2003
DOI: 10.17221/3480-cjfs
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Comparison of antioxidative activity data for aqueous extracts of lemon balm (Melissa officinalis L.), oregano (Origanum vulgare L.), thyme (Thymus vulgaris L.), and agrimony (Agrimonia eupatoria L.) obtained by conventional methods and the DNA-based biosensor

Abstract: The antioxidative properties of aqueous plant extracts were evaluated using common methods such as the Rancimat and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) free radical method. Moreover, a voltammetric procedure based on the protective effect of antioxidants against the oxidative DNA damage was employed using a disposable DNA biosensor fabricated as a screen-printed electrode chemically modified by calf thymus double stranded (ds) DNA. The total polyphenols were also determined spectrophotometrically with the Fo… Show more

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Cited by 38 publications
(23 citation statements)
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“…Alkaloṏds were expressed as piperine equivalents (g PI/gFw) by comparing the absorbance values with a standard curve (0 Ð1 mg/ml of piperine). • Total phenolic compounds were determined using the Folin-Ciocalteau colorimetric method (Singleton et al 1999, Heilerová et al 2003. The extracts (500 l) were oxidized by 2.5 ml of the Folin-Ciocalteau reagent at 0.2 N and the reaction was neu- tralized with 2 ml of sodium carbonate at 75 g/L.…”
Section: Methodsmentioning
confidence: 99%
“…Alkaloṏds were expressed as piperine equivalents (g PI/gFw) by comparing the absorbance values with a standard curve (0 Ð1 mg/ml of piperine). • Total phenolic compounds were determined using the Folin-Ciocalteau colorimetric method (Singleton et al 1999, Heilerová et al 2003. The extracts (500 l) were oxidized by 2.5 ml of the Folin-Ciocalteau reagent at 0.2 N and the reaction was neu- tralized with 2 ml of sodium carbonate at 75 g/L.…”
Section: Methodsmentioning
confidence: 99%
“…The antioxidant properties were determined by the method of H e i l e r o v á et al [6]. The principle consists in the reaction of the coffee extract with a stable 2,2-diphenyl-1-picryl-hydrazyl (DPPH).…”
Section: Spectrophotometric Determination Of Antioxidant Activity By mentioning
confidence: 99%
“…Meat quality can be improved by incorporating natural herb and herb products (e.g., extracts, oils, leaves) based on the active ingredients with antioxidants in the animal diets. The most well-known herbs of the Labiatae family are Oregano (Cervato et al, 2000;Botsoglou et al, 2002) and lemon balm (Heilerová et al, 2003;Dastmalchi et al, 2008) are important sources of natural antioxidants. These herbs contains some chemical compounds such as flavonoids and phenolic compounds that have already been successfully used to increase the oxidative stability of meats because of shelf-life and meat quality when supplemented in chicken diets (Lahucky et al, 2010).…”
Section: Introductionmentioning
confidence: 99%