Comparison of Biocompatibility of Calcium Silicate-Based Sealers and Epoxy Resin-Based Sealer on Human Periodontal Ligament Stem Cells

Abstract

The aim of this study was to evaluate the biocompatibility of calcium silicate-based sealers (CeraSeal and EndoSeal TCS) and epoxy resin-based sealer (AH-Plus) in terms of cell viability, inflammatory response, expression of mesenchymal phenotype, osteogenic potential, cell attachment, and morphology, of human periodontal ligament stem cells (hPDLSCs). hPDLSCs were acquired from the premolars (n = 4) of four subjects, whose ages extended from 16 to 24 years of age. Flow cytometry analysis showed stemness of hPDLSCs was maintained in all materials. In cell viability test, AH-Plus showed the lowest cell viability, and CeraSeal showed significantly higher cell viability than others. In ELISA test, AH-Plus showed higher expression of IL-6 and IL-8 than calcium silicate-based sealers. In an osteogenic potential test, AH-Plus showed a lower expression level than other material; however, EndoSeal TCS showed a better expression level than others. All experiments were repeated at least three times per cell line. Scanning electronic microscopy studies showed low degree of cell proliferation on AH-Plus, and high degree of cell proliferation on calcium silicate-based sealers. In this study, calcium silicate-based sealers appear to be more biocompatible and less cytotoxic than epoxy-resin based sealers.

Biological interactions between calcium silicate‐based endodontic biomaterials and periodontal ligament stem cells: A systematic review of in vitro studies

“…Two additional studies which met the inclusion criteria were found during the manual search of the references of the included studies. All 20 of the resulting studies were considered as eligible for qualitative synthesis after full-text evaluation (Collado-González et al, 2017a,b;Rodríguez-Lozano et al, 2017;Wang et al, 2018;Luo et al, 2018;Collado-González et al, 2019;Rodríguez-Lozano et al, 2019a,b;Lee et al, 2019;López-García et al, 2019Abuarqoub et al, 2020;Oh et al, 2020;Olcay et al, 2020;Rodríguez-Lozano et al, 2020a,b;Zheng et al, 2020;Gaudin et al, 2020;Sanz et al, 2020c;Jing et al, 2020).…”

“…Clinically, since CSCs/ CSSs are applied directly after mixing (before setting), the use of freshly mixed (unset) material eluates can better predict the initial response of dental stem cells towards them. Nevertheless, the use of incubated (set) material eluates could be useful to predict the late/long-term response after cellular renewal (Oh et al, 2020). The physicochemical differences between both types of sample preparations, such as pH (Pedano et al, 2018), may lead to different results in biological assays.…”

Positive and negative properties of four endodontic sealant groups: a systematic review

“…В исследовании H. Oh еt al. (2020) материал на основе эпоксидных смол показал низкий остеогенный потенциал, а также препятствие заживлению костной ткани при выведении за верхушку [11].…”

Characterization, Antimicrobial Effects, and Cytocompatibility of a Root Canal Sealer Produced by Pozzolan Reaction between Calcium Hydroxide and Silica

“…Therefore, slight moisture in root canal dentin may be advantageous to sealing, but resin-based sealer is affected by it [ 3 ]. Furthermore, several studies showed that MTA-based sealers have higher biocompatibility compared to resin-based sealers [ 4 , 5 , 6 ]. However, it is generally known that MTA has limited antimicrobial effects against some microorganisms [ 7 ].…”