Comparison of anti-pathogenic activities of the human and bovine milk N-glycome: Fucosylation is a key factor

Wang, Wenli; Wang, Wei; Du, Yamin; Wu, Hong; Yu, Xiaobo; Ye, Ke-Ping; Li, Chunbao; Jung, Yong‐Sam; Qian, Yingjuan; Voglmeir, Josef; Liu, Li

doi:10.1016/j.foodchem.2017.05.026

Cited by 31 publications

(52 citation statements)

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“…Both milk N ‐glycans and free oligosaccharides consist of pools of various sugars with different structures. The quantification of the sample was carried out as previously reported (Wang et al., 2017). Briefly, commercially available maltopentaose was used as an internal standard (20 µmoL/mL) to measure the amount of N ‐glycans, and as an external standard (0.8, 0.6, 0.4, 0.2, 0.1, 0.05, 0.005, 0.0005 M) to quantify MOs based on the determined UPLC peak area, using a previously described analysis method based on hydrophilic interaction liquid chromatography (HILIC)‐UPLC (Nexera, Shimadzu Corporation, Kyoto, Japan), which consists of an LC‐30AT pump system and an RF‐20Axs fluorescence detector set at the excitation/emission wavelengths of 330/420 nm, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Solvent A was aqueous 50 mM ammonium formate buffer, pH 4.5 and solvent B was acetonitrile. The separation of the analytes was carried out using a previously developed linear gradient of solvent A and B (Supporting information Table S1)(Wang et al., 2017). The quantification of N ‐glycans was calculated based on the following formula (the peak area means the area of all the UPLC peaks):

N - normalglycan normalconcentration = \frac{0 . 02 normalmM \times normalpeak normalarea}{maltopentaose peak area}

…”

Section: Methodsmentioning

confidence: 99%

“…Similar to free MOs, N ‐glycans derived from bovine milk are highly sialylated and slightly fucosylated, while human and goat milk N ‐glycans (GMNs) are highly fucosylated (Le Parc et al., 2014; Nwosu et al., 2012). Milk N ‐glycans have not been studied as extensively as MOs and were only recently described to be antipathogenic (Barboza et al., 2012; Rossi et al., 2002; Wang et al., 2017). N ‐glycosylation was also found to confer higher antipathogenic activity to glycoproteins when using Listeria monocytogenes as a model pathogen (Zheng et al., 2019).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of the antipathogenic effect toward Staphylococcus aureus of N‐linked and free oligosaccharides derived from human, bovine, and goat milk

Yue

Han

Yin

et al. 2020

Journal of Food Science

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N‐linked oligosaccharides (N‐glycans) derived from milk were recently found to be antipathogenic. This study compares the antimicrobial activity of N‐linked glycans and free oligosaccharides from human, bovine, and goat milk against Staphylococcus aureus. Milk N‐glycans showed a bactericidal/bacteriostatic effect on the pathogen when compared to free milk oligosaccharides, evidenced by the clear zone from the halo assay, with the order of human milk >goat milk >bovine milk. None of the free milk oligosaccharide samples were bactericidal/bacteriostatic, despite its positive results in growth curve and minimum inhibitory concentration (MIC) assays which are believed to be related to hyperosmosis. Both N‐glycans and free milk oligosaccharides can reduce the adhesion of Staphylococcus aureus to Caco‐2 cells, however, N‐glycans worked significantly more effective than free milk oligosaccharides. Structural analysis of all free oligosaccharide and N‐glycan samples showed the obvious interspecies differences, and the structure/function relationship of the respected N‐glycans is of interest for future study. The significant bactericidal/bacteriostatic activity possessed by human, bovine, and goat milk N‐linked glycans holds great potential as a novel substitute for antibiotics.

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Section: Methodsmentioning

confidence: 99%

N - normalglycan normalconcentration = \frac{0 . 02 normalmM \times normalpeak normalarea}{maltopentaose peak area}

…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of the antipathogenic effect toward Staphylococcus aureus of N‐linked and free oligosaccharides derived from human, bovine, and goat milk

Yue

Han

Yin

et al. 2020

Journal of Food Science

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“…Fucosylation profiles of HMOs and HMGPs have been more broadly studied because of their abundance in human milk, whereas sialylated profiling is challenging since it requires the removal of dominant fucosylated structures and lactose (Yan et al, 2018). Nonetheless, differences in molecular structure among species, or among human individuals, are now better documented (Chaturvedi et al, 2001;Sischo, Short, Geissler, Bunyatratchata, & Barile, 2017;W. L. Wang et al, 2017) and might lead to more personalized advice on type and variation of HMOs and HMGPs in infant formulas.…”

Section: Host Genetic Factorsmentioning

confidence: 99%

Relationship Between Oligosaccharides and Glycoconjugates Content in Human Milk and the Development of the Gut Barrier

Figueroa-Lozano

Vos

2018

Comp Rev Food Sci Food Safe

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The intestinal immune barrier is considered to be the gatekeeper of the human body and rapidly develops directly after birth. Many pre‐ and postnatal factors influence the development of the gut‐barrier, which is composed of the microbiota, the mucus, the epithelial layer and the mucosal immune system. Even minor disturbances during barrier development can have consequences for health far into adulthood. Here we critically discuss the current knowledge on which pre‐ and postnatal factors influence development, maturation, and maintenance of the gut immune barrier. Human milk has a unique composition and is the gold standard for adequate development of the intestinal immune barrier. Not only the influence of human milk oligosaccharides (HMOs) but also that of glycoproteins (HMGPs) is reviewed. We discuss the influence of maternal genetic factors, such as the secretor and Lewis phenotypes on breast milk fucosylation and sialylation of HMOs and HMGPs. This diversity in HMOs and HMPGs influences microbiota composition and also the development of the immune barrier. Cow milk‐derived infant formula is often being used as an alternative for human breast milk. The consequences of this for proper development of the intestinal immune barrier and, in particular, the differences in the type of oligosaccharides and glycosylation patterns (sialic and fucose composition) between cow and human milk are critically discussed. Current and prospective strategies to promote proper gut‐immune maturation are proposed. These might include more personalized infant formulas when breast milk is not an option.

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“…Complex sugars are abundant in the milk of many species, though not all, and are active as free or protein-linked oligosaccharides [12,[63][64][65][66]. In humans, these complex sugars vary dramatically in quantity and types between mothers [12,67].…”

Section: Oligosaccharidesmentioning

confidence: 99%

Rapid changes in Atlantic grey seal milk from birth to desertion

Bawazeer

et al. 2017

Preprint

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True seals have the shortest lactation periods of any group of placental mammal. Most are capital breeders that undergo short, intense lactations, during which they fast while transferring substantial proportions of their body reserves to their pups, which they then abruptly desert. Milk was collected from Atlantic grey seals (Halichoerus grypus) periodically from birth until near desertion. Milk protein profiles matured within 24 hours or less, indicating the most rapid transition from colostrum to mature phase lactation yet observed. There was an unexpected persistence of immunoglobulin G almost until weaning, potentially indicating prolonged trans-intestinal transfer of IgG. Among components of innate immune protection were found fucosyllactose and siallylactose that are thought to impede colonisation by pathogens and encourage an appropriate gut microbiome. These oligosaccharides decreased from early lactation to almost undetectable levels by weaning. Taurine levels were initially high, then fell, possibly indicative of taurine dependency in seals, and progressive depletion of maternal reserves. Metabolites that could signal changes in the mother's metabolism of fats, such as nicotinamide and derivatives, rose from virtual absence, and acetylcarnitines fell. It is therefore possible that indicators of maternal metabolic strain exist that signal the imminence of desertion.

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Comparison of anti-pathogenic activities of the human and bovine milk N-glycome: Fucosylation is a key factor

Cited by 31 publications

References 31 publications

Comparison of the antipathogenic effect toward Staphylococcus aureus of N‐linked and free oligosaccharides derived from human, bovine, and goat milk

Comparison of the antipathogenic effect toward Staphylococcus aureus of N‐linked and free oligosaccharides derived from human, bovine, and goat milk

Relationship Between Oligosaccharides and Glycoconjugates Content in Human Milk and the Development of the Gut Barrier

Rapid changes in Atlantic grey seal milk from birth to desertion

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