Comparison of an enzyme-linked immunoassay with an indirect hemagglutination assay for the detection of antibodies to cytomegalovirus

A competitive enzyme-linked immunosorbent assay (ELISA) for detecting cytomegalovirus (CMV) antibody was developed. The competitive ELISA was five times more sensitive than the complement fixation test (CFT) and twice as sensitive as indirect ELISA. Testing of paired sera from cardiac transplant patients taken before and after transplantation showed good correlation between results of competitive and indirect ELISA and CFT. The competitive ELISA was more successful than CFT or indirect ELISA in detecting passively acquired antibody, but detection of CMV antibody by competitive ELISA immediately after primary CMV infection was unreliable, possibly because of the high affinity of the monoclonal antibody chosen for the horseradish peroxidase conjugate. However, competitive ELISA may well prove to be more suitable than indirect ELISA for detecting CMV antibody in blood donations.

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Development of a competitive enzyme‐linked immunosorbent assay for detecting cytomegalovirus antibody

Wreghitt

Hicks

Gray

et al. 1986

Journal of Medical Virology

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A rapid chemiluminescent enzyme-linked immunosorbent assay for cytomegalovirus immunoglobulin G antibodies using instant photographic film

Nickless

Thorpe

Kricka

et al. 1985

Journal of Virological Methods

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Evaluation of CMV total antibody EIA

Chairez¹,

Cesario²,

Barrett³

et al. 1985

Diagnostic Microbiology and Infectious Disease

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Comparison of an enzyme-linked immunoassay with an indirect hemagglutination assay for the detection of antibodies to cytomegalovirus

Cited by 5 publications

References 22 publications

Development of a competitive enzyme‐linked immunosorbent assay for detecting cytomegalovirus antibody

Development of a competitive enzyme‐linked immunosorbent assay for detecting cytomegalovirus antibody

A rapid chemiluminescent enzyme-linked immunosorbent assay for cytomegalovirus immunoglobulin G antibodies using instant photographic film

Evaluation of CMV total antibody EIA

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