Comparison of adsorption-extraction (AE) workflows for improved measurements of viral and bacterial nucleic acid in untreated wastewater

Akter, Jesmin; Smith, Wendy J.M.; Liu, Yawen; Kim, Ilho; Simpson, Stuart L.; Thai, Phong; Korajkic, Asja; Ahmed, Warish

doi:10.1016/j.scitotenv.2023.167966

Cited by 3 publications

(1 citation statement)

References 44 publications

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“…No. 14700-50-NF, Qiagen) did not guarantee a higher DNA yield when compared with PM1 used alone [ 3 , 31 ]. Furthermore, qPCR inhibition was observed in AWW1 and AWW2 when using extraction method EP9 and EP10.…”

Section: Discussionmentioning

confidence: 99%

Assessment of nucleic acid extraction protocols for antibiotic resistance genes (ARGs) quantification in aircraft wastewater

Smith,

Liu,

Simpson

et al. 2024

Hum Genomics

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This study evaluated ten nucleic acid extraction protocols (EP1 to EP10) for measuring five endogenous antibiotic resistance genes (ARGs) in four aircraft wastewater samples (AWW1 to AWW4). The targeted ARGs, including blaCTX-M, blaNDM-1, ermB, qnrS, and tetA, encompassed highly and minimally abundant ARGs. TetA and ermB were consistently detected across four aircraft wastewater samples using the DNeasy Blood and Tissue Kit and the AllPrep PowerViral DNA/RNA kit. QnrS displayed high detection rates with specific extraction protocols and aliquot volumes. Concentrations of ARGs varied across aircraft wastewater samples, with differing extraction protocols influencing quantitative results. The concentrations of tetA, ermB, and qnrS in AWW1 were distinct, while AWW2 to AWW4 exhibited a broader range for tetA, ermB, qnrS, blaCTX-M, and blaNDM-1. EP1 consistently produced the highest concentrations for several ARGs. Collective data analysis revealed varying ARG concentrations across the ten extraction protocols, suggesting the importance of careful extraction protocol selection in ARG monitoring in aircraft wastewater samples. Based on the results, we suggest that a small sample volume (as low as 0.2 mL) may be sufficient for ARG characterization in aircraft wastewater samples. The findings also emphasize the need for considering toilet paper removal without compromising nucleic acid extraction efficiency. The study highlights promising prospects for aircraft wastewater monitoring of ARGs, calling for further investigation into the import and spread of unique ARGs through transport hubs.

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Section: Discussionmentioning

confidence: 99%

Assessment of nucleic acid extraction protocols for antibiotic resistance genes (ARGs) quantification in aircraft wastewater

Smith,

Liu,

Simpson

et al. 2024

Hum Genomics

Self Cite

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Comparative analysis of membrane filter diameters for detection of selected viruses in wastewater samples

Solomon,

Idris,

Nwaubani

et al. 2024

Science of The Total Environment

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Comparative Inactivation of the RNA of the Delta and Omicron Variants of SARS-CoV-2 in Wastewater of Five Municipalities in Southeast Germany

Geissler,

Dumke

2024

Water

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During the COVID-19 pandemic, wastewater-based epidemiology has been extensively used as a helpful tool for evaluation of the epidemic situation in catchments of wastewater treatment plants. In this context, knowledge about the rate of virus inactivation in wastewater is important for characterization of the influence of retention times in sewers on virus concentrations, of the storage periods before analysis in the laboratory, on virus elimination during treatment and for modeling approaches. In the present study, we genotyped SARS-CoV-2 in the respiratory tract specimens of infected patients and spiked the raw wastewater of five treatment plants with aliquots of these samples. The test preparations were incubated under practically relevant temperature conditions (14 °C) over 31 days. The linear decay rates resulted in comparable T90 values (30.5 d) for the Delta and the Omicron BA1 variants of SARS-CoV-2 without differences in using the E or S gene of the virus as the target for amplification. In contrast, the origin of wastewater influenced the inactivation rate of both variants significantly, with the mean T90 values varying between 24.3 and 53.1 days in the wastewater from the five plants. The data suggest that the inactivation rate of SARS-CoV-2 in wastewater is more strongly determined by the composition of the water than by the virus variant.

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Comparison of adsorption-extraction (AE) workflows for improved measurements of viral and bacterial nucleic acid in untreated wastewater

Cited by 3 publications

References 44 publications

Assessment of nucleic acid extraction protocols for antibiotic resistance genes (ARGs) quantification in aircraft wastewater

Assessment of nucleic acid extraction protocols for antibiotic resistance genes (ARGs) quantification in aircraft wastewater

Comparative analysis of membrane filter diameters for detection of selected viruses in wastewater samples

Comparative Inactivation of the RNA of the Delta and Omicron Variants of SARS-CoV-2 in Wastewater of Five Municipalities in Southeast Germany

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