Comparison of biological and transcriptomic effects of conventional cigarette and electronic cigarette smoke exposure at toxicological dose in BEAS-2B cells

Wang, Lilan; Wang, Yao; Chen, Jianwen; Yang, Xiangming; Jiang, Xingtao; Liu, Peiqing; Li, Min

doi:10.1016/j.ecoenv.2021.112472

Cited by 17 publications

(11 citation statements)

References 36 publications

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“…Likewise, our acellular ROS data suggest that adding WS-23 to nicotine-containing e-liquid base leads to noteworthy changes in generated acellular ROS levels. Our findings are similar to that of previous studies showing that treatments with e-liquids induce significant levels of ROS production in BEAS-2B cells when compared to untreated cells [29] . Regarding our understanding of the potentially harmful effects of WS-23, our data seems to suggest that WS-23 itself has a limited impact in altering e-cig-generated acellular ROS levels as well as a limited effect on modifying the levels of ROS generated by BEAS-2B cells.…”

Section: Discussionsupporting

confidence: 92%

“…Omaiye et al [33] used Lactase Dehydrogenase (LDH), Neutral Red Uptake (NRU), and MTT (3-(4,5-dimethylthiazol-2-yl)− 2,5-diphenyltetrazolium bromide) tetrazolium reduction assays to assess the role of exposure to aerosols generated by e-liquids containing WS-3 and WS-23 have an effect in inducing cytotoxicity in human bronchial epithelial cells [33] . Similar to this study [29] , our study involved analyzing the cellular responses of BEAS-2B cells to e-liquids containing either WS-3 or WS-23, and showed some cytotoxic responses when WS-3 and WS-23 combined with e-cig nicotine. However, n contrast to Omaiye et al [33] , which assessed the cytotoxicity induced by different treatments, our study assessed differences in ROS production.…”

Section: Discussionmentioning

confidence: 87%

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The role of synthetic coolants, WS-3 and WS-23, in modulating E-cigarette-induced reactive oxygen species (ROS) in lung epithelial cells

et al. 2022

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 87%

The role of synthetic coolants, WS-3 and WS-23, in modulating E-cigarette-induced reactive oxygen species (ROS) in lung epithelial cells

et al. 2022

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“…Regarding limitations in our study, our study did not include the treatment of airway epithelial cells with aerosolized e-liquids. Previous studies have shown that treatments with e-liquids induce significant levels of ROS production in Human Bronchial Epithelial cells (BEAS-2B) (Wang, Wang et al 2021). Epithelial cells lining the airways are the first structural cell targets of any inhaled substances (Hiemstra, Tetley et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Synthetic Coolant WS-23 increases E-Cigarette Generated Aerosolized Acellular Reactive Oxygen Species (ROS) Levels

Yogeswaran

Manevski

Chand

et al. 2022

Preprint

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There has been a substantial rise in e-cigarette (e-cig) use or vaping in the past decade, prompting growing concerns about their adverse health effects. Recently, e-cig manufacturers have been using synthetic cooling agents, like WS-23 and WS-3, to provide a cooling sensation without the menthol taste. Studies have shown that aerosols/vapes generated by e-cigs can contain significant levels of reactive oxygen species (ROS). However, studies investigating the role of synthetic coolants in modulating ROS levels generated by e-cigs are lacking. This study seeks to understand the potential of synthetic coolants, e-cigarette additives that have become increasingly prevalent in e-liquids sold in the United States (US), on acellular ROS production. Aerosols were generated from e-liquids with and without synthetic coolants through a single-puff aerosol generator; subsequently, acellular ROS was semi-quantified in H2O2 equivalents via fluorescence spectroscopy. Our data suggest that adding WS-3 to e-liquid base (PG:VG), regardless of nicotine content, has a minimal impact on modifying e-cigarette-generated acellular ROS levels. Additionally, our data also suggest that the addition of WS-23 to nicotine-containing e-liquid base significantly modifies e-cigarette-generated acellular ROS levels. Together, our data provide insight into whether adding synthetic coolants to e-liquids significantly impacts vaping-induced oxidative stress in the lungs.

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“…Despite being widely considered a safer alternative to conventional cigarettes, e-cigarettes were shown to exert biological effects with marked clinical implications and therefore pose a serious public health issue. So far, the majority of in vitro studies of e-cigarettes effects on transcriptome and proteome focused on epithelial cells [22,[46][47][48][49] and not many data are present on fibroblasts. Lung fibroblasts, among other functions, have an important role in maintaining structural integrity and extracellular matrix of the lung and changes in their phenotype are associated with pathological conditions such as fibrosis and cancer progression [50,51].…”

Section: Discussionmentioning

confidence: 99%

Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells

et al. 2022

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Background Although still considered a safer alternative to classical cigarettes, growing body of work points to harmful effects of electronic cigarettes (e-cigarettes) affecting a range of cellular processes. The biological effect of e-cigarettes needs to be investigated in more detail considering their widespread use. Methods In this study, we treated V79 lung fibroblasts with sub-cytotoxic concentration of e-cigarette liquids, with and without nicotine. Mutagenicity was evaluated by HPRT assay, genotoxicity by comet assay and the effect on cellular communication by metabolic cooperation assay. Additionally, comprehensive proteome analysis was performed via high resolution, parallel accumulation serial fragmentation-PASEF mass spectrometry. Results E-cigarette liquid concentration used in this study showed no mutagenic or genotoxic effect, however it negatively impacted metabolic cooperation between V79 cells. Both e-cigarette liquids induced significant depletion in total number of proteins and impairment of mitochondrial function in treated cells. The focal adhesion proteins were upregulated, which is in accordance with the results of metabolic cooperation assay. Increased presence of posttranslational modifications (PTMs), including carbonylation and direct oxidative modifications, was observed. Data are available via ProteomeXchange with identifier PXD032071. Conclusions Our study revealed impairment of metabolic cooperation as well as significant proteome and PTMs alterations in V79 cells treated with e-cigarette liquid warranting future studies on e-cigarettes health impact.

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Comparison of biological and transcriptomic effects of conventional cigarette and electronic cigarette smoke exposure at toxicological dose in BEAS-2B cells

Cited by 17 publications

References 36 publications

The role of synthetic coolants, WS-3 and WS-23, in modulating E-cigarette-induced reactive oxygen species (ROS) in lung epithelial cells

The role of synthetic coolants, WS-3 and WS-23, in modulating E-cigarette-induced reactive oxygen species (ROS) in lung epithelial cells

Synthetic Coolant WS-23 increases E-Cigarette Generated Aerosolized Acellular Reactive Oxygen Species (ROS) Levels

Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells

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