Comparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine

Abstract

Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F 0 F 1 ATP synthase subunit α, succinyl-diaminopimelate desuccinylase, and cysteinyl-tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among animals should not be ignored and shows that the heterogeneity of antibody production against anti-staphylococcal antigens in animals may enable the identification of new immunotherapy targets.

Molecular Typing and Antimicrobial Susceptibility Profile of Staphylococcus aureus Isolates Recovered from Bovine Mastitis and Nasal Samples

“…We used 182 S. aureus isolates previously identified by biochemical tests [1,5]. All S. aureus isolates were further identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS; Bruker Daltonics, Bremen, Germany), as previously described by Nonnemann et al [6].…”

“…Moreover, it has great implications for milk production, the quality of milk and dairy products, antimicrobial usage, animal welfare, the environment, and the image of the dairy sector. Among other mastitis pathogens, Staphylococcus aureus is a major, prevalent mastitis pathogen, which represents a significant issue for bovine udder health, with unquestionable importance in human and veterinary medicine [1]. Although antibiotic treatment is widely used to fight bovine mastitis, S. aureus resistance to antimicrobials not only complicates antimicrobial treatment, but also represents a huge challenge for public health and food security, as cows are the major reservoir for the emergence of S. aureus human epidemic clones [2].…”

Immune System Evasion Mechanisms in Staphylococcus aureus: Current Understanding

“…These cells are called via an important chemoattractants such as C5a; leukotriene B4; and chemokines such as CXCL8 which also known as interleukin-8 (IL-8) in order to direct their actions to the site of infection 27 . This chemoattractants formed through activated host cells and some of them produced as an activated component of the complement system (C5a) at the time of identification of a preserved structure present on the bacterium 28 . All these chemoattractants trigger phagocytes by attaching to membrane bound receptors called G protein-coupled receptors (GPCRs).…”

“…3.b). As a result S. aureus has a wide range of strategies to evade and avoid phagocytic activity 28,29 .…”

>b<Molecular characterization of >i<Staphylococcus aureus>/i< and >i<Streptococcus uberis>/i< isolates of intramammary infections of dairy cows>/b<

“…Approaches to increase the cure of S. aureus IMI have been studied whether using the combination of antimicrobial treatment with vaccines (CUNHA et al, 2020;HAMBALI et al, 2018;MISRA et al, 2018;PEREIRA et al, 2011) or by extended therapy SKOULIKAS et al, 2018). However, it is not known whether there are genotypic differences in S. aureus isolates that cause mastitis during lactation before and after a treatment period.…”