Comparison of an Immature Platelet Fraction and Reticulated Platelet in Liver Cirrhosis

Kim, Hye Ryun; Park, Bo Rae G.; Lee, Mi Kyung; Park, Ae Ja; Ahn, Jeong Yeal

doi:10.3343/kjlm.2007.27.1.7

Cited by 12 publications

(15 citation statements)

References 14 publications

(1 reference statement)

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“…The relatively lower cut-off value obtained in this study (2.6%) might be explained by the lower reference mean value (2.1%) estimated in the present study than in the previous studies (3.3% and 3.4% by Abe et al [1] and Cho et al [29], respectively). Our result is also supported by the report of Kim et al [28] in which their low mean reference value (1.7%) corresponded with a lower cut-off value (2.9%) similar to this study (2.6%).…”

Section: Discussionsupporting

confidence: 92%

“…[1], Kim et al [28], and Cho et al [29] reported cut-off values of 7.7%, 2.9%, and 6.1%, respectively. The relatively lower cut-off value obtained in this study (2.6%) might be explained by the lower reference mean value (2.1%) estimated in the present study than in the previous studies (3.3% and 3.4% by Abe et al [1] and Cho et al [29], respectively).…”

Section: Discussionmentioning

confidence: 99%

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Immature Platelet Fraction: Establishment of a Reference Interval and Diagnostic Measure for Thrombocytopenia

et al. 2010

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451Background: Immature platelet fraction (IPF, %) is a measure of reticulated platelets (RPs), which represents the state of thrombopoiesis. The IPF is obtained from an automated hematology analyzer as one of the platelet parameters. This study was performed to establish reference intervals of IPF and its cut-off values for the differential diagnosis of thrombocytopenia.Methods: Blood samples from 2,039 healthy individuals (1,161 males, 878 females) were obtained to establish reference intervals. The patient group included patients with idiopathic thrombocytopenic purpura (ITP) (N=150) and aplastic anemia (AA) (N=51) with platelet counts of less than 100×10 9 /L. We evaluated the reliability of the IPF measurements, the reference intervals, and cut-off value for the diagnosis of ITP.Results: The reference intervals of IPF were 0.5-3.2% in males and 0.4-3.0% in females (95% confidence interval). The median IPF% of ITP and AA were 7.7% (range, 1.0-33.8%) and 3.5% (range, 0.6-12.9%), respectively. Statistical analysis revealed a significant difference between the IPF% of ITP and AA (P<0.0001). The cut-off value of IPF for differentiating ITP from AA was 7.3% with a sensitivity and specificity of 54.0% and 92.2%, respectively.Conclusions: A rapid and inexpensive automated measurement of IPF can be integrated as a standard parameter to evaluate the thrombopoietic state of the bone marrow. This study determined the reference intervals of IPF from a large population of healthy individuals, including children. Further studies are needed to establish the clinical utility of IPF. (Korean J Lab Med 2010;30:451-9)

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Immature Platelet Fraction: Establishment of a Reference Interval and Diagnostic Measure for Thrombocytopenia

et al. 2010

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“…This parameter has the advantage that it can be obtained as part of a complete blood count (CBC) with little added cost. Although several studies have reported correlation between flow cytometric and automated methods-in animals and patient populations [15][16][17], no agreement between the two methods has been established yet. If clinical decisions are ultimately to be influenced by IPF measurements, particularly as new antiplatelet medications become available, it is essential to test the predictive ability of the new automated method to stratify immature platelet populations according to their response to antiplatelet therapy.…”

Section: Introductionmentioning

confidence: 95%

“…The measurement of the reticulated platelet fraction has required staining with thiazole orange and measurement of stained platelets using flow cytometric techniques [8][9][10][11][12][13][14][15][16][17]. However, flow cytometric techniques are costly, and time consuming.…”

Section: Introductionmentioning

confidence: 99%

Immature platelet fraction (IPF) determined with an automated method predicts clopidogrel hyporesponsiveness

Ibrahim

Nadipalli

DeLao

et al. 2011

J Thromb Thrombolysis

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The mechanisms for the variability in antiplatelet effects of clopidogrel are not elucidated entirely. Immature (reticulated) platelets may modulate the antiplatelet effects of clopidogrel but must be measured using flow cytometry. Whether new automated detection techniques yield similar results is not known. The objectives of the study to evaluate the role of immature platelets assessed by an automated method in response to the antiplatelet effects of clopidogrel. Twenty-nine healthy volunteers had platelet studies performed before and 1 week after 75 mg daily dosing of clopidogrel. Immature platelet fraction (IPF) was determined using an automated particle counter. Subjects were stratified into tertiles based on the IPF. Platelet studies included light transmission aggregometry (LTA), and vasodilator stimulated phosphoprotein phosphorylation (VASP-P) determined by platelet reactivity index (PRI). Baseline platelet aggregation responses to 2, 5 and 20 μM ADP, were similar in all three tertiles, however they were greater in the upper than in the lower tertile of immature platelets after clopidogrel in response to 5 μM ADP (54% vs. 23%, P = 0.02), with concordant trends for the other two concentrations. PRI was also greater in the upper tertile after clopidogrel (71.2% vs. 57.8%, P = 0.04). The frequency of clopidogrel hyporesponsiveness (aggregation >50% in response to 5 μM of ADP) was also higher in the upper tertile when compared to lower tertile, (60%) versus (10%) respectively (P = 0.02). [corrected]. Immature platelets measured using an automated method, are associated with impaired response to antiplatelet effects of clopidogrel.

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“…Studies where Sysmex XE-2100 IPF was compared with reference flow cytometric retPLT indicated low or moderate correlations [65,66]. Remarkably, the coefficients of correlation differed between patients groups, from no correlation in healthy individuals to relatively high correlations in patients with thrombocytopenia due to peripheral destruction [66,67].…”

Section: Comparison Of Reticulated and Immature Platelet Methodsmentioning

confidence: 99%

Reticulated platelets: analytical aspects and clinical utility

Hoffmann

2014

Clinical Chemistry and Laboratory Medicine (CCLM)

136

147

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Reticulated platelets are immature platelets circulating in blood; they reflect the activity of megakaryopoiesis in the bone marrow. Therefore, they can be used as a non-invasive test in patients with thrombocytopenia in various clinical conditions. The preferred method of analysis is by flow cytometry. However, there is an evident lack of analytical standardization, making it difficult to compare results obtained in different laboratories. Currently, two types of hematology analyzers are on the market offering fully automated measurement of reticulated or immature platelets: the high end analyzers manufactured by Sysmex (XE-and XN-series) and Abbott (CELL-DYN Sapphire). Although the methods are essentially different and cannot be used interchangeably, both have been proven to have clinical utility. Reticulated or immature platelet assays are useful for the differential diagnosis of thrombocytopenia and for monitoring bone marrow recovery after chemotherapy or stem cell transplantation. These assays may aid clinicians in platelet transfusion decisions when recovery from thrombocytopenia is imminent. In addition, preliminary findings indicate that there is a rationale for reticulated or immature platelets for risk stratification in acute coronary syndromes and for monitoring the effect of treatment with antiplatelet drugs in patients with coronary artery diseases. The aim of this paper is to present the present technology available for measuring reticulated platelets as well as an overview of the current status of clinical application. This overview also indicates that more research is needed before reticulated or immature platelet assays can be applied in other clinical conditions than thrombocytopenia and after transplantation.

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Comparison of an Immature Platelet Fraction and Reticulated Platelet in Liver Cirrhosis

Cited by 12 publications

References 14 publications

Immature Platelet Fraction: Establishment of a Reference Interval and Diagnostic Measure for Thrombocytopenia

Immature Platelet Fraction: Establishment of a Reference Interval and Diagnostic Measure for Thrombocytopenia

Immature platelet fraction (IPF) determined with an automated method predicts clopidogrel hyporesponsiveness

Reticulated platelets: analytical aspects and clinical utility

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