Comparison of arbitrarily primed polymerase chain reaction, ribotyping, and monoclonal antibody analysis for subtyping Legionella pneumophila serogroup 1

Gómez-Lus, P.; Fields, Barry S.; Benson, Robert F.; Martin, W. J.; O'Connor, S P; Black, Carolyn M.

doi:10.1128/jcm.31.7.1940-1942.1993

Cited by 75 publications

(40 citation statements)

References 13 publications

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“…RAPD assays afforded separation between S. typhimurium which did not exactly correspond to results of ribotyping, and a better discrimination corroborated by the field indications of isolation was obtained with ribotyping. This is in contrast with the findings of other workers which have demonstrated results of RAPD and ribotyping being either in complete concordance for the study of Proteus mirabilis isolates [ 131, or at a very high level of concordance for Legionella pneumophila strains [14]. In a study on 56 Escherichia coli isolates, Alos et al [lo]…”

Section: Rapd Techniquecontrasting

confidence: 76%

Comparison of random amplified polymorphic DNA analysis and enterobacterial repetitive intergenic consensus-PCR for epidemiological studies of Salmonella

Millemann¹

1996

FEMS Immunology and Medical Microbiology

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Discrimination of 70 Salmonella strains previously studied by ribotyping was realized by RAPD and ERIC-PCR analysis. RAPD results on the 56 S. typhimurium isolates did not closely match those of ribotyping. With ERIC-PCR, two fingerprints only were obtained. For the 14 S. enteritidis strains, a helpful discrimination was obtained with RAPD analysis, while ERIC-PCR resulted in a single fingerprint.

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Section: Rapd Techniquecontrasting

confidence: 76%

Comparison of random amplified polymorphic DNA analysis and enterobacterial repetitive intergenic consensus-PCR for epidemiological studies of Salmonella

Millemann¹

1996

FEMS Immunology and Medical Microbiology

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“…PFGE patterns were compared with 40 representative strains included in the Spanish PFGE type collection. (iii) Arbitrarily primed polymerase chain reaction (AP-PCR) was performed as previously described using the M13 forward primer (Gomez-Lus et al 1993). Gels were stained with ethidium bromide, visualized using UV transillumination and photographed prior to analysis.…”

Section: Genotyping Methodsmentioning

confidence: 99%

Persistence of chlorine-sensitiveLegionella pneumophilain hyperchlorinated installations

Garcı́a

Baladrón

Gil

et al. 2008

Journal of Applied Microbiology

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Aims: To study the persistence of Legionella over time in different disinfected facilities and analysing whether failures in bacterial eradication could be the result of a decrease in the susceptibility of the persistent strains to subsequent treatments. Methods and Results: A long‐term environmental surveillance was carried out in three different facilities associated with cases of Legionnaires’ disease (a hospital, a fishing boat and a hotel). Despite several hyperchlorination episodes, amplified fragment length polymorphism, pulsed field gel electrophoresis‐SfiI and arbitrarily primed polymerase chain reaction methods demonstrated that a specific clone of L. pneumophila serogroup 1 was able to survive for 17, 5 and 10 years in the hospital, fishing boat and the hotel, respectively. Isolates from different years from the same facility showed similar minimal inhibitory concentration and minimal bactericidal concentration values against eight different disinfectants. Conclusions: Hyperchlorination over long periods of time did not prevent the persistence of L. pneumophila. The lack of effectiveness did not appear to be the result of a decreased susceptibility of Legionella to chlorine. Hyperchlorination did not modify in vitro susceptibility of Legionella to other disinfectants to which the bacteria had not previously been exposed. Significance and Impact of the Study: Persistent Legionella in treated installations remain sensitive to disinfectants; hence, new strategies of treatment, different from hyperchlorination, should be developed to achieve bacterial eradication.

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“…Methods that use this approach comprise measurements of DNA relatedness over the entire genome, comparisons of restriction patterns, especially ribotyping, and comparative analyses of sequences of homologous genes (25). DNA-DNA relatedness and ribotyping are best suited for the identification of closely related species or strains within a single species (3,10,13,30,31). Presently, a direct comparison of rRNA sequences is probably the most powerful tool for the identification of many bacteria (25).…”

mentioning

confidence: 99%

Comparison of phenotypical and molecular methods for the identification of bacterial strains isolated from a deep subsurface environment

Boivin-Jahns¹,

Bianchi²,

Ruimy³

et al. 1995

Appl Environ Microbiol

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Seventy-four bacterial strains were freshly isolated from a mine gallery. Using these bacteria, we have investigated how a molecular identification based on the analysis of small subunit rDNA sequences would compare in terms of precision and reliability to a more classical comparison of phenotypical descriptions (100 morphological and physiological traits). Our data clearly showed that a phylogenetic analysis of small subunit rDNA sequences is more efficient than classical phenotypic methods for the identification of bacterial strains freshly isolated from a natural environment, because occurrences of misidentification are very much decreased by this method. The lack of rDNA sequences for many described species is probably the major cause of a few failures in molecular identification, as the completeness of the database of small subunit rDNA sequences holds much importance in the degree of uncertainty in such identifications.

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Comparison of arbitrarily primed polymerase chain reaction, ribotyping, and monoclonal antibody analysis for subtyping Legionella pneumophila serogroup 1

Cited by 75 publications

References 13 publications

Comparison of random amplified polymorphic DNA analysis and enterobacterial repetitive intergenic consensus-PCR for epidemiological studies of Salmonella

Comparison of random amplified polymorphic DNA analysis and enterobacterial repetitive intergenic consensus-PCR for epidemiological studies of Salmonella

Persistence of chlorine-sensitiveLegionella pneumophilain hyperchlorinated installations

Comparison of phenotypical and molecular methods for the identification of bacterial strains isolated from a deep subsurface environment

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