Comparison of Antibacterial Effects of 810 and 980- nanometer Diode Lasers on Enterococcus Faecalis in the Root Canal System-An <i>in vitro</i> study

Abstract

Background and aim:Use of laser technology in endodontics has greatly increased in the recent years due to the introduction of new wavelengths and methods and optimal antimicrobial and smear layer removal properties of lasers. This in vitro study aimed to compare the antibacterial effects of diode lasers of 810 nm and 980 nm wavelength on Enterococcus faecalis (E. faecalis) biofilm in the root canal system. Materials and methods: Fifty single-canal human anterior teeth were cleaned, shaped, sterilized and randomly divided into four groups namely two experimental, one positive and one negative control group. The experimental and positive control groups were inoculated with E. faecalis and incubated for two weeks. The experimental group one (n=20) received 810 nm diode laser irradiation (1.5W) while the experimental group two (n=20) was subjected to 980 nm diode laser irradiation (1.5W). The E. faecalis colony forming units (CFUs) were counted in each root canal before and after laser irradiation. Results: Laser irradiation significantly decreased the bacterial colony count in both experimental groups. The reduction in microbial count was significantly greater in 810 nm laser group compared to 980 nm laser group. Conclusion: Irradiation of both 810 and 980 nm lasers significantly decreased the E. faecalis count in the root canal system; 810 nm laser was more effective in decreasing the intracanal microbial load.

Application of a Medical Diode Laser (810 nm) for Disinfecting Small Microbiologically Contaminated Spots on Degraded Collagenous Materials for Improved Biosafety in Objects of Exceptional Historical Value From the Auschwitz-Birkenau State Museum and Protection of Human Health

“…Previous studies on the antimicrobial effects of diode laser irradiation were mainly conducted in medicine and were focused on pathogenic bacteria such as Enterococcus faecalis and Escherichia coli. Nevertheless, higher values of average laser power than in this paper were most often applied (Beer et al, 2011;Asnaashari et al, 2016;Olivi et al, 2016;Dai et al, 2018), with similar ones applied less often (Tosun et al, 2012;Sadık et al, 2013).…”

Effect of a 980-nm diode laser on post-operative pain after endodontic treatment in teeth with apical periodontitis: a randomized clinical trial

“…The use of diode lasers, in addition to conventional endodontic therapy, has recently been proposed in RCT [16]. Various researchers have observed effective disinfection of the root canal by diode laser irradiation [17][18][19]. Due to the large water transmission capacities of diode lasers (810, 940 and 980-nm wavelengths), they can reach bacteria in deeper layers of dentinal tubules [20].…”

980-nm diode laser excision of a giant pregnancy epulis

“…Study has shown that the bactericidal effect of an 810-nm diode laser is better than that of a 980-nm laser, because of greater penetration into tissue. 7 As shown in this case, the 980-nm diode laser surgery might have some advantages over the conventional surgery of pregnancy epulis resection. Firstly, the 980-nm diode laser surgery causes little trauma and almost no bleeding.…”