Comparison of Antimicrobial Efficacy of Multiple Beef Hide Decontamination Strategies To Reduce Levels of Escherichia coli O157:H7 and Salmonella

Abstract

This study involved a comparison of the antimicrobial efficacy of several beef hide decontamination interventions to identify those that more effectively reduced levels of Escherichia coli O157:H7 and Salmonella. Whole beef hides were inoculated with E. coli O157:H7 and Salmonella and decontaminated with sprays of solutions of acetic acid (AA; 10%, 55 degrees C), lactic acid (LA; 10%, 55 degrees C), sodium hydroxide (SH; 3%, 23 degrees C), sodium metasilicate (SM; 4%, 23 degrees C), or sodium hydroxide (1.5%), followed by high-pressure washing with chlorinated (0.02%) water (SHC; both applied at 23 degrees C) or water (W; 23 degrees C) or by deluging with solutions of potassium cyanate (PC; 2.4%, 30 degrees C) or sodium sulfide (SS; 6.2%, 30 degrees C). All spraying treatments (AA, LA, SH, SM, and SHC) resulted in removal of visual organic material, whereas the dehairing treatments (PC and SS) successfully removed hair along with visual organic material. The PC, SS, and SHC treatments resulted in the greatest reductions of E. coli O157:H7 (P < 0.05), by 5.1, 4.8, and 5.0 log CFU/cm2, respectively. The SS and SHC treatments decreased Salmonella by 4.2 and 4.4 log CFU/cm2, respectively, compared with the water treatment, which reduced levels by 1.7 log CFU/cm2 (P < 0.05). The SH, AA, and LA treatments also lowered both E. coli O157:H7 and Salmonella by at least 2.0 log CFU/cm2. The treatments that were effective in this study deserve further consideration for commercial implementation as hide decontamination interventions.

Organic acids as antimicrobials to control Salmonella in meat and poultry products

“…Salmonella was reduced on skins by 3.4 and 2.8 logs when 10% lactic acid and 55°C were applied (Carlson, Ruby, Smith, Sofos, & Bellinger, 2008). Spraying of 2.0 and 4.0% lactic acids on beef trim surfaces resulted in reductions of E. coli O157:H7 and Salmonella of 2.0 and 1.5 logs, respectively (Harris, Miller, Loneragan, & Brashears, 2006).…”

Seasonal prevalence of potentially positive non-O157 Shiga toxin-producing Escherichia coli (STEC) bovine hides and carcasses in Costa Rica

“…Following the processing line, pre-eviscerated carcasses were tested for the potential presence of non-O157 STEC. Sampling at this point serves as an indicator of pathogen transfer from hides to carcasses mostly due to inadequate skinning practices, but may also reflect cross-contamination from equipment, workers, other carcasses, deposition of airborne bacteria generated by hide removal pullers, and the overall processing environment (Arthur et al, 2010;Carlson et al, 2008;Elder et al, 2000;O'Brien et al, 2005;Schmidt, Arthur, Bosilevac, Kalchayanand, & Wheeler, 2012). In the present investigation, the prevalence of potential positive non-O157 samples at this stage was significantly lower than that of the corresponding hide in all plants, regardless of the season and export status.…”

Synergistic Effects of Lactic Acid and Sodium Dodecyl Sulfate to Decontaminate Escherichia coli O157:H7 on Cattle Hide Sections

“…Hide sections were inoculated with 1 mL of adjusted culture at 10 8 CFU/mL using a pipette over the midline of a 5-cm · 10-cm area. The inoculum was then distributed uniformly by five vertical followed by five horizontal strokes (Carlson et al, 2008) using a sterile disposable L-shaped rod and left to dry for 5 min. Each inoculated area was uniformly treated by 12 hand sprays that delivered 10 g of dispensed volume.…”