Comparison of ABTS, DPPH, Phosphomolybdenum Assays for Estimating Antioxidant Activity and Phenolic Compounds in Five Different Plant Extracts

Untea, A.; Lupu, Alexandra; Sărăcilă, Mihaela; Panaite, Tatiana Dumitra

doi:10.15835/buasvmcn-asb:2018.0009

Cited by 25 publications

(31 citation statements)

References 14 publications

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“…The total phenol content of HWE powder was measured spectrophotometrically according to the Folin-Ciocalteu method, as described by Untea et al (2018). The principle is to record the absorbance of a basic extract which, by complexation with the Folin-Ciocalteu reagent, absorbs in Vis domain at λ = 732 nm, wavelength.…”

Section: Methodsmentioning

confidence: 99%

Use of a hydroalcoholic extract of Salix alba L. bark powder in diets of broilers exposed to high heat stress

Sărăcilă¹,

Panaite²,

Șoica³

et al. 2019

SA J. An. Sci.

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A study was conducted to determine the effects of dietary hydroalcoholic willow bark extract powder (HWE) supplemented to broilers (14-42 days old) that were exposed to heat stress, on the performance, serum biochemical parameters, liver oxidative status and caecal microflora. The feeding trial was conducted on 120 Cobb 500 broilers (14 days old), assigned to three treatments (T0, T25, and T50), each treatment consisting of eight replicates (five chicks per replicate). The broilers were housed in an experimental hall at a 32 °C constant temperature and 23 hours light regimen. Unlike the dietary control treatment (T0), the experimental treatments were supplemented with 25 g HWE powder/100 kg diet (T25), and 50 g HWE powder 100 kg diet (T50), respectively. Dietary HWE powder did not affect the broilers' performance significantly (14-42 days). A significantly lower amount of malondialdehyde was noticed in the liver of broilers from T25 and T50 treatments in comparison with broilers from T0. Also, the serum cholesterol, triglycerides and alanine aminotransferase were significantly lower in broilers fed with T50, compared with those fed with T0. At 35 and at 42 days, the broilers from T25 and T50 recorded a significantly lower number of E. coli and staphylococci and a higher number of lactobacilli in the caecum than those of T0. It could be concluded that supplementation of dietary HWE powder reduced some of the adverse effects of heat stress, the most effective being the level of 50 g/100 kg diet.

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Section: Methodsmentioning

confidence: 99%

Use of a hydroalcoholic extract of Salix alba L. bark powder in diets of broilers exposed to high heat stress

Sărăcilă¹,

Panaite²,

Șoica³

et al. 2019

SA J. An. Sci.

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“…The free radical cation scavenging ability protocol (ABTS+) 2,2ʹ‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulphonic acid) was performed using the standard procedure(UNTEA et al., 2018). The stock solution was developed by reacting ABTS aqueous solution (7 mM) with 2,45 mM aqueous potassium persulfate solution in equal proportions, permitting them to react at room temperature in the dark for 12–16 hr.…”

Section: Methodsmentioning

confidence: 99%

“…The nanoparticles and aqueous resin extract samples were then added with the solution ABTS and incubated for 30 min. The absorbance was estimated at 734 nm wavelength and the same protocol for ascorbic acid was replicated as a standard (Sridhar & Charles, 2019; UNTEA et al., 2018).…”

Section: Methodsmentioning

confidence: 99%

In vitro anti‐inflammatory and antioxidant activities of ZnFe₂O₄and CrFe₂O₄nanoparticles synthesized usingBoswellia carteriresin

et al. 2021

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The development of plant‐based nano‐materials is considered an eco‐friendly technology because it does not involve hazardous chemicals. In this study, bimetallic ZnFe2O4 and CrFe2O4 nanoparticles were synthesized using an aqueous extract of Boswellia carteri resin. Synthesized ZnFe2O4 and CrFe2O4 nanoparticles were characterized by UV‐Vis spectroscopy, FTIR, XRD, and HR–TEM. The anti‐inflammatory activity was investigated in LPS‐stimulated RAW 264.7 macrophages, whereas antioxidant activity was examined using a Hydrogen Peroxide Scavenging Activity Assay, Nitric Oxide Scavenging Activity Assay, and ABTS Radical Scavenging Assay. ZnFe2O4 and CrFe2O4 nanoparticles demonstrated a moderate scavenger of H2O2 with IC50 values; 87.528 ± 8 μg/ml and 146.4468 ± 12 μg/ml, respectively. While they exhibited a strong scavenger of NO with IC50 values; 4.01 ± 0.7 μg/ml and 4.01 ± 0.7μg/ml, respectively. Interestingly, ZnFe2O4 and CrFe2O4 nanoparticles revealed an excellent anti‐inflammatory activity by dose‐dependently suppressing mRNA expressions of IL‐1b, IL‐6, and TNF‐α. Also, ZnFe2O4 and CrFe2O4 nanoparticles suppress the protein expression of TNF‐α. Together, our results proved that phyto‐mediated ZnFe2O4 and CrFe2O4 nanoparticles using Boswellia carteri resin have great potential in biomedical applications such as anti‐inflammatory and antioxidant. Practical applications Our phyto‐synthesized chromium iron oxide bimetallic nanoparticles (NPs) have shown a novel and potent anti‐inflammatory activity, with remarkable biosafety toward tested macrophages. Zinc iron oxide bimetallic NPs exhibited anti‐inflammatory effect with a lesser extent compared to the former, with moderate cytotoxicity against tested macrophages. Both zinc and chromium iron oxide NPs exhibited an equivalent antioxidant activity. Our resin‐capped chromium iron oxide NPs are suggested to be a competing nonsteroidal anti‐inflammatory agent; it is further recommended to establish advanced animal studies to confirm their biosafety, stability, and anti‐inflammatory activity accompanied with the antioxidant activity.

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“…The methanolic extract of plants and phytogenic mixture (n=6) were used to analyse the total polyphenols (TP) and total antioxidant capacity, following the methods described by Untea et al, (2018). The antioxidant capacity was assessed by two different assays (ABTS and DPPH).…”

Section: Antioxidant Activity Of Plant Mixturementioning

confidence: 99%

Dietary phytogenic mixture for broilers reared under thermoneutral and heat stress conditions

Sărăcilă

Dumitra

Puia

et al. 2020

Archiva Zootechnica

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During two feeding trials, the effect of dietary phytogenic mixture on the performance and oxidative stress biomarkers in the liver of broilers reared under thermoneutral conditions (TN) and heat stress (HS) was studied. A number of 60 Cobb 500 chicks/trial were sheltered in environmentally-controlled digestibility cages. On the 14 days of age, the chicks were weighted and assigned to four groups (2 groups/trial with 30 chicks/group). In the first trial, two groups (C-TN and PM-TN) were kept in thermoneutral conditions. In the second trial, other two groups were kept (C-HS and PM-HS) in heat stress (32 ±1 °C). The structure of diets was the same in both experiments. Compared with the control diet (C), the experimental diet (PM) contained the addition of 1% phytogenic mixture (bilberry leaves, peppermint leaves, fennel leaves and sea buckthorn meal). Irrespective of temperature conditions, dietary PM did not affect broiler’s performance. The dietary supplementation of PM delayed protein and lipid oxidation in the liver tissue of broilers in both trials by increasing the hepatic catalase, glutathione and superoxide dismutase activity.

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Comparison of ABTS, DPPH, Phosphomolybdenum Assays for Estimating Antioxidant Activity and Phenolic Compounds in Five Different Plant Extracts

Cited by 25 publications

References 14 publications

Use of a hydroalcoholic extract of Salix alba L. bark powder in diets of broilers exposed to high heat stress

Use of a hydroalcoholic extract of Salix alba L. bark powder in diets of broilers exposed to high heat stress

In vitro anti‐inflammatory and antioxidant activities of ZnFe₂O₄and CrFe₂O₄nanoparticles synthesized usingBoswellia carteriresin

Dietary phytogenic mixture for broilers reared under thermoneutral and heat stress conditions

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Comparison of ABTS, DPPH, Phosphomolybdenum Assays for Estimating Antioxidant Activity and Phenolic Compounds in Five Different Plant Extracts

Cited by 25 publications

References 14 publications

Use of a hydroalcoholic extract of Salix alba L. bark powder in diets of broilers exposed to high heat stress

Use of a hydroalcoholic extract of Salix alba L. bark powder in diets of broilers exposed to high heat stress

In vitro anti‐inflammatory and antioxidant activities of ZnFe2O4and CrFe2O4nanoparticles synthesized usingBoswellia carteriresin

Dietary phytogenic mixture for broilers reared under thermoneutral and heat stress conditions

Contact Info

Product

Resources

About

In vitro anti‐inflammatory and antioxidant activities of ZnFe₂O₄and CrFe₂O₄nanoparticles synthesized usingBoswellia carteriresin