Comparison of agglutination, complement fixation and immunofluorescence tests in <i>Campylobacter jejuni</i> infections

Watson, K. C.; Kerr, E. J. C.

doi:10.1017/s0022172400070042

Cited by 12 publications

(5 citation statements)

References 5 publications

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“…Immunization. Young adult New Zealand white rabbits were prebled and then immunized with 10 ml of antigen given as five separate doses of 2 ml each as follows: intradermally on day (37,38). e One of the original four human isolates reported by King (14).…”

Section: Methodsmentioning

confidence: 99%

Serogroups of Campylobacter jejuni, Campylobacter coli, and Campylobacter fetus defined by direct immunofluorescence

Hébert¹,

Hollis²,

Weaver³

et al. 1983

J Clin Microbiol

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Rabbits were inoculated with whole, formalinized Campylobacter jejuni, C. coli, and C. fetus cells; the C. jejuni and C. coli immunogens were identified by their DNA relatedness at the species level to the type strains of C. jejuni or C. coli. The designation C. coli was not used among the other C. jejuni strains; they were classified as hippurate-positive or hippurate-negative C. jejuni. Immunoglobulin G was isolated from the antisera and labeled with fluorescein isothiocyanate. These conjugates defined 10 serogroups of C. jejuni, 2 serogroups of C. coli, and 2 serogroups of C. fetus. Of the 316 strains of C. jejuni tested, 258 (82%) were groupable; 173 were single-serogroup strains, and 85 were mutliple-serogroup strains. Of the 226 strains in C. jejuni serogroups, 223 (98.7%) were hippurate positive; of the 27 strains in C. coli serogroups, 26 (96.3%) were hippurate negative. Five strains were equally reactive in immunofluorescent staining with a conjugate for a C. jejuni serogroup and a conjugate for a C. coli serogroup. A total of 58 strains of C. jejuni were ungroupable: 33 (13%) of the 259 hippurate-positive strains and 25 (44%) of the 57 hippurate-negative strains. All 121 strains of C. fetus tested were groupable as A, B, or A:B. The 14 conjugates used to define serogroups of C. jejuni, C. coli, and C. fetus reacted with the flagella but not the cells of other Campylobacter species and were negative on 256 other bacteria from 21 genera.

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Section: Methodsmentioning

confidence: 99%

Serogroups of Campylobacter jejuni, Campylobacter coli, and Campylobacter fetus defined by direct immunofluorescence

Hébert¹,

Hollis²,

Weaver³

et al. 1983

J Clin Microbiol

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“…Agglutination and complement fixation (24,25) and immunofluorescence (4) tests have been used for serological diagnosis of C. jejuni infection, but these have been limited by low sensitivity or specificity or the need to use homologous isolates. Few attempts on an experimental basis have been made for the development of enzyme immunoassays for detecting antibody response to C. jejuni (3,9,10,22,23).…”

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confidence: 99%

Antibody Responses to Campylobacter Infections Determined by an Enzyme-Linked Immunosorbent Assay: 2-Year Follow-Up Study of 210 Patients

Strid¹,

Engberg²,

Larsen³

et al. 2001

Clin Diagn Lab Immunol

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An enzyme-linked immunosorbent assay (ELISA) was adapted to measure immunoglobulin G (IgG), IgM, and IgA classes of human serum antibody to Campylobacter jejuni and Campylobacter coli. Heat-stable antigen, a combination of C. jejuni serotype O:1,44 and O:53 in the ratio 1:1, was used as a coating antigen in the ELISA test. A total of 631 sera from 210 patients with verified Campylobacter enteritis were examined at various intervals after infection, and a control group of 164 sera were tested to determine the cut-off for negative results. With a 90th percentile of specificity, IgG, IgM, and IgA showed a sensitivity of 71, 60, and 80%, respectively. By combining all three antibody classes, the sensitivity was 92% within 35 days after infection, whereas within 90 days after infection, a combined sensitivity of 90% was found (IgG 68%, IgM 52%, and IgA 76%). At follow-up of the patients, IgG antibodies were elevated 4.5 months after infection but exhibited a large degree of variation in antibody decay profiles. IgA and IgM antibodies were elevated during the acute phase of infection (up to 2 months from onset of infection). The antibody response did not depend on Campylobacter species or C. jejuni serotype, with the important exception of response to C. jejuni O:19, the serotype most frequently associated with Guillain-Barré syndrome. All of the patients infected with this serotype had higher levels of both IgM (P ‫؍‬ 0.006) and IgA (P ‫؍‬ 0.06) compared with other C. jejuni and C. coli serotypes.

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“…Several assays including agglutination (22,23), immunofluorescence (2), complement fixation (9, 10), and bactericidal (8) tests have been used for serological diagnosis of C. jejuni infection, but these have been limited by low sensitivity or specificity or the need to use homologous isolates. Agglutination, complement fixation, and bactericidal tests chiefly measure immunoglobulin M (IgM), another limitation.…”

mentioning

confidence: 99%

Human serum antibody response to Campylobacter jejuni infection as measured in an enzyme-linked immunosorbent assay

Blaser¹,

Duncan²

1984

Infect Immun

156

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An enzyme-linked immunosorbent assay was adapted to measure immunoglobulin A (IgA), IgG, and IgM classes of human serum antibody to Campylobacterjejuni. Sera were tested from healthy controls, from ill persons at various intervals after exposure to an epidemiologically implicated vehicle for Campylobacter sp. enteritis, from persons exposed to these same vehicles who remained well, and from persons who chronically drank raw milk. The major antigens in the C. jejuni acid-washed antigen preparations from three different strains all migrated at about 30,000 and 63,000. Persons with Campylobacter enteritis developed rising serum IgA, IgG, and IgM antibodies during the second week after infection; IgG and IgM elevations persisted longer than did IgA. Exposed persons who remained well showed similar, but lower, antibody rises. Chronic raw milk drinkers had elevated IgG levels, but not IgM or IgA levels, whether or not they were acutely exposed to an implicated vehicle.

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Comparison of agglutination, complement fixation and immunofluorescence tests in Campylobacter jejuni infections

Cited by 12 publications

References 5 publications

Serogroups of Campylobacter jejuni, Campylobacter coli, and Campylobacter fetus defined by direct immunofluorescence

Serogroups of Campylobacter jejuni, Campylobacter coli, and Campylobacter fetus defined by direct immunofluorescence

Antibody Responses to Campylobacter Infections Determined by an Enzyme-Linked Immunosorbent Assay: 2-Year Follow-Up Study of 210 Patients

Human serum antibody response to Campylobacter jejuni infection as measured in an enzyme-linked immunosorbent assay

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