Comparison of Animal Sera for Suitability in Coagulase Testing 1

Orth, D. S.; Chugg, L. R.; Anderson, A. W.

doi:10.1128/aem.21.3.420-425.1971

Cited by 23 publications

(16 citation statements)

References 9 publications

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“…The present 91.3% sensitivity was found in agreement and lower than 94-100% previous findings reported by other investigators (Tager et al, 1948, Orth et al, 1971Oranusi and Umoh, 2006)…”

Section: Dnase and Coagulase Testsupporting

confidence: 90%

Isolation, DNase-cross-Coagulase test and antimicrobial resistance test on <i>Staphylococcus</i> along beef abattoir line in Addis Ababa Ethiopia

Hiko¹

2019

Ethiop. Vet. J.

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Staphylococci are responsible for foodborne infection and intoxication with the spread of antibiotic resistance. The aims of the study were to investigate beef abattoir line contamination with Staphylococcal, to evaluate DNase test for alternative of the tube coagulase test, and to assess isolates drug resistance in Ethiopia. A total of 169 samples from slaughter environment, raw beef at inspection and at public supply along Addis Ababa Abattoir Enterprise line were examined for Staphylococci. The isolates were tested against DNase, tube plasma coagulase, and eight medicinal drugs. A total proportion of 35.5% (60/169) isolates with 13.6% S. aureus and 21.9% coagulase negative Staphylococcus (CNS) were observed. All sampling locations were found positive for Staphylococcus environmental samples ranged from 18.2% -46.2% with no difference (p > 0.05) among locations. In raw beef, it was 23.5% at abattoir and 52.9% at butchers. Three (1.9%), 13 (7.7%) and 23 (13.6%) of locations were positive for S. aureus only, CNS only and both as a mixed, respectively. Of all 60 Staphylococci isolates, the DNase test and coagulase tests were in agreement for 56 isolates (21 for positive, 35 for negative) showing DNase test was strong agreement with the gold standard test (coagulase tests), kappa=0.86)). S. aureus was 38.3% but CNS was 61.7%. Resistant isolates were observed for trimethoprim (35.0%), polymyxin-B (33.7%), oxytetracycline (31.7%), trimethoprim-sulfamethoxazole (20.0%), chloramphenicol (8.3%), oxacillin (6.7%), and gentamycin (5.0%) but not for tetracycline. Thirty-one (51.7%) isolates were resistant for at least one drug with multiple drugs resistance (MDR) of three to six in 17 isolates. Contamination of all sampling locations with Staphylococcus including with resistant isolate to medically used drugs warrants the application of good hygienic practices along the abattoir line. Due to availability and cost effectiveness, DNase can be used as alternative to the gold standard, coagulase test, for diagnosis of Staphylococcus.

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“…The present 91.3% sensitivity was found in agreement and lower than 94-100% previous findings reported by other investigators (Tager et al, 1948, Orth et al, 1971Oranusi and Umoh, 2006)…”

Section: Dnase and Coagulase Testsupporting

confidence: 90%

Isolation, DNase-cross-Coagulase test and antimicrobial resistance test on <i>Staphylococcus</i> along beef abattoir line in Addis Ababa Ethiopia

Hiko¹

2019

Ethiop. Vet. J.

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“…We investigated the sensitivities of pig plasma, rabbit plasma, and pig-rabbit plasma systems in detecting the coagulase reaction on solid agar plates. Rabbit plasma is the accepted plasma for coagulase testing, whereas pig plasma has been shown by some investigators to increase the sensitivity of the test (9,14). Preliminary experiments revealed that pig plasma alone was unsatisfactory because of inadequate and undependable reactions.…”

Section: Resultsmentioning

confidence: 99%

Tandem Coagulase/Thermonuclease Agar Method for the Detection of Staphylococcus aureus

Boothby

Genigeorgis

Fanelli

1979

Appl Environ Microbiol

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In optimizing previously reported coagulase agar media to obtain a rapid, reliable, and inexpensive coagulase test agar, variations in plasmas, pH, buffer system, fibrinogen, and fibrinolytic inhibitor were investigated. The agar with the following composition was determined best for the demonstration of coagulase production by Staphylococcus aureus: 25 ml of 15% bovine fibrinogen (fraction I, type I, citrated, Sigma Chemical Co.), 25 ml of rehydrated rabbit plasma (coagulase plasma ethylenediaminetetraacetic acid, Difco), 10.0 mg of soybean trypsin inhibitor (Schwarz/Mann), and 450 ml of brain heart infusion agar (Difco). In additional studies involving 7 different temperatures and 11 heating times, the thermal destruction of microbial nucleases on plate count agar and coagulase test agar was investigated. Heating the plates for 2.5 h at 65°C destroyed all heatlabile nucleases, but not thermonucleases of S. aureus. A tandem agar plate method for the identification of S. aureus was developed. Coagulase and thermonuclease activity of 50 colonies can be detected on a single agar plate. Suspect S. aureus colonies isolated on various selective media are transferred to coagulase test agar, the plates are incubated at 37°C for 18 h, and the coagulase reaction is recorded. The plates are then heated at 65°C for 2.5 h, overlaid with toluidine blue-metachromatic diffusion agar, and reincubated at 37°C for 3 h, and the thermonuclease reaction is recorded. Studies based on 88 enterotoxigenic S. aureus strains and 133 and 48 suspect S. aureus strains isolated from fresh salami mixtures on mannitol salt and tellurite-polymyxin-egg yolk agars, respectively, demonstrated 100% agreement between the tandem agar plate method and standard coagulase and thermonuclease tests. Overall, the tandem agar plate method is a rapid and convenient approach contributing to the identification of S. aureus from foods.

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“…Although Gross (3) reported on September 1, 2020 by guest http://aem.asm.org/ Downloaded from the use of swine plasma many years ago, its acceptability for coagulase testing has not received much attention. According to Orth et al (7), plasmin activity is greater in both rabbit and human plasmas than in swine plasma. As a result, staphylokinase and staphylococcal Muller factor activate the plasminogen-plasmin system, and the plasmin thus formed causes fibrinolysis and false negative reactions when rabbit plasma is used for coagulase testing.…”

Section: Resultsmentioning

confidence: 99%

Pour-Plate Method for the Detection of Coagulase Production by Staphylococcus aureus

Parisi¹,

Baldwin²,

Sottile³

1973

Applied Microbiology

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A pour-plate method is described for the detection of coagulase production by Staphylococcus aureus. Either Brain Heart Infusion agar or yeast extract-Trypticase soy agar containing swine plasma was the best medium for the detection of coagulase by this method. The advantages of this method and its potential utilization in the clinical laboratory, in food microbiology, and in specialized studies with S. aureus are discussed.

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Comparison of Animal Sera for Suitability in Coagulase Testing 1

Cited by 23 publications

References 9 publications

Isolation, DNase-cross-Coagulase test and antimicrobial resistance test on <i>Staphylococcus</i> along beef abattoir line in Addis Ababa Ethiopia

Isolation, DNase-cross-Coagulase test and antimicrobial resistance test on <i>Staphylococcus</i> along beef abattoir line in Addis Ababa Ethiopia

Tandem Coagulase/Thermonuclease Agar Method for the Detection of Staphylococcus aureus

Pour-Plate Method for the Detection of Coagulase Production by Staphylococcus aureus

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