Comparison of antibiotic resistance phenotypes in laboratory strains and clinical isolates of Staphylococcus aureus, Salmonella Typhimurium, and Klebsiella pneumoniae

Abstract

This study was designed to evaluate the antibiotic resistance phenotypes in wild-type (WT-SA), oxacillin-induced (OI-SA), clinically-acquired antibiotic-resistant (CA-SA), wild-type Typhimurium (WT-ST), ciprofloxacin-induced . Typhimurium (CI-ST), clinically-acquired antibiotic-resistant. Typhimurium (CA-ST), wild-type (WT-KP), ciprofloxacin-induced (CI-KP), and clinically-acquired antibiotic-resistant (CA-KP). The resistance of WT-SA, WT-ST, and WT-KP to ampicillin, ceftazidime, and cephalotin, penicillin was increased after induction by oxacillin OI-SA, ciprofloxacin CI-ST, and ciprofloxacin CI-KP, respectively. The highest β-lactamase activities were 12 and 36 μmol/min/ml, respectively, for CA-ST and CA-KP. The EtBr residues remained high in. Typhimurium (>80%) and (>90%) when treated with CCCP. The distinct FT-IR spectra were observed in protein region (1700-1500 cm) and carbohydrate region (1200-900 cm). This study would provide useful information for better understating of specific resistance mechanisms in association with β-lactamase and efflux pump activities.

Antibiotic resistance pattern of Klebsiella pneumoniae in obtained samples from Ziaee Hospital of Ardakan, Yazd, Iran during 2016 to 2017

“…In recent years, antibiotics have been used to treat diseasecausing bacteria. Antimicrobial resistance is a global threat to public health [1][2][3]. Enzymatic modification (b-lactamases), reduced permeability (selective blockage), increased membrane transport (efflux pumps), altered binding site (specific receptors) and metabolic bypass are the mechanisms of antibiotic resistance in bacteria [4][5][6].…”

First Insights into Clinical and Resistance Features of Infections by Klebsiella pneumoniae among Oncological Patients from a Referral Center in Amazon Region, Brazil

“…A study by Jo et al [ 29 ] demonstrated that in cancer patients, antimicrobial resistance in K. pneumoniae is linked to misuse and/or overuse of chemotherapeutic agents. Due to a decreased immunity in individuals undergoing antineoplastic chemotherapy, opportunistic pathogens such as K. pneumoniae can cause a wide range of infections, including pneumonia, urinary tract infection, bacteremia and meningitis [ 30 ].…”