blastocyst stages were transferred into Holstein recipient cows on day 6 to 7 of estrous cycle (estrus=0). The reconstructed embryos were successfully fused (58.8%; 47/80), cleaved (91.5%; 43/47), and developed to blastocysts (29.8%; 14/47). Eleven blastocysts were transferred into 5 Holstein recipient cows. Two recipients were pregnant, confirmed by ultrasonography at day 60 of gestation. But, one of them was opened between on day 80 to 100 of pregnancy, and the other had a stillbirth on day 255. The stillborn calf was physically normal, and we couldn't find any evidence of anomaly. These results show that cells derived from HanWoo somatic cell lines can be reprogrammed by interbreed nuclear transfer and develop subsequently in vivo as well as in vitro. KEY WORDS: HanWoo (Bos taurus coreanae), interbreed, nuclear transfer.J. Vet. Med. Sci. 63(9): 945-948, 2001 Production of cloned animal by somatic cell nuclear transfer has recently been developed. The birth of live offspring derived from embryos reconstructed with somatic cells have been reported in several species; sheep [10], cattle [1,3,8], and mouse [7].Animal cloning technology can be used to preserve endangered mammalian species by interbreed or interspecies nuclear transfer. The success of interbreed nuclear transfer was reported in last surviving cow of the Enderby island cattle breed [9]. HanWoo (Bos taurus coreanae) inhabits only in the region of Korea peninsula and becomes endangered animal in the bovine species. To produce HanWoo more efficiently, we developed in vitro production and embryo transfer system and produced HanWoo twin calves from the Holstein recipient cows [5]. Due to the relatively small size of HanWoo calves, we could obtain twins easily without dystocia or other problems during the pregnancy.In this study, we used Holstein recipient cytoplast for the production of HanWoo nuclear transfer embryos, and transfer them into Holstein cows to obtain cloned HanWoo offspring. MATERIALS AND METHODS Preparation of fetal fibroblasts:Fibroblast cells were obtained from HanWoo conceptuses on Day 50 to 60 of pregnancy. The head, extremities and internal organs were removed before tissues were cut into small pieces, and cells were dispersed by exposure to 0.25% (w/v) trypsin solution (Gibco BRL, Grand Island, NY). Cells were cultured in Dulbeco's minimum essential medium (Gibco BRL) supplemented with 10% fetal bovine serum (FBS, Gibco BRL). For each p...

show abstract

“…If the higher variation in the weight of newborn calves derived from in vitro fertilisation (IVF) may be explained by an incomplete maturation of oocytes at the time of fertilisation, it is also possible that MO concerns smaller fol-Acta Veterinaria Hungarica 51, 2003 licles yielding incompetent or partially competent oocytes. Alternatively, the higher variability in the weight of calves originating from cloning (Wilson et al, 1995) may be due to the in vitro exposure of the gametes and (or) the embryos. These questions are worthy of an experimental confirmation in a contemporary comparison of ongoing pregnancies after transfer of embryos produced in normal non-stimulated cycle and after superovulation induction, using of course the same combination of donor, bull and recipient because these parameters influence the endocrinological pattern of pregnancy (Guilbault et al, 1990).…”

Section: In Vitro Manipulations: Possible Consequences On Gene Expresmentioning

confidence: 99%

From the germinal cells to the newborn animal: The transmission of genes and life through the generations

Drion

Szenci

Ectors

et al. 2003

Acta Veterinaria Hungarica

View full text Add to dashboard Cite

The technology of reproduction progressed considerably during the last decade, leading to a certain availability of in vitro methods for fertilisation, oocyte maturation and embryo culture. The most spectacular manipulations are cloning and transgenesis. This review focuses on the early appearance of germinal cell precursors and the long-standing fate of gametes in mammals. The evident complexity and long-term programming of events in gametes and early embryos explain part of the difficulties encountered during the development of in vitro and in vivo methods such as multiple ovulation and embryo transfer (MOET), oestrus synchronisation, ovulation induction, superovulation, in vitro maturation and fertilisation, cryopreservation, transgenesis, nuclear transfer and cloning) and the occurrence of unexpected alterations of development, e.g. embryonic or fetal mortality, large-weight newborn syndrome and other dysregulations in imprinting or DNA transmission.

show abstract

Comparison of birth weight and growth characteristics of bovine calves produced by nuclear transfer (cloning), embryo transfer and natural mating

Cited by 176 publications

References 23 publications

Embryo viability, duration of gestation and birth weight in sheep after transfer of in vitro matured and in vitro fertilized zygotes cultured in vitro or in vivo

Embryo viability, duration of gestation and birth weight in sheep after transfer of in vitro matured and in vitro fertilized zygotes cultured in vitro or in vivo

Production of HanWoo(Bos taurus coreanae) Fetuses Following Interbreed Somatic Cell Nuclear Transfer.

From the germinal cells to the newborn animal: The transmission of genes and life through the generations

Contact Info

Product

Resources

About