Comparison of binding behavior for molecularly imprinted polymers prepared by hierarchical imprinting or Pickering emulsion polymerization

Abstract

The aim of this study was the evaluation of the binding performances and selectivity of molecularly imprinted beads prepared toward several penicillins (i) by hierarchical bulk polymerization in the pores of template-grafted silica microbeads (hMIPs) and (ii) by Pickering emulsion polymerization in the presence of template-decorated silica nanobeads (pMIPs). 6-Aminopenicillanic acid was chosen as the common fragmental mimic template. Both approaches produced micron-sized polymeric beads with good recognition properties toward the target ligands whereas the selectivity pattern appeared quite different. The polymer prepared by the Pickering emulsion approach showed binding properties similar to imprinted beads prepared by hierarchical approach. Equilibrium binding constants changed their values from 0.1-0.2 × 10(6) (hMIPs) to 0.2-0.6 × 10(6) M(-1) (pMIPs), while the binding site densities changed from 3.7-4.8 (hMIPs) to 0.3-0.55 μmol/g (pMIPs). Compared to the hierarchical polymerization, Pickering emulsion polymerization represents a more practical approach when a template mimic needs to be used.

Convenient synthesis of uncovered imprinted microspheres byGanoderma lucidumspore-stabilized pickering emulsion polymerization and their enhanced recognition of spiramycin

“…So far, there had been many researches on the MIPs which were prepared by Pickering emulsion polymerization. 26,32,33 These MIPs were usually covered with a layer of solid particles which were used as stabilizers in emulsion. Here, the important function of solid particles was to stabilize the Pickering emulsion and facilitate the formation of spherical MIPs.…”

Preparation of l‐phenylalanine‐imprinted solid‐phase extraction sorbent by Pickering emulsion polymerization and the selective enrichment of l‐phenylalanine from human urine

“…Ye and coworkers have used modified SiO 2 as a template for preparing MIP microspheres with well‐controlled hierarchical structures for the selective separation of β‐blockers , protein , and steroid . On the other hand, Baggiani and coworkers have used template‐decorated SiO 2 as a stabilizer for preparing MIP for penicillin. All the above‐mentioned MIPs prepared by the Pickering emulsion polymerization exhibited high recognition toward templates.…”

Pickering emulsion-based molecular imprinting

“…为了解决以上问题, Shen和Ye [8,9] [11] 、传感器 [12] 以及功能材料制备 [13,14] 等领域. [17] . 为了解决该问题, 本课题组 [9] 直 接将模板分子溶于油相, 采用类似的Pickering乳液 聚合方法合成分子印迹微球.…”