Comparison of ameliorative effects ofTaraxacum syriacumand N-acetylcysteine against acetaminophen-induced oxidative stress in rat liver and kidney

Abstract

Taraxacum syriacum (TS) with natural antioxidant and pharmacological activities may be considered for treatment of oxidative stress induced by acetaminophen (APAP). The aim of the present study was to evaluate the ameliorative effects of the ethanol extract of TS root against hepatorenal toxicity induced by APAP in comparison to N-acetylcysteine (NAC) as a standard drug. Thirty male Wistar rat were randomly divided into five groups. Control group; APAP (1g/kg) group; APAP-NAC (160 mg/kg) group and APAP-TS100 and APAP-TS200 groups: APAP plus 100 and 200 mg/kg of TS extract, respectively. After 7 days treatment, serum and liver and kidney tissues were prepared and evaluated. TS extract ameliorated the increased lipid peroxidation level and decreased antioxidant enzymes activities and glutathione level in liver and kidney of APAP-treated rats. Moreover, treatment with the TS extract caused significant reduction in the histopathological damages and high levels of serum biochemical markers of hepatic and renal functions after APAP treatment. This study suggests that the extract of TS roots has dose-dependent ameliorative effect against APAP-induced oxidative damage in liver and kidney due to its free radical scavenging and antioxidant properties. The overall efficacy of the extract at 200 mg/kg dose is comparable with NAC.

Allicin and Omega-3 fatty acids attenuates acetaminophen mediated renal toxicity and modulates oxidative stress, and cell apoptosis in rats

“…These changes in the kidney structure may have been responsible for the lipid peroxidation induced by APAP. These results are consistent with previous studies that described renal histological changes after APAP administration (El-Maddawy and El-Sayed 2018 ; Reshi et al 2020 ; Eshrati et al 2021 ; Wans et al 2021 ; Dallak et al 2022 ; Sohail et al 2022 ). However, treatment with ALC and/or O3FA preserved the normal structure of renal tubules, with only a few mildly dilated glomeruli spaces in some fields.…”

“…Our study showed that APAP overdose led to an increase in renal MDA levels and a significant inhibition of major renal antioxidant enzymes (GSH, SOD, and CAT) activities. The decrease in SOD, CAT, and GSH levels, along with an increase in MDA levels, is consistent with previous research on APAP-induced nephrotoxicity (Canayakin et al 2016 ; Abdeen et al 2019 ; Eshrati et al 2021 ; Wans et al 2021 ).…”

“…However, treatment with O3FA improved renal function and had renoprotective effects by normalizing these biomarker levels. The renal damage caused by APAP toxicity is primarily associated with the overproduction of ROS and a reduction in antioxidant capacity, as indicated by several studies (Hasanein and Sharifi 2017 ; Eraky and El-Magd 2020 ; Eshrati et al 2021 ). Allicin treatment also resulted in a significant reduction in serum creatinine and urea levels in cisplatin-intoxicated rats, according (Abdel-Daim et al 2019 ).…”

Tissues toxicity attenuation by vitamin E on oxidative damage induced by diazinon

“…The increase in the activities of ALT, AST, LDH, ALP and CK enzymes and urea, uric acid and creatinine levels are used as important diagnostic tool for cellular damage and cytotoxicity of toxic agents [21][22][23]. In this study, DZN significantly increased plasma AST, ALT, ALP, CK and LDH activities and urea, uric acid and creatinine levels compared with those of the control group (p<0.05) (Figure 1 and 2).…”

“…MDA is a stable metabolite of the free radical-mediated lipid peroxidation cascade and it is known as a marker of oxidative stress [22,44]. Our results showed a significant increase in the level of MDA in all tissues except spleen (Figure 4), which might have resulted from oxidative damage induced by DZN, might be due to its lipophilicity, whereby it could penetrate easily to the cell membrane and caused membrane lipid peroxidation [9].…”