Comparison of biofilm-associated cell survival following in vitro exposure of meticillin-resistant Staphylococcus aureus biofilms to the antibiotics clindamycin, daptomycin, linezolid, tigecycline and vancomycin

Central venous catheter-related biofilm infections: An up-to-date focus on meticillin-resistant Staphylococcus aureus

“…Moreover, recently there have been conflicting results on the use of vancomycin monotherapy in the treatment of biofilmembedded MRSA [41][42][43]. Rose and Poppens demonstrated that vancomycin exhibited bactericidal activity towards planktonic MRSA cells at low levels (1 mg/mL), but the MIC increased four-to eight-fold in a biofilm susceptibility assay, suggesting its use in combination with rifampicin or tigecycline [44].…”

“…An in vitro study of 12 MRSA clinical isolates showed that daptomycin was the most effective of five antibiotics tested (compared with clindamycin, linezolid, tigecycline and vancomycin) at killing cells within biofilms; in fact, although total killing of biofilm cells was not achieved, daptomycin treatment eliminated 96% of biofilmassociated bacteria [43].…”

One-stage Revision With Catheter Infusion of Intraarticular Antibiotics Successfully Treats Infected THA

“…Vancomycin concentrations well over 4000 lg/mL are readily obtained in the knee immediately after infusion, and with a daily infusion regimen, this level can be achieved daily for 6 weeks or longer [46]. Moderate concentrations of antibiotics are necessary to prevent biofilm formation [11,12], but once biofilm and persister cells have formed, concentrations of orders of magnitude greater (as high as 4000 lg/ mL) are necessary to eradicate the bacteria within the biofilm [7,8,11,12,17,27,31,45,49,50]. Clearly, some risk of toxicity exists with direct intraarticular infusion of antibiotics, but this method may be the safest way to achieve high antibiotic levels over time [54][55][56] while allowing for the opportunity to decrease the dose, change antibiotics, or stop them altogether in the case of allergic reactions or renal toxicity.…”

Investigation of Biofilm-Associated Antibiotic Susceptibilities of Methicillin-Resistant Staphylococci Isolated from Catheter-Related Nosocomial Infections

“…Smith ve arkadaşları 14 inhibitör ve bakterisidal konsantrasyonlarının planktonik hücre sonuçları ile yakın olması, bu çalışmada farklı bir bulgu olarak karşımıza çıkmış ve tigesiklinin KİE'de iyi bir seçe-nek olabileceğini düşündürmüştür.…”