Comparison of Amino Acid Separations on High Performance Silica Gel, Cellulose, and C-18 Reversed Phase Layers and Application of HPTLC to the Determination of Amino Acids in <i>Biomphalaria Glabrata</i> Snails

Norfolk, E.; Khan, Shahinul Haque; Fried, Bernard; Sherma, Joseph

doi:10.1080/10826079408013765

Cited by 14 publications

(4 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, thin-layer chromatography (TLC) on glass plates coated with 0.25-mm-thick silica gel G 60 F254 with a preconcentration zone (Merck; Darmstadt, Germany) was used to separate the amino acids as described by Norfolk et al [40]. The mobile phase consisted of sec-butanol, water, and acetic acid (3:1:1 v/v ).…”

Section: Methodsmentioning

confidence: 99%

[(Methyl)1-11C]-Acetate Metabolism in Hepatocellular Carcinoma

et al. 2010

View full text Add to dashboard Cite

Purpose Studies have established the value of [(methyl)1-11C]-acetate ([11C]Act) combined with 2-deoxy-2[18F]fluoro-d-glucose (FDG) for detecting hepatocellular carcinoma (HCC) using positron emission tomography (PET). In this study, the metabolic fate of [11C]Act in HCC was characterized. Methods Experiments with acetic acid [1-14C] sodium salt ([14C]Act) were carried out on WCH-17 cells and freshly derived rat hepatocytes. PET scans with [11C]Act were also carried out on woodchucks with HCC before injection of [14C]Act. The radioactivity levels in different metabolites were quantified with thin-layer chromatography. Results In WCH-17 cells, the predominant metabolite was phosphatidylcholine (PC). Regions of HCCs with the highest [11C]Act uptake had higher radioactivity accumulation in lipid-soluble compounds than surrounding hepatic tissues. In those regions, PC and triacylglycerol (TG) accumulated more radioactivity than in surrounding hepatic tissues. Conclusions High [11C]Act uptake in HCC is associated with increased de novo lipogenesis. PC and TG are the main metabolites into which the radioactive label from [11C]Act is incorporated in HCC.

show abstract

Section: Methodsmentioning

confidence: 99%

[(Methyl)1-11C]-Acetate Metabolism in Hepatocellular Carcinoma

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Chromatograms sprayed with ninhydrin (0.03 g ninhydrin in 100 ml of n-butanol plus 3 ml of glacial acetic acid) show air-sensitivity, stability, and color differentiation in comparison with different recipes of ninhydrin and fluorescamine sprays (Norfolk et al 1994). Another possibility for detection of amino acids was also described (Devani et al 1991;Laskar et al 1991;Laskar and Basak 1990a, b;Sinhababu et al 1994).…”

Section: Detection and Identification Of Biomolecules In Tlcmentioning

confidence: 97%

Basic Principles of Planar Chromatography and Its Potential for Hyphenated Techniques

Tuzimski

2010

High-Performance Thin-Layer Chromatography (HPTLC)

View full text Add to dashboard Cite

Sample preparation, detection, identification, and quantitative determination of biomolecules are presented in this chapter. Advantages of planar chromatography and the basic principles (chambers, sample application, and chromatogram development) are also described. Rapid detection of biomolecules plays a strategical role in their investigation. Hyphenated techniques such as planar chromatography coupled to UV diode array detection and to mass spectrometry provide on-line extensive structural information on the metabolites prior to their isolation. In this chapter, the combination of thin-layer chromatography (TLC) with biomolecules specific detection by diode array scanning (DAD), mass spectrometry (MS), nuclear magnetic resonance (NMR), and Fourier transform infrared spectroscopy (TLC-FTIR) is discussed. In the last part of the chapter, the reader will gain useful information about a recent method of planar chromatography -multidimensional planar chromatography (MD-PC) and information on application of different modes of multidimensional planar chromatography and combination of this technique with diode array detection (MD-PC-DAD) and HPLC-DAD for separation, detection, and qualitative and quantitative determination of biomolecules. Planar chromatography as a pilot technique for HPLC is also described.Biomolecules are widespread throughout the world. It is an organic molecule produced by a living organism, including large polymeric molecules such as proteins, polysaccharides, and nucleic acids as well as small molecules such as a primary metabolites, secondary metabolites, and natural products. Many sample-preparation techniques are used in biomolecule analysis; the method selected depends on the

show abstract

“…Results of high-performance silica gel, cellulose and RP-bonded silica gel for eighteen amino acids were compared with previous reports on IEC, RP-LC and paper chromatography. Determination was by scanning densitometry of the product (presumably 37) obtained after applying ninhydrin 141,142 . Determination of amino acids in pig plasma by IEC and detection by the ninhydrin method is influenced by the protein and lipophilic compounds present, causing lower resolution.…”

Section: Underivatized Analytesmentioning

confidence: 99%