Comparison of algal assay systems for detecting waterborne herbicides and metals

Turbak, S.C.; Olson, Susan B.; McFeters, Gordon A.

doi:10.1016/0043-1354(86)90219-8

Cited by 53 publications

(12 citation statements)

References 19 publications

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“…Nevertheless, it is unclear whether the differences in sensitivity among various algal taxas and within individual species reported in the current literature are caused by differences among the various biotic and abiotic factors in standard operating procedures Janssen and Heijerick (2003). Such variability can be verified in data obtained by different authors Masutti (2004); Rodgher and Espíndola (2008); Rojícková and Maršálek (1999); Turbak et al (1986) (Table 4). …”

Section: Discussionmentioning

confidence: 77%

A study of the effects of chromium exposure on the growth of Pseudokirchneriella subcapitata (Korshikov) hindak evaluated by Central Composite Design and Response Surface Methodology

et al. 2010

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The aim of this study was to evaluate the effects of chromium exposure on the growth of P. subcapitata using the Central Composite Design (CCD) and Response Surface Methodology (RSM). The highest values for algal density and biomass were obtained in the longest exposure times and for the lowest chromium concentrations. The CCD used for the analysis of treatment combinations showed that a second order polynomial regression model was in good agreement with experimental results, with R (2) = 81.50 and 89.90; for algal density and biomass (p < 0.05), respectively. Only the exposure time was significant for algal density. For chlorophyll, in contrast, the exposure time, chromium concentration and their interaction significantly affected the growth of P. subcapitata. The findings confirmed the sensitivity of P. subcapitata to chromium (VI), which makes it a suitable bioindicator of environmental contamination for this metal.

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Section: Discussionmentioning

confidence: 77%

A study of the effects of chromium exposure on the growth of Pseudokirchneriella subcapitata (Korshikov) hindak evaluated by Central Composite Design and Response Surface Methodology

et al. 2010

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“…They show high sensitivity towards toxicants and can thus reveal any change in water quality (Turbak et al 1986;Walsh and Merrill 1984). Euglena gracilis is a green unicellular alga inhabiting many freshwater ecosystems.…”

Section: Introductionmentioning

confidence: 98%

A fast algal bioassay for assessment of copper toxicity in water using Euglena gracilis

Ahmed

Häder

2010

J Appl Phycol

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A rapid, sensitive algal bioassay was investigated to monitor copper toxicity using photosynthetic activity and motility parameters as end points in the photosynthetic flagellate Euglena gracilis. Effects on motility parameters were determined using the automatic bioassay ECOTOX. Photosynthetic efficiency was measured using chlorophyll fluorescence by means of a fast, noninvasive pulse amplitude modulated fluorometer. These parameters were assessed regarding their effectiveness as end points for short-(0-24 h) and long-term (1-5 days) toxicity tests. The model organism showed significant responses to the tested concentrations at both motility and photosynthesis examined levels. EC 50 values for movement parameters immediately after the cells were mixed with copper are 19.09 (300.4 μM), 20.4 (312 μM) and 23.09 (363.35 μM)mg L −1 for motility, rvalue and velocity, respectively. The current study proofs that Euglena is a convenient biotest organism where motility parameters show rapid sensitive responses to copper (direct exposure to copper). Also, the photosynthetic parameters appear to be appropriate for acute and chronic toxicity tests for higher and lower copper concentrations, respectively.

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“…Many organisms, belonging to different groups such as algae, fish, and invertebrates, are used in bioassays. For aquatic bioassays, algae seem to be more suitable organisms because they are very sensitive to different pollutants and occur abundantly in aquatic ecosystems (Ahmed and Häder 2010a;Turbak et al 1986;Danilov and Ekelund 2000). Several studies reveal that algae are more sensitive than invertebrates and fish to different water pollutants and industrial sewage (Lewis 1995;Paixão et al 2008;Blinova 2004;Walsh and Garnas 1983).…”

Section: Introductionmentioning

confidence: 98%

Sensitivity of various parameters in Euglena gracilis to short-term exposure to industrial wastewaters

Azizullah

Richter

Häder³

2011

J Appl Phycol

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Due to the presence of various potential pollutants industrial wastewaters pose considerable threats to natural waters and make it unfit for the aquatic biota. Analysis of wastewaters by chemical methods is a common practice; however, it does not reflect the toxic effects on living organism. Therefore, bioassessment is necessary for monitoring of wastewater quality. In the present study, the toxic effects of wastewater samples from different industries were evaluated using Euglena gracilis as a biotest organism. Various parameters of the freshwater flagellate E. gracilis like motility, swimming velocity, cell shape, gravitactic orientation (using the automatic biotest ECOTOX) and photosynthetic efficiency (using a pulse amplitude modulated fluorometer) were used as end points for toxicity assessment. In addition, the samples were analysed for some ecologically important physicochemical properties. With some exceptions, most of the physicochemical properties of the tested samples were within the acceptable range of national environmental quality standards for municipal and industrial effluents. However, all the water samples adversely affected different parameters in E. gracilis. This study led to the conclusion that different toxic substances present in wastewater, even at low concentrations, can be a possible threat to aquatic biota. The results of this study prove that ECOTOX is a sensitive, easy, and fast bioassay for monitoring of water and wastewater quality. Gravitactic orientation and cell compactness of E. gracilis were the most sensitive parameters to wastewater toxicity.

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Comparison of algal assay systems for detecting waterborne herbicides and metals

Cited by 53 publications

References 19 publications

A study of the effects of chromium exposure on the growth of Pseudokirchneriella subcapitata (Korshikov) hindak evaluated by Central Composite Design and Response Surface Methodology

A study of the effects of chromium exposure on the growth of Pseudokirchneriella subcapitata (Korshikov) hindak evaluated by Central Composite Design and Response Surface Methodology

A fast algal bioassay for assessment of copper toxicity in water using Euglena gracilis

Sensitivity of various parameters in Euglena gracilis to short-term exposure to industrial wastewaters

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