Comparison of amino acid compositions of peptides eluted from HLA-B27 molecules of healthy individuals and patients with ankylosing spondylitis

Stodůlková, Eva; Pohl, Jan; Man, Petr; Votruba, J.; Capková, J; Sedláčková, Marie; Ivăsková, E; Iványi, P; Flieger, Miroslav

doi:10.1016/j.imlet.2005.10.017

Cited by 5 publications

(3 citation statements)

References 38 publications

(73 reference statements)

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“…In addition to Plasmodium, many other pathogens, such as Babesia gibsoni (56), Entamoeba histolytica (57), Pneumocystis carinii, and Mycobacterium tuberculosis (58), also have immunogenic glutamate-rich proteins, and some of them were evaluated as new diagnostic tools or vaccine candidates. Additionally, many allergens from plants (59)(60)(61) and some self-antigens that lead to autoimmune disease (62,63) were also glutamate-rich antigens. Thus, it is likely that there are many glutamate-rich proteins that are highly immunogenic in various pathogen species, especially in parasites.…”

Section: Discussionmentioning

confidence: 99%

Low-Complexity Repetitive Epitopes of Plasmodium falciparum Are Decoys for Humoural Immune Responses

Hou

Jiang

et al. 2020

Front. Immunol.

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Induction of humoural immunity is critical for clinical protection against malaria. More than 100 malaria vaccine candidates have been investigated at different developmental stages, but with limited protection. One of the roadblocks constrains the development of malaria vaccines is the poor immunogenicity of the antigens. The objective of this study was to map the linear B-cell epitopes of the Plasmodium falciparum erythrocyte invasion-associated antigens with a purpose of understanding humoural responses and protection. We conducted a large-scale screen using overlapping peptide microarrays of 37 proteins from the P. falciparum parasite, most of which are invasion-associated antigens which have been tested in clinical settings as vaccine candidates, with sera from individuals with various infection episodes. Analysis of the epitome of the antigens revealed that the most immunogenic epitopes were predominantly located in the low-complexity regions of the proteins containing repetitive and/or glutamate-rich motifs in different sequence contexts. However, in vitro assay showed the antibodies specific for these epitopes did not show invasion inhibitory effect. These discoveries indicated that the low-complexity regions of the parasite proteins might drive immune responses away from functional domains, which may be an instructive finding for the rational design of vaccine candidates.

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Section: Discussionmentioning

confidence: 99%

Low-Complexity Repetitive Epitopes of Plasmodium falciparum Are Decoys for Humoural Immune Responses

Hou

Jiang

et al. 2020

Front. Immunol.

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“…36 HLA B27 molecules have also been proved to be rich in proline. 37 This might potentially be another source of the higher blood proline content in the AS population. Hyperhomocysteinemia is usually associated with rheumatic diseases, partially due to vitamin dysmetabolism.…”

Section: Biological Significance Of the Altered Metabolites In Asmentioning

confidence: 99%

Integrated GC–MS and LC–MS plasma metabonomics analysis of ankylosing spondylitis

Gao

Lü

Zhang

et al. 2008

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Ankylosing spondylitis (AS) is a chronic inflammatory arthritis that predominantly affects the axial skeleton in adolescent patients. The natural history of the disease remains poorly characterized. In this study, we combined GC-MS and LC-MS techniques to evaluate the major metabolic changes in the plasma of AS patients in view of metabonomics. Univariate and multivariate analysis were employed for altered metabolite comparison and pattern recognition. Application of supervised partial least-squares discrminant analysis to either GC-MS or LC-MS data allowed accurate discrimination of AS patients from normal controls, demonstrating its potential diagnostic utilization. In addition, AS patients presented elevated plasma concentrations of proline, glucose, phosphate, urea, glycerol, phenylalanine and homocysteine but reduced levels of phosphocholines, tryptophan and a bipeptide - phenylalanyl-phenylalanine. In the context of their involved metabolic pathways, the identified metabolites were discussed accordingly. This investigation primarily proved that integrated chromatography-mass spectrometry and integrated uni- and multi-variate statistical analysis facilitated metabonomics to be a more promising tool in disease research.

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“…7 According to reports, the immune effects that occurred in collagen tissue may lead to excessive breakdown of collagen components, thereby increasing the circulating proline in the blood. 9 It is also worth noticing that HLA-B27 molecules have been proven to be rich in proline, 10 and the HLA-B27 positivity has been widely used in clinical practice to identify SpA. 11,12 What's more, unpaired cysteine residues in HLA antigens can be directly or indirectly modified by homocysteine and then subsequently disrupted by abnormal autoimmune reactions, which is also considered a mechanism of AS associated with HLA-B27.…”

Section: Introductionmentioning

confidence: 99%

Identification of a Novel Gene Expression Signature Associated with Amino Acid Metabolism (AAM) in Ankylosing Spondylitis (AS)

Liu,

Peng

2024

IJGM

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Background: Studies have proven that amino acid metabolism (AAM) plays an important role in ankylosing spondylitis (AS). Therefore, this study identified key AAM-related genes (AAMRGs) for the diagnosis and prediction of AS. Methods: Firstly, the differentially expressed genes (DEGs) were identified between AS and normal groups in the GSE25101 and GSE73754 datasets downloaded from the Gene Expression Omnibus (GEO) database, and they were intersected to get common DEGs (Co-DEGs). Weighted Gene Co-expression Network Analysis (WGCNA) was used to identify AS and AAM score-related genes (AS-AAMSRGs). Then, AAM related DEGs (AAMR DEGs) were acquired by intersection of Co-DEGs and AS-AAMSRGs. Moreover, the least absolute shrinkage and selection operator (LASSO) was implemented on AAMR DEGs to identify diagnostic genes, and Gene Set Enrichment Analysis (GSEA) was used to explore the functional pathways of diagnostic genes. By screening differential immune cells, the correlation between differential immune cells and diagnostic genes was further analyzed. Finally, miRNA-mRNA networks were constructed and drug prediction analysis was performed. Results: By overlapping to obtain three AAMR DEGs (TP53INP1, TUBB and RBM47). The results of nomogram and decision curve analysis (DCA) suggested that three AAMR DEGs had diagnostic value for AS and significantly enriched to neutrophil activation, neutrophil degranulation. The proportion of eight kinds of immune cells in AS and normal groups was significantly different, such as activated dendritic cell, CD56 bright natural killer cell, effector memory CD4 T cell. In the miRNA-mRNA regulatory networks, three miRNAs (has-miR-429, has-miR-200c-3p, has-miR-200b-3p) could regulate TP53INP1 and TUBB. There was only one miRNA (has-miR-122-5p) could regulate RBM47. Finally, 51 target drugs (such as colchicine, vinblastine, vincristine) were associated with TUBB. Conclusion: TP53INP1, TUBB and RBM47 might play key roles in AS and could be used as potential biomarkers of AS.

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Comparison of amino acid compositions of peptides eluted from HLA-B27 molecules of healthy individuals and patients with ankylosing spondylitis

Cited by 5 publications

References 38 publications

Low-Complexity Repetitive Epitopes of Plasmodium falciparum Are Decoys for Humoural Immune Responses

Low-Complexity Repetitive Epitopes of Plasmodium falciparum Are Decoys for Humoural Immune Responses

Integrated GC–MS and LC–MS plasma metabonomics analysis of ankylosing spondylitis

Identification of a Novel Gene Expression Signature Associated with Amino Acid Metabolism (AAM) in Ankylosing Spondylitis (AS)

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