Comparison of acetyl bromide lignin with acid detergent lignin and Klason lignin and correlation with in vitro forage degradability

Fukushima, Romualdo Shigueo; Kerley, M. S.; Ramos, Maurivan Güntzel; Porter, Julie; Kallenbach, Robert L.

doi:10.1016/j.anifeedsci.2014.12.007

Cited by 54 publications

(51 citation statements)

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“…The NDF content observed in most grasses within each DAP was much lower than values reported in other grasses like Lilum sp. (Fukushima et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Effect of cutting time on agronomic and nutritional characteristics of nine commercial cultivars of Brachiaria grass compared with Napier grass during establishment under semi-arid conditions in Rwanda

Mutimura¹,

Ebong²,

Rao³

et al. 2017

Afr. J. Agric. Res.

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A study was conducted to identify the most productive cultivars and their cutting management to optimize nutrient productivity in semi-arid areas of Rwanda. Four cultivars of Brachiaria brizantha (Piatá, MG4, Marandú and Xaraes), two cultivars of Brachiaria humidicola (Llanero and Humidicola), two cultivars of Brachiaria hybrid (Mulato and Mulato II) and one cultivar of Brachiaria decumbens (Basilisk) were evaluated against Napier grass (Pennisetum purpureum) in an on-farm trial in a complete randomised block design with four replicates. Forage samples were collected at 60, 90 and 120 days after planting (DAP) and analysed for dry matter (DM), crude protein (CP), organic matter (OM) and neutral detergent fibre (NDF). The nutritional values were also estimated using in vitro gas production (IVGP) and its kinetic parameters, in vitro apparent degradable dry matter (ivADDM), digestible organic matter (DOM), metabolisable energy (ME), partitioning factor (PF) and degradable efficiency factor (DEF). For all cultivars and species, the DM, CP, OM, ivADDM and DOM increased from 60 to 90 DAP and declined thereafter. The NDF contents increased with increase in plant age. Grasses cut at 90 DAP had the highest gas production. The ME differed among grasses and DAP where Piatá had the highest. Furthermore, degradability parameters (A, B, C) and half time (T 1/2 ) differed among grasses and between cutting times. The PF and DEF were correlated with ME. The most promising cultivars were Basilisk, Marandú, Piatá and Mulato II because of their high in these nutritional characteristics.Key words: Chemical composition, metabolisable energy, degradability parameters, degradation efficiency factor. INTRODUCTIONIntensification of ruminant livestock production is gaining momentum in a number of sub-Saharan African countries due to increasing population pressure and decline in grazing areas (Thornton and Herrero, 2014). Even farmers with access to open grazing land experience frequent feed shortage due to climate change and variability (Chirat et al., 2014).Feeds and feeding, especially for dairy cows are a critical issue for smallholder farmers in Rwanda, mostly during the dry season (Klapwijk et al., 2014). In the tropics, grasses are the most ecologically reliable and economically justifiable feed resources (Pedreira et al., 2011) because of their morphological characteristics which enable efficient water use, and rapid recovery after periods of drought (Batistoti et al., 2012). Therefore, improving tropical forages is essential for environmental protection and sustainable livestock feed and food futures for livestock and people (Baudron et al., 2015). Brachiaria grasses are among the most important tropical grasses that originated from Africa and improved in Americas through agronomic selection and breeding (Miles et al., 2004). They have demonstrated to be highly productive, nutritive and socially acceptable in Asia and Africa for different livestock production systems (Vendramini et al., 2014). In tropical areas, the most sensitiv...

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“…The NDF content observed in most grasses within each DAP was much lower than values reported in other grasses like Lilum sp. (Fukushima et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Effect of cutting time on agronomic and nutritional characteristics of nine commercial cultivars of Brachiaria grass compared with Napier grass during establishment under semi-arid conditions in Rwanda

Mutimura¹,

Ebong²,

Rao³

et al. 2017

Afr. J. Agric. Res.

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“…Thus, altering the composition and structure of lignin has also been pursued as a means to improve digestibility. For example, plant improvement and evaluation programs often use Klason lignin (KL), acetyl bromide-soluble lignin (ABSL), or related procedures to estimate lignin content, but these methods provide differing estimates of lignin concentration and its relationship with cell wall digestibility (Fukushima and Hatfield, 2004;Gomes et al, 2011;Goff et al, 2012;Fukushima et al, 2015;van der Weijde et al, 2016). Unfortunately, efforts to relate the composition or structure of lignin to digestibility are usually confounded by concomitant changes in plant anatomy or by shifts in the concentration and distribution of lignin or other cell wall components in plant tissues (Grabber, 2005).…”

Section: Relationships Between Cell Wall Digestibility and Lignin Conmentioning

confidence: 99%

Relationships between Cell Wall Digestibility and Lignin Content as Influenced by Lignin Type and Analysis Method

Grabber

2019

Crop Science

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Difficulties in measuring the lignin content of forage and biomass crops confounds efforts by plant breeders and molecular biologists to grasp how shifts in lignin chemistry affect fiber digestibility. In this study, relationships between digestibility and lignin content were examined by artificially lignifying Zea mays L. cell walls with bulk polymers composed of p‐hydroxyphenyl‐guaiacyl (HG) and guaiacyl (G) units or with endwise polymers composed of G, guaiacyl‐syringyl (GS), and guaiacyl‐syringyl‐p‐coumaric acid (GSpCA) units. Regressions of in vitro rumen gas production or nonfermented polysaccharides (NFP) vs. lignin content accurately measured by an indirect spectrophotometric method revealed that G‐bulk and especially syringyl (S)‐rich endwise polymers were the least inhibitory to digestion. While providing biased estimates at low lignin contents, the acetyl bromide‐soluble lignin (ABSL) assay correctly ranked how endwise polymers affected the digestibility of highly lignified cell walls, but the method required unique absorption coefficients to assess relationships for bulk polymers. Conversely, the Klason lignin (KL) method provided more accurate estimates at low lignin contents, but varying recovery of KL among lignin types created bias for highly lignified cell walls. Overall, our results indicate that S‐rich lignins are the least inhibitory to digestion, but detecting desirable shifts in lignin chemistry will require the judicious use of several analytical methods.

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“…Total phenols were measured spectrophotometrically according to [35]. Cellulose and lignin were measured spectrophotometrically according to [36,37], respectively.…”

Section: Chemical Analysesmentioning

confidence: 99%

Dynamics of Organic Matter in Leaf Litter and Topsoil within an Italian Alder (Alnus cordata (Loisel.) Desf.) Ecosystem

Innangi

Danise

d’Alessandro

et al. 2017

Forests

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Abstract:Forests are the most important land ecosystems that can mitigate the earth's ongoing climate change through their ability to sequester CO 2 as C stock in forest biomass and soil. Short-rotation deciduous hardwoods or N 2 -fixing species are ideal candidates for afforestation and reforestation, given that most of the carbon accumulates in the first 30 years. Alders match both of the above-mentioned features, and Italian alder, which is less dependent on riparian habitats and more drought tolerant, is an ideal candidate. Despite this, few studies exist of this tree species and its effect on soil organic matter. In this study, we focused on litter input and leaf litter decomposition dynamics, forest floor leaf litter and topsoil (0-5 cm) organic matter, and patterns of covariation from litter to topsoil. The leaf litter was rich in nitrogen and decomposed quickly (k = 0.002 day −1 ). There was a large organic carbon stock, which varied in the leaf litter (from 1.7 ± 0.3 Mg/ha in January to 0.4 ± 0.1 Mg/ha in July) and was stable in the topsoil (on average 28.6 ± 1.5 Mg/ha). Stocks for total nitrogen, cellulose, lignin, water and ethanol extractables, and total phenols were also evaluated. In order to investigate patterns of covariation in these stocks from litter to soil, we used two-block partial least squares. The first axis showed that from January to July there was a reduction of total nitrogen, lignin and cellulose in the forest floor leaf litter, while in the topsoil there was a decrease in water extractables and total organic carbon. The second axis showed minor phenomena involving phenols, water and ethanol extractables, and total N. The fast turnover of dissolved organic matter fractions (water and ethanol extractables), linked with cellulose and lignin dynamics, might suggest that within the Italian alder ecosystem there is a reasonably fast formation of stable C compounds in the soil. Thus, Italian alder is an ideal species for afforestation and reforestation, which could be particularly interesting for land-use policies.

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Comparison of acetyl bromide lignin with acid detergent lignin and Klason lignin and correlation with in vitro forage degradability

Cited by 54 publications

References 42 publications

Effect of cutting time on agronomic and nutritional characteristics of nine commercial cultivars of Brachiaria grass compared with Napier grass during establishment under semi-arid conditions in Rwanda

Effect of cutting time on agronomic and nutritional characteristics of nine commercial cultivars of Brachiaria grass compared with Napier grass during establishment under semi-arid conditions in Rwanda

Relationships between Cell Wall Digestibility and Lignin Content as Influenced by Lignin Type and Analysis Method

Dynamics of Organic Matter in Leaf Litter and Topsoil within an Italian Alder (Alnus cordata (Loisel.) Desf.) Ecosystem

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