Comparison of an inactive submarine spring with an active nearshore anchialine spring in Florida

Garman, K. Michael; Rubelmann, Haydn; Karlen, David J.; Wu, Tiehang; Garey, James R.

doi:10.1007/s10750-011-0740-2

Cited by 25 publications

(19 citation statements)

References 46 publications

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“…The presence of sulfur reducers likely accounts for the 30 mg/L of sulfide detected within this layer ( Table 1). It has been reported in a similar system that Archaea contribute more to sulfur reduction than Bacteria [10], but this is not corroborated by the Archaeal functional analysis ( Table 3), suggesting that a significant population of Archaeal sulfur reducers was present, but not detected by our sequence analysis.…”

Section: Hospital Hole: the Ecosystemcontrasting

confidence: 74%

“…Two genera, Vulcanibacillus and Thiobacillus, within Hospital Hole (Supplemental Table S1) were found in the Edwards Aquifer [7]. Sulfur reducers in the anoxic layer of Hospital Hole (Table 2), in Jewfish Sink [10] and the saltwater portion of Edwards Aquifer [7] were all within the order Desulfobacterales. Jewfish Sink and Hospital Hole had similar microbes.…”

Section: Hospital Hole: the Ecosystemmentioning

confidence: 99%

“…Jewfish Sink and Hospital Hole had similar microbes. Arcobacter, Sulfurimonas, Desulfosarcina, Desulfofaba, Clostridium, Spirochaeata, Dehalococcoides, Cytophaga, Pelobacter and Desulfobacterium were present within both Hospital Hole (Table 2) and Jewfish Sink [10], which are 14 km apart. Sphingomonas was present in the Wakulla spring system in North Florida [8] and in Hospital Hole (Supplemental Table S1).…”

Section: Hospital Hole: the Ecosystemmentioning

confidence: 99%

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Microbial Function and Hydrochemistry within a Stratified Anchialine Sinkhole: A Window into Coastal Aquifer Interactions

Davis

Garey

2018

Water

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Anchialine sinkholes provide insight into coastal aquifer systems and coastal mixing processes. Aquifer microbial community function is usually inferred from hydrochemical information, but there are few direct studies of microbial communities in the Floridan Aquifer. Hospital Hole is a 43 m-deep stratified sinkhole under the Weeki Wachee River, FL, with three distinct brackish layers: a hypoxic layer, a chemocline and a sulfidic anoxic layer. Illumina sequencing and bioinformatic tools were used to reconstruct metabolic functions and interactions of microbial communities in each layer. Each layer appears to originate from different parts of the coastal mixing zone and has a distinct microbial community with unique functions, which are influenced by the respective hydrochemistry. Sulfide oxidation and nitrate reduction are the most abundant functions. Syntrophy between methane oxidizers, methanogens and sulfate reducers is present. Similarities between the hydrochemistry and potential connectivity of Hospital Hole and the Floridan Aquifer coastal mixing zone suggest that microbial communities of Hospital Hole could be a surrogate for the coastal mixing zone of the aquifer in the absence of direct studies. Understanding how groundwater microbial communities react to saltwater intrusion and nutrient flux will be useful in predicting how coastal aquifer regions might react to anthropogenic change.

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Section: Hospital Hole: the Ecosystemcontrasting

confidence: 74%

Section: Hospital Hole: the Ecosystemmentioning

confidence: 99%

Section: Hospital Hole: the Ecosystemmentioning

confidence: 99%

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Microbial Function and Hydrochemistry within a Stratified Anchialine Sinkhole: A Window into Coastal Aquifer Interactions

Davis

Garey

2018

Water

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“…All environmental sequences in GenBank, which were affiliated with the Ancoroidea (Fig. 8), were obtained from anoxic marine sediments, including cold methane seeps and shallow water hydrothermal zones (Edgcomb et al, 2002;Stoeck, Taylor & Epstein, 2003;Stoeck et al, 2007;Takishita et al, 2007;Santos et al, 2010;Boere et al, 2011;Garman et al, 2011;Orsi et al, 2012). The geographical distribution of samples containing the ancoroid sequences is wide: arctic, temperate, and tropical zones of the Atlantic and Indo-Pacific regions:…”

Section: Molecular and Morphological Diversity In Ancoroideamentioning

confidence: 99%

Peer Review #1 of "A new view on the morphology and phylogeny of eugregarines suggested by the evidence from the gregarine Ancora sagittata (Leuckart, 1860) Labbé, 1899 (Apicomplexa: Eugregarinida) (v0.1)"

2017

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Background. Gregarines are a group of early branching Apicomplexa parasitizing invertebrate animals. Despite their wide distribution and relevance to the understanding the phylogenesis of apicomplexans, gregarines remain understudied: light microscopy data are insufficient for classification, and electron microscopy and molecular data are fragmentary and overlap only partially. Methods. Scanning and transmission electron microscopy, PCR, DNA cloning and sequencing (Sanger and NGS), molecular phylogenetic analyses using ribosomal RNA genes (18S (SSU), 5.8S, and 28S (LSU) ribosomal DNAs (rDNAs)). Results & Discussion. We present the results of an ultrastructural and molecular phylogenetic study on the marine gregarine Ancora sagittata from the polychaete Capitella capitata followed by evolutionary and taxonomic synthesis of the morphological and molecular phylogenetic evidence on eugregarines. The ultrastructure of A. sagittata generally corresponds to that of other eugregarines, but reveals some differences in epicytic folds (crests) and attachment apparatus to gregarines in the family Lecudinidae, where A. sagittata has been classified. Molecular phylogenetic trees based on SSU (18S) rDNA reveal several robust clades (superfamilies) of eugregarines, including Ancoroidea superfam. nov., which comprises two families (Ancoridae fam. nov. and Polyplicariidae) and branches separately from the Lecudinidae; thus, all representatives of Ancoroidea are here officially removed from the Lecudinidae. Analysis of sequence data also points to possible cryptic species within A. sagittata and the inclusion of numerous environmental sequences from anoxic habitats within the Ancoroidea. LSU (28S) rDNA phylogenies, unlike the analysis of SSU rDNA alone, recover a well-supported monophyly of the gregarines involved (eugregarines), although this conclusion is currently limited by sparse taxon sampling and the presence of fast-evolving sequences in some species. Comparative morphological analyses of gregarine teguments and attachment organelles lead us to revise their terminology. The terms "longitudinal folds" and "mucron" are restricted to archigregarines, whereas the terms "epicystic crests" and "epimerite" are proposed to describe the candidate synapomorphies of eugregarines, which,

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“…Marine blue holes differ from anchialine blue holes, such as those found in the Bahamas and the Yucatán peninsula, as they do not have freshwater layers and are not exposed to the atmosphere. Anchialine blue holes can be highly stratified, with anoxic and sulfidic bottom waters (2)(3)(4) and microbial communities distinct from other marine and freshwater systems (5)(6)(7)(8). However, data on true marine blue holes are limited.…”

Section: Introductionmentioning

confidence: 99%

Gulf of Mexico blue hole harbors high levels of novel microbial lineages

Patin

Dietrich

Stancil

et al. 2020

Preprint

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Exploration of oxygen-depleted marine environments has consistently revealed novel microbial taxa and metabolic capabilities that expand our understanding of microbial evolution and ecology. Marine blue holes are shallow karst formations characterized by low oxygen and high organic matter content. They are logistically challenging to sample, and thus our understanding of their biogeochemistry and microbial ecology is limited. We present a metagenomic characterization of Amberjack Hole on the Florida continental shelf (Gulf of Mexico). Dissolved oxygen became depleted at the hole rim (32 m water depth), remained low but detectable in an intermediate hypoxic zone (40-75 m), and then increased to a secondary peak before falling below detection in the bottom layer (80-110 m), concomitant with increases in nutrients, dissolved iron, and a series of sequentially more reduced sulfur species. Microbial communities in the bottom layer contained heretofore undocumented levels of the recently discovered phylum Woesearchaeota (up to 58% of the community), along with lineages in the bacterial Candidate Phyla Radiation (CPR). Thirty-one high-quality metagenome-assembled genomes (MAGs) showed extensive biochemical capabilities for sulfur and nitrogen cycling, as well as for resisting and respiring arsenic. One uncharacterized gene associated with a CPR lineage differentiated hypoxic from anoxic zone communities. Overall, microbial communities and geochemical profiles were stable across two sampling dates in the spring and fall of 2019. The blue hole habitat is a natural marine laboratory that provides opportunities for sampling taxa with under-characterized but potentially important roles in redox-stratified microbial processes.

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Comparison of an inactive submarine spring with an active nearshore anchialine spring in Florida

Cited by 25 publications

References 46 publications

Microbial Function and Hydrochemistry within a Stratified Anchialine Sinkhole: A Window into Coastal Aquifer Interactions

Microbial Function and Hydrochemistry within a Stratified Anchialine Sinkhole: A Window into Coastal Aquifer Interactions

Peer Review #1 of "A new view on the morphology and phylogeny of eugregarines suggested by the evidence from the gregarine Ancora sagittata (Leuckart, 1860) Labbé, 1899 (Apicomplexa: Eugregarinida) (v0.1)"

Gulf of Mexico blue hole harbors high levels of novel microbial lineages

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