Comparison of BBL Crystal® ANR ID Kit and API rapid ID 32 A for Identification of Anaerobic Bacteria

Aim: It was the aim of this study to evaluate the clinical and microbiological differences between severe and local odontogenic abscesses. Methods: Thirty patients were prospectively enrolled. Sixteen of 30 patients suffered from a severe life-threatening abscess of the head and neck, whereas 14/30 patients presented with a localized submucous abscess. Anaerobic bacteria were identified and susceptibility testing was performed using E test strips for penicillin, amoxicillin + clavulanic acid, imipenem + cilastatin, clindamycin and metronidazole. Results: The mean duration until removal of all drains was 14.1 and 3.5 days, respectively. Anaerobic bacteria were found in all episodes of local abscesses, whereas 19% of the severe episodes were culture negative, and in 13%, only aerobes were identified. A total of 60 anaerobes were isolated from 27 patients (2.2 isolates/positive sample). The dominating species were Prevotella sp. (n = 17), Peptostreptococcus sp. (n = 15) and Propionibacterium sp. (n = 5). Eighty-seven percent of the isolates were susceptible to penicillin. Ninety-seven percent of the anaerobes were susceptible to amoxicillin + clavulanic acid, imipenem + cilastatin, and clindamycin. Eighty-three percent were susceptible to metronidazol. There was a tendency for a higher rate of episodes with penicillin-resistant bacteria in the patients with severe abscesses (14 vs. 31%). No difference in susceptibility regarding amoxicillin + clavulanic acid and clindamycin (7%) was observed.

show abstract

“…and Peptostreptococcus spp. is consistent with retrospective data [10,13] . The pathogenetic role of Candida spp.…”

Section: Discussionsupporting

confidence: 92%

Severe versus Local Odontogenic Bacterial Infections: Comparison of Microbial Isolates

Al‐Nawas

Maeurer

2007

Eur Surg Res

View full text Add to dashboard Cite

show abstract

“…The Gram stain results however corroborated well with the API 50 CHL data which test was more detailed and rigorous, consisting of fermentation assays with longer growth incubation duration compared with the API rapid ID 32A, which depended on preformed enzymes by mostly previously lyophilised species. This misidentification has also been previously reported (Moll et al, 1996) and shows that the biochemical tests may not in particular accurately discern phenotypic variability within members of the different genus.…”

Section: Resultssupporting

confidence: 55%

Evaluation of Commercial Probiotic Products

Fredua‐Agyeman

Parab

Gaisford

2016

British Journal of Pharmacy

View full text Add to dashboard Cite

“…Previous comparisons using other automated and manual commercial biochemical card systems have been variable (2,4,8,10,12,14,18) but disappointing for the identification of clostridia (3,13,16). These microorganisms are notoriously nonreactive in standard biochemical tests, and it is often necessary to perform analysis of volatile and nonvolatile acids by gas-liquid chromatography or perform sequence analyses.…”

Section: Discussionmentioning

confidence: 99%

“…For the identification of other anaerobic genera, results have been variable. Although the systems appear to perform well at the genus level (2,8), correct identification at the species level may vary from 30 to 80%, depending on the taxa (4,10,12,14,18).…”

mentioning

confidence: 99%

Multicenter Evaluation of the Vitek 2 Anaerobe and Corynebacterium Identification Card

et al. 2008

View full text Add to dashboard Cite

The new anaerobe and Corynebacterium (ANC) identification card for Vitek 2 was compared with a 16S rRNA gene sequencing (16S) reference method for accuracy in the identification of corynebacteria and anaerobic species. Testing was performed on a Vitek 2 XL system with modified software at three clinical trial laboratories. Reproducibility was determined with nine ATCC quality control strains that were tested 20 times over a minimum of 10 days at all three sites. A challenge set of 50 well-characterized strains and 365 recent fresh and frozen clinical isolates were included in the study. The expected positive and negative biochemical well reactions were also evaluated for substrate reproducibility. All strains were tested with the ANC card, and clinical isolates were saved for 16S rRNA gene sequencing. All reproducibility tests yielded expected results within a 95% confidence interval, except for that with Corynebacterium striatum ATCC 6940, for which identification failed at one trial site. For the challenge isolates, there was 98% correct identification, 5% low discrimination, and 2% incorrect identification, and 0% were unidentified. For clinical strains, there was 95.1% correct identification, 4.9% low discrimination, and 4.6% incorrect identification, and 0.3% were unidentified. The 4.6% (17/365) of clinical isolates that were incorrectly identified consisted of 14 isolates that were correct at the genus level and three that were incorrect at the genus level. The new ANC card met all performance criteria within a 95% confidence interval compared to the identification performance by 16S rRNA gene sequencing.

show abstract

Comparison of BBL Crystal® ANR ID Kit and API rapid ID 32 A for Identification of Anaerobic Bacteria

Cited by 13 publications

References 37 publications

Severe versus Local Odontogenic Bacterial Infections: Comparison of Microbial Isolates

Severe versus Local Odontogenic Bacterial Infections: Comparison of Microbial Isolates

Evaluation of Commercial Probiotic Products

Multicenter Evaluation of the Vitek 2 Anaerobe and Corynebacterium Identification Card

Contact Info

Product

Resources

About