Comparison of an automated Borrelia indirect chemiluminescent immunoassay (CLIA) with a VlsE/C6 ELISA and Immunoblot

Riesbeck, Kristian; Hammas, Berit

doi:10.1007/s10096-007-0329-x

Cited by 10 publications

(10 citation statements)

References 9 publications

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“…In fact, our protocol recognized as positive only those serum samples positive by ELISA and Western blotting and two serum samples from subjects with early symptoms that give borderline reactions in serological tests. This result confirms the poor reliability of serological tests in early Borrelia infections (Riesbeck & Hammas, 2007). Moreover, the protocol we have described allowed us to identify at species level Borrelia DNA in human serum samples.…”

Section: Discussionsupporting

confidence: 76%

Detection of Borrelia burgdorferi sensu lato DNA by PCR in serum of patients with clinical symptoms of Lyme borreliosis

Santino

Berlutti

Pantanella

et al. 2008

FEMS Microbiology Letters

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Lyme borreliosis is a disease caused by spirochaetes belonging to the genospecies complex Borrelia burgdorferi sensu lato (s.l.) transmitted by Ixodes ticks. At present, serology remains the main diagnostic tool for laboratory diagnosis of Lyme borreliosis. Recently, the PCR technique has been applied for diagnosis of B. burgdorferi s.l., but, until now, a reliable, easy-to-perform and sensitive method has not been described. Here we present a new PCR-based method for the detection of both B. burgdorferi s.l. and Borrelia genospecies DNAs in serum samples collected from patients showing Lyme disease symptoms. Of 265 serum samples of patients included in this study, 7.5% were positive, 1.9% was borderline and 90.6% were negative for antibodies against B. burgdorferi by enzyme-linked immunosorbent assay and Western blotting. The B. burgdorferi s.l. 16S rRNA gene was detected by PCR in all serum-positive and in two borderline samples. None of the serum-negative samples nor serum samples collected from healthy subjects gave positive PCR reactions. Of PCR-positive serum samples, 50% gave a positive reaction for Borrelia afzelii, 18% for Borrelia garinii and 23% for two Borrelia species. Two samples (9%) were not identified to species level. The new protocol could be considered to be reliable as neither false-positive nor false-negative reactions were recorded, and to be sensitive as it detects DNA from one bacterial cell.

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Section: Discussionsupporting

confidence: 76%

Detection of Borrelia burgdorferi sensu lato DNA by PCR in serum of patients with clinical symptoms of Lyme borreliosis

Santino

Berlutti

Pantanella

et al. 2008

FEMS Microbiology Letters

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“…Our data also demonstrate noticeable differences of serum titers between ELISA and the CLIA assays used. These findings are in line with other studies on ELISA and CLIA assays showing that these assays have discrepancies in quantitative measurement but sound overall analytical agreement [10][11][12]. Moreover, recent data also suggest that even titers of different quantitative ELISA assays cannot be compared without further consideration [13,14].…”

Section: Discussionsupporting

confidence: 89%

Quantitative serological antibody testing for suspected neuroborreliosis

et al. 2020

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Objective To assess the importance of serum IgG/IgM antibody titers for the differentiation of Lyme neuroborreliosis (LNB) from its mimics. Method This was a retrospective, cross-sectional study conducted at two German neurological centers. Serological parameters (ELISA or CLIA analysis) and clinical presentation of 28 patients with definite LNB were compared to those of 36 patients with neurological symptoms mimicking LNB (mimics). Analysis was performed using receiver operating characteristic (ROC) and binary logistic regression. Results Elevated IgG-titers had a high sensitivity for neuroborreliosis in both centers (0.95 and 1.0). The optimal cutoffvalues were set to 26.35 in center A (ELISA), and 64.0 in center B (CLIA). Diagnostic specificity was 0.41 and 0.89 in this constellation. Elevated IgM-titers showed a high diagnostic specificity for a cutoff at 68.10 (A) and 47.95 (B) (0.93 and 0.89). Sensitivity was 0.45 and 0.5. Overall diagnostic accuracy was low in both centers (A: IgG AUC = 0.665, IgM AUC = 0.629; B: IgG AUC = 0.917, IgM AUC = 0.556). In logistic regression of antibody titers and clinical measures, prediction of LNB was significantly better than the "null hypothesis". Clinical measures showed the highest odds ratio. Conclusion Data show that in addition to the clinical presentation of patients with symptoms suggesting central or peripheral nervous system manifestation, serum IgG-and IgM-titers help to identify LNB-patients. The results should guide physicians counseling patients with suspected LNB about further diagnostic steps and treatment.

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“…Nevertheless, the flagellar antigen had higher specificity in this endemic population in analysis of the single first sample. Concerning the C6 assay, a high rate of seropositivity in both blood donors and consecutive routine specimens has been reported previously (13,29). The flagellar IgG assay and the C6 assays were chosen because they were routinely used by the laboratories in the region.…”

Section: Discussionmentioning

confidence: 62%

“…The C6 assay does not distinguish between IgG and IgM antibodies. Both assays have been described in previous publications from Scandinavian studies (9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19). The C6 assay was implemented on a Bio-Rad Coda microplate processor, and the IDEIA ELISA was performed manually.…”

Section: Methodsmentioning

confidence: 99%

Study of a Cohort of 1,886 Persons To Determine Changes in Antibody Reactivity to Borrelia burgdorferi 3 Months after a Tick Bite

Dessau¹,

Fryland²,

Wilhelmsson³

et al. 2015

Clin. Vaccine Immunol.

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Lyme borreliosis is a tick-borne disease caused by the bacterium Borrelia burgdorferi. The most frequent clinical manifestation is a rash called erythema migrans. Changes in antibody reactivity to B. burgdorferi 3 months after a tick bite are measured using enzyme-linked immunosorbent assays (ELISAs). One assay is based on native purified flagellum antigen (IgG), and the other assay is based on a recombinant antigen called C6 (IgG or IgM). Paired samples were taken at the time of a tick bite and 3 months later from 1,886 persons in Sweden and the Åland Islands, Finland. The seroconversion or relative change is defined by dividing the measurement units from the second sample by those from the first sample. The threshold for the minimum level of significant change was defined at the 2.5% level to represent the random error level. The thresholds were a 2.7-fold rise for the flagellar IgG assay and a 1.8-fold rise for the C6 assay. Of 1,886 persons, 102/101 (5.4%) had a significant rise in antibody reactivity in the flagellar assay or the C6 assay. Among 40 cases with a diagnosis of Lyme borreliosis, the sensitivities corresponding to a rise in antibodies were 33% and 50% for the flagellar antigen and the C6 antigen, respectively. Graphical methods to display the antibody response and to choose thresholds for a rise in relative antibody reactivity are shown and discussed. In conclusion, 5.4% of people with tick bites showed a rise in Borrelia-specific antibodies above the 2.5% threshold in either ELISA but only 40 (2.1%) developed clinical Lyme borreliosis. Borrelia burgdorferi sensu lato is transmitted by hard ticks (Ixodes ricinus or Ixodes persulcatus) throughout Europe, especially in the temperate climate zone. Both the vector and the bacterium are prevalent in southern Scandinavia, and ticks frequently attach to humans and occasionally transmit B. burgdorferi (1-4). Lyme borreliosis (LB) is also common in parts of the United States. The term B. burgdorferi sensu lato denotes a species complex belonging to the genus Borrelia and is herein designated simply B. burgdorferi or Borrelia. The most frequent species infecting humans in Europe are B. afzelii, B. garinii, and B. burgdorferi sensu stricto. In the United States, the only prevalent species is B. burgdorferi sensu stricto. The infections may involve the nervous system, the skin, the joints, and the heart. The most frequent manifestation is a characteristic skin lesion called erythema migrans (EM).This study was based on data from the tick-borne diseases (TBD) STING study (www.stingstudien.se). Ticks that had bitten humans in the Åland Islands, Finland, and different parts of Sweden were collected and analyzed at Linköping University (2-5). The focus of this study was to analyze the differences in the measurements of B. burgdorferi-specific antibodies in the paired samples and propose methods for detecting changes in antibody reactivity. Although the concept of antigen-induced stimulation and seroconversion has broad application in diagnostic medicine, epidem...

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Comparison of an automated Borrelia indirect chemiluminescent immunoassay (CLIA) with a VlsE/C6 ELISA and Immunoblot

Cited by 10 publications

References 9 publications

Detection of Borrelia burgdorferi sensu lato DNA by PCR in serum of patients with clinical symptoms of Lyme borreliosis

Detection of Borrelia burgdorferi sensu lato DNA by PCR in serum of patients with clinical symptoms of Lyme borreliosis

Quantitative serological antibody testing for suspected neuroborreliosis

Study of a Cohort of 1,886 Persons To Determine Changes in Antibody Reactivity to Borrelia burgdorferi 3 Months after a Tick Bite

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