Comparison of aerial and submerged spore properties for Trichoderma harzianum

Muñoz, Gastón

doi:10.1016/0378-1097(94)00474-6

Cited by 53 publications

(23 citation statements)

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“…Moreover, differences in hydrophobicity have been shown to affect the viability of Trichoderma harzianum. Aerially produced spores were highly hydrophobic and showed longer viability after storage than submerged spores [14]. Furthermore, most filamentous fungi remain entirely vegetative in submerged culture, which is consistent with the findings that differentiated structures are characteristic of aerial mycelium [11].…”

Section: Introductionsupporting

confidence: 83%

Impact of solid medium composition on the conidiation in Penicillium camemberti

Krasniewski¹,

Molimard²,

Féron³

et al. 2006

Process Biochemistry

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Section: Introductionsupporting

confidence: 83%

Impact of solid medium composition on the conidiation in Penicillium camemberti

Krasniewski¹,

Molimard²,

Féron³

et al. 2006

Process Biochemistry

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“…In contrast, conidia of the mycoparasite Coniothyrium minitans are hydrophobic, although in this case, conidial hydrophobicity decreases with culture age for some isolates (Smith et al, 1998). Similarly, a comparison of two cell types of Trichoderma harzianum, a potential biological control agent of phytopathogenic fungi, reveals that aerial conidia display higher UV resistance and longer viability, and are more hydrophobic than submerged conidia, which are hydrophilic (Munoz et al, 1995). Contact angle measurements, microbial adhesion to solvents and zeta potential determinations of blastospores of the entomopathogenic fungus P. fumosoroseus indicate that these cells have a hydrophilic, basic monopolar surface, and are negatively charged under neutral conditions (Dunlap et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Surface characteristics of the entomopathogenic fungus Beauveria (Cordyceps) bassiana

et al. 2007

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Marked differences in surface characteristics were observed among three types of single-cell propagules produced by the entomopathogenic fungus Beauveria bassiana. Atomic force microscopy (AFM) revealed the presence of bundles or fascicles in aerial conidia absent from in vitro blastospores and submerged conidia. Contact angle measurements using polar and apolar test liquids placed on cell layers were used to calculate surface tension values and the free energies of interaction of the cell types with surfaces. These analyses indicated that the cell surfaces of aerial conidia were hydrophobic, whereas those of blastospores and submerged conidia were hydrophilic. Zeta potential determinations of the electrostatic charge distribution across the surface of the cells varied from +22 to "30 mV for 16-day aerial conidia at pH values ranging from 3 to 9, while the net surface charge ranged from +10 to "13 mV for submerged conidia, with much less variation observed for blastospores, +4 to "4 mV, over the same pH range. Measurements of hydrophobicity using microbial adhesion to hydrocarbons (MATH) indicated that the surfaces of aerial conidia were hydrophobic, and those of blastospores hydrophilic, whereas submerged conidia displayed cell surface characteristics on the borderline between hydrophobic and hydrophilic. Insect pathology assays using tobacco budworm (Heliothis virescens) larvae revealed some variation in virulence among aerial conidia, in vitro blastospores and submerged conidia, using both topical application and haemocoel injection of the fungal cells.

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“…The analytical procedure for trehalose extraction and determination was the same as reported in [18]. Basically, it consisted of grinding 10 mg of lyophilized spores with glass beads, extracting the resulting powder twice with hot water and centrifugation at 1000 × g for 15 min.…”

Section: Trehalose Determinationmentioning

confidence: 99%

Viability of dry Trichoderma harzianum spores under storage

Pedreschi

Aguilera

1997

Bioprocess Engineering

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Spores of the potential biocontrol agent Trichoderma harzianum P1 were prepared without (M1) and with heat shock (40°C for 90 min) after fermentation (M2), filtered into a paste and dried over silica gel. M1 and M2 exhibited high viability (55%) and similar initial trehalose contents (4.0 and 5.4%, respectively) after slow drying. No significant differences in viability were found between treatments during storage for 110 days under different temperatures, T (8, 33 and 42°C) and water activities, a w (0.03, 0.33 and 0.75). Viability of spores, after storage at a w 0:03 were 100 and 70% for 8 and 33°C, respectively. During storage, decrease in trehalose content and viability was faster at a w 0:75 and 42°C. Loss of viability was modeled by a first order kinetic model depending on 1=T and a w . M2 (with heat shock) showed slightly higher trehalose contents than M1 which resulted in 100% viability after 52 days at 8°C. List of symbols a wwater activity , and parameters in GAB equation 1 , and parameters in kinetic equation AV t absolute viability of dried spores after time t AV 0 absolute viability of spores after drying CFU colony forming units k kinetic constant, function of T and a w days )1 k b y c constants in the GAB equation m moisture content g water/g dry spores m 0 monolayer value g water/g dry spores t storage time days TS total number of spores (conidia) V relative viability at certain time t V 0 relative viability at time zero

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Comparison of aerial and submerged spore properties for Trichoderma harzianum

Cited by 53 publications

References 0 publications

Impact of solid medium composition on the conidiation in Penicillium camemberti

Impact of solid medium composition on the conidiation in Penicillium camemberti

Surface characteristics of the entomopathogenic fungus Beauveria (Cordyceps) bassiana

Viability of dry Trichoderma harzianum spores under storage

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