Comparison of biological features between severely immuno-deficient NOD/Shi-<i>scid Il2rg^null</i> and NOD/LtSz-<i>scid Il2rg^null</i> mice

Abstract

Biological background data up to 11 weeks of age and tumorigenic susceptibility to xenotransplantation with HeLa cells were compared between severely immuno-deficient NOG and NSG mice. The body weight was lower in NOG mice than in NSG mice. Severe depletion of peripheral blood lymphocytes and lymphoid hypoplasia that are well-known characteristics of these mice were equally observed. No lymphoproliferative lesions developed in any mouse of either strain. The occurrence of ectopic exocrine gland and cyst was a common finding in the thymus of both strains. In addition, minimal spongiotic change was observed in the medulla oblongata and spinal cord in both strains, and its incidence in female NOG mice was a little higher than that in NSG mice. In the adrenal, subcapsular cell hyperplasia that is known as an age-related change in non-genetically modified mice developed earlier and its incidence was higher in NSG mice than in NOG mice. The development of female genital organs of NOG mice was slightly retarded in comparison with that of NSG mice. To evaluate tumorigenic susceptibility to xenotransplantation, female mice were implanted in the dorsal subcutis with 1×103 to 1×106 cells/head of HeLa cells, and were checked up to 16 weeks after implantation. As a result, there was no significant strain difference on tumor formation rate and tumor volume. In conclusion, the present study clearly demonstrated that NOG and NSG mice showed no distinct strain differences in either biological features or biological disadvantages.

Application of Highly Immunocompromised Mice for the Establishment of Patient-Derived Xenograft (PDX) Models

“…NOG mice have a NOD/ShiJic-Prkdc scid background with partial deficiency of IL2Rγ [21], whereas NSG mice have a NOD/ShiSzJ-Prkdc scid background with complete deficiency of IL2Rγ [10]. The NSG mice acquire higher engraftment capacity of cord-blood-derived CD34 + cells [24] and higher body weights [25], but these differences do not appear to affect the PDX transplantation efficiency [25]. Moreover, signal regulatory protein alpha (Sirpα) polymorphism in NOD strains provides superior opportunities for human cell engraftment because SIRPα interacts with human CD47 [26] and suppresses macrophage-mediated phagocytosis, or contributes to the so-called “don’t eat me” signal (Figure 1) [27,28].…”

Prolongedin-vivotracking of vitreous fluid and its early diagnostic imaging biomarkers for cancer growth

“…While innate immunity remains impaired in both genotypes, NK activity in NSGS remains impaired, whereas, in Nu/Nu, NK cell density increases with age 69,70 . We note that these findings are not specifically estimated for vitreous humour.…”

Spontaneous early-onset neurodegeneration in the brainstem and spinal cord of NSG, NOG, and NXG mice

“…12,25 Even though the 3 mouse strains feature distinct Il2rg targeted mutations and different NOD backgrounds, these models are considered equivalent in terms of the overall biological/ physiological characteristics and experimental applications. 22 The study of naturally occurring and experimentally induced lesions affecting NSG and NOG has been the subject of intense investigation by veterinary pathologists. 2,29,34,40,41 Notably, the increased prevalence and severity of inclusion body nephropathy in NSG mice have led to the discovery of the novel mouse kidney parvovirus.…”

“… 12 , 25 Even though the 3 mouse strains feature distinct Il2rg targeted mutations and different NOD backgrounds, these models are considered equivalent in terms of the overall biological/physiological characteristics and experimental applications. 22 …”