Comparison of Antimicrobial Susceptibility Testing of Campylobacter spp. by the Agar Dilution and the Agar Disk Diffusion Methods

Abstract

The correlation and the level of agreement between the standardized agar dilution and the agar disk diffusion methods for antimicrobial susceptibility testing of Campylobacter were investigated. A high-level agreement between the two methods was evident for aminoglycosides and fluoroquinolones, while a low-level agreement was observed for other antibiotics.

Prevalence and Antimicrobial Resistance of Campylobacter jejuni

“…In the current study, all isolates were susceptible or moderately susceptible to erythromycin. Unlike some surveillances (20,23,(28)(29)(30)(31)(32)(33)(34), we did not find macrolide-resistant strains.…”

Molecular identification and antimicrobial resistance profiles of Campylobacter strains of poultry origin in India with special emphasis on fluoroquinolone resistance

“…Susceptibility tests for campylobacter species are not standardized (there is no recommendation by the CLSI), so there are variabilities in the susceptibility reported (Vandenberg et al, 2006). However, we used disk diffusion method for the susceptibility testing of C. jejuni and C. coli strains used in this study followed by determination of MIC of the intermediately resistant and resistant strains by agar dilution method (Luangtongkum et al, 2007). Susceptibility testing of Campylobacter species is important to facilitate appropriate treatment where indicated and also for surveillance of emergence of drug resistance.…”

“…All Campylobacter strains were tested against ampicillin (10 µg), tetracycline (30 µg), azithromycin (15 µg), erythromycin (15 µg), chloramphenicol (30 µg), gentamicin (10 µg), nalidixic acid (30 µg), ciprofloxacin (5 µg), levofloxacin (5 µg), ofloxacin (5 µg), fosfomycin (50 µg) and sulphamethoxazole-trimethoprim (1.25 µg) by disk diffusion method as described by Luangtongkum et al (2007) with some modifications. Again, all intermediately resistant and resistant Campylobacter strains confirmed by disk diffusion method except sulphamethoxazole-trimethoprim resistant strains were subjected to the determination of minimum inhibitory concentration (MIC) by agar dilution method as described by Luangtongkum et al (2007) with some modifications.…”

“…Again, all intermediately resistant and resistant Campylobacter strains confirmed by disk diffusion method except sulphamethoxazole-trimethoprim resistant strains were subjected to the determination of minimum inhibitory concentration (MIC) by agar dilution method as described by Luangtongkum et al (2007) with some modifications. The antimicrobial resistance break points (Minimum inhibitory concentration: MIC) used were those established by national antimicrobial resistance monitoring system (NARMS) in accordance with Clinical and Laboratory Standards Institute (CLSI) guidelines: ampicillin, ≥32 µg ml -1 ; tetracycline, ≥16 µg ml -1 ; azithromycin, ≥8 µg ml -1 ; erythromycin, ≥8 µg ml -1 ; chloramphenicol, ≥32 µg ml -1 ; gentamicin, ≥16 µg ml -1 ; nalidixic acid, ≥32 µg ml -1 ; ciprofloxacin, ≥4 µg ml -1…”

<i>Campylobacter</i> Spp. Epidemiology and Antimicrobial Susceptibility in a Developing Country, Burkina Faso (West Africa)

“…However, the seasonality of campylobacteriosis is much debated (10). Several studies have reported good correlations between disc diffusion and agar dilution methods for the drug susceptibility testing of Campylobacter in developed and in developing countries (4, 15,35,36). In developing countries, various patterns of Campylobacter susceptibility to antibiotics were described.…”