Comparison of artificial sebum with human and hamster sebum samples

Lu, Guang Wei; Valiveti, Satyanarayana; Spence, Julie; Zhuang, Christine; Robosky, Lora C.; Wade, Kimberly; Love, Ann; Hu, Lufei; Pole, David L.; Mollan, Matt

doi:10.1016/j.ijpharm.2008.09.025

Cited by 47 publications

(39 citation statements)

References 16 publications

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“…MICs in the presence or absence of 50% artificial sebum (40) were determined using the broth microdilution method in Wilkins-Chalgren broth (Becton, Dickinson & Co., Sparks, MD) as described in the Clinical and Laboratory Standards Institute (CLSI) methodology for anaerobic bacteria (7,55). Sixteen P. acnes isolates were tested once per isolate, and then the MIC 50 s and MIC 90 s were calculated accordingly.…”

Section: Methodsmentioning

confidence: 99%

“…Although aerotolerant, P. acnes typically grows in the anaerobic environment of the infrainfundibulum of the pilosebaceous unit, where it releases lipases and digests local accumulations of the skin and sebum (oily substance produced by sebaceous glands). Sebum is primarily composed of glycerides, triglycerides, wax esters, squalene, and free fatty acids (40,61,62).…”

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confidence: 99%

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In Vitro Antibacterial Activity of NB-003 against Propionibacterium acnes

Pannu

McCarthy

Martin

et al. 2011

Antimicrob Agents Chemother

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NB-003 and NB-003 gel formulations are oil-in-water nanoemulsions designed for use in bacterial infections. In vitro susceptibility of Propionibacterium acnes to NB-003 formulations and comparator drugs was evaluated. Both NB-003 formulations were bactericidal against all P. acnes isolates, including those that were erythromycin, clindamycin, and/or tetracycline resistant. In the absence of sebum, the MIC 90 s/minimum bactericidal concentrations (MBC 90 s) for NB-003, NB-003 gel, salicylic acid (SA), and benzoyl peroxide (BPO) were 0.5/2.0, 1.0/2.0, 1,000/2,000, and 50/200 g/ml, respectively. In the presence of 50% sebum, the MIC 90 s/MBC 90 s of NB003 and BPOs increased to 128/1,024 and 400/1,600 g/ml, respectively. The MIC 90 s/MBC 90 s of SA were not significantly impacted by the presence of sebum. A reduction in the MBC 90 s for NB-003 and BPO was observed when 2% SA or 0.5% BPO was integrated into the formulation, resulting in MIC 90 s/MBC 90 s of 128/256 g/ml for NB003 and 214/428 g/ml for BPO. The addition of EDTA enhanced the in vitro efficacy of 0.5% NB-003 in the presence or absence of 25% sebum. The addition of 5 mM EDTA to each well of the microtiter plate resulted in a >16-and >256-fold decrease in MIC 90 and MBC 90 , yielding a more potent MIC 90 /MBC 90 of <1/<1 g/ml. The kinetics of bactericidal activity of NB-003 against P. acnes were compared to those of a commercially available product of BPO. Electron micrographs of P. acnes treated with NB-003 showed complete disruption of bacteria. Assessment of spontaneous resistance of P. acnes revealed no stably resistant mutant strains.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

In Vitro Antibacterial Activity of NB-003 against Propionibacterium acnes

Pannu

McCarthy

Martin

et al. 2011

Antimicrob Agents Chemother

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“…In vitro diffusion in medium chain triglyceride (MCT) was evaluated by mixing the nanoparticles suspension (250µl) with MCT (500µl) and testing the fluorescence in the MCT at different time intervals. For testing the suitability of the different nanoparticles for the investigation of nanoparticles skin penetration and the stability of the markers upon contact with skin lipids and sebum, in vitro release in artificial sebum was performed using the same above procedure utilizing the composition of sebum described by (Lu et al, 2009). Particles were incubated with the sebum, samples in aliquots composed of 0.25µl nanoparticles with 0.5 g of sebum and the aliquots were mixed by vortex to ensure efficient contact between the NPs and sebum then kept shaking at 37°C.…”

Section: 23mentioning

confidence: 99%

Stability of fluorescent labels in PLGA polymeric nanoparticles: Quantum dots versus organic dyes

Abdel-Mottaleb

Béduneau

Pellequer

et al. 2015

International Journal of Pharmaceutics

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“…The amount and composition of the intracellular lipids change during the maturation process of the keratinocyte [60]. Sebaceous lipids containing large quantities of relatively low -melting-point fatty acids delivered to the skin surface is attenuated quite quickly as one proceeds (through the sequential tapestripping) into the membrane [61]. The relative ratio of these two components depends on the density of sebaceous glands and therefore on the body locations.…”

Section: Discussionmentioning

confidence: 99%

Potential of short-wave infrared spectroscopy for quantitative depth profiling of stratum corneum lipids and water in dermatology

Ezerskaia¹,

Uzunbajakava²,

Puppels³

et al. 2018

Biomed. Opt. Express

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Abstract:We demonstrate the feasibility of short wave infrared (SWIR) spectroscopy combined with tape stripping for depth profiling of lipids and water in the stratum corneum of human skin. The proposed spectroscopic technique relies on differential detection at three wavelengths of 1720, 1750, and 1770 nm, with varying ratio of the lipid-to-water absorption coefficient and an 'isosbestic point'. Comparison of the data acquired using SWIR spectroscopy with that obtained by a gold standard for non-invasive quantitative molecularspecific skin measurements, namely confocal Raman spectroscopy (CRS), revealed specificity of the proposed modality for water and lipid quantification. At the same time, we provide evidence showing aberrant sensitivity of Corneometer hydration read-outs to the presence of skin surface lipids, and a lack of sensitivity of the Sebumeter when attempting to measure the lipids of the cornified lipid envelope and intracellular lipid layers. We conclude that a spectroscopic SWIR-based spectroscopic method combined with tape stripping has the potential for depth profiling of the stratum corneum water and lipids, due to superior measurement sensitivity and specificity compared to the Corneometer and Sebumeter. References and links1. D. J. Tobin, "Introduction to skin aging," J. Tissue Viability 26(1), 37-46 (2017). 2. P. C. Arck, A. Slominski, T. C. Theoharides, E. M. Peters, and R. Paus, "Neuroimmunology of stress: skin takes center stage," J. Invest. Dermatol. 126(8), 1697-1704 (2006). 3. K. R. Feingold and P. M. Elias, "Role of lipids in the formation and maintenance of the cutaneous permeability barrier," Biochim. Biophys. Acta 1841(3), 280-294 (2014). 4. D. J. Tobin, "Biochemistry of human skin--our brain on the outside," Chem. Soc. Rev. 35(1), 52-67 (2006). 5. M. Akiyama, "Corneocyte lipid envelope (CLE), the key structure for skin barrier function and ichthyosis pathogenesis," J. Dermatol. Sci. 88(1), 3-9 (2017). 6. K. C. Madison, "Barrier function of the skin: "la raison d'être" of the epidermis," J. Invest. Dermatol. 121(2), 231-241 (2003). 7. D. J. Tobin, "Introduction to skin aging," J. Tissue Viability 26(1), 37-46 (2017 75-83 (2015). 13. R. J. Richters, N. E. Uzunbajakava, D. Falcone, J. C. Hendriks, E. J. Jaspers, P. C. van de Kerkhof, and P. E. van Erp, "Clinical, biophysical and immunohistochemical analysis of skin reactions to acute skin barrier disruptiona comparative trial between participants with sensitive skin and those with nonsensitive skin," Br. J. Dermatol. 174(5), 1126-1133 (2016). 14. M. Akiyama, "Corneocyte lipid envelope (CLE), the key structure for skin barrier function and ichthyosis pathogenesis," J. Dermatol. Sci. 88(1), 3-9 (2017). 15. M. Sullivan, N.B. Silverberg, "Current and emerging concepts in atopic dermatitis pathogenesis," Clin, Dermatol.35 (

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Comparison of artificial sebum with human and hamster sebum samples

Cited by 47 publications

References 16 publications

In Vitro Antibacterial Activity of NB-003 against Propionibacterium acnes

In Vitro Antibacterial Activity of NB-003 against Propionibacterium acnes

Stability of fluorescent labels in PLGA polymeric nanoparticles: Quantum dots versus organic dyes

Potential of short-wave infrared spectroscopy for quantitative depth profiling of stratum corneum lipids and water in dermatology

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