Comparison of a tissue transglutaminase ELISA with the endomysium antibody test in the diagnosis of gluten-sensitive enteropathy

Abstract

Our results show that the guinea pig tTG ELISA is suitable for use as a simple diagnostic screening and follow-up method for GSE. Further studies are necessary to identify possible additional minor antigens in GSE.

The Temporal Relationship Between the Onset of Type 1 Diabetes and Celiac Disease: A Study Based on Immunoglobulin A Antitransglutaminase Screening

“…Results can be even better than those obtained from EMA detection by immunofluorescence. 29 Our results confirm this result. Nevertheless, screening for atypical CD in diabetes still attracts some controversy.…”

Calcium activation of tissue transglutaminase in radioligand binding and enzyme-linked autoantibody immunoassays in childhood celiac disease

“…Similar findings have been reported by others [9,20,21], but not by all laboratories [22]. Unfortunately, the available information about the presence of calcium in the commercial kits is limited [12].…”

A comparison of 13 guinea pig and human anti-tissue transglutaminase antibody ELISA kits

“…2 "The use of human tissue transglutaminase has been reported to be associated with fewer false negative and false positive results, and an overall performance closely comparable or equal to the "gold standard" IgA anti-endomysial antibody indirect immunofluorescence assay" Since Dieterich et al described tissue tranglutaminase (tTG), an 82-85 kDa ubiquitous enzyme, as the major autoantigen target of IgA EMA, 4 over 30 publications have appeared using this protein as the basis for an alternative assay to the IgA EMA IIF assay. Most studies used guinea pig liver tTG (gpltTG) in ELISA based assays, 5-15 18-38 but purified erythrocyte 23 and recombinant human tTG (h-tTG) 13-17 24 27 29 35 38 have also been used in ELISA, 13 23 24 27 29 38 radioimmunoassay, 14-17 35 and dot blot 27 assays. Because of its ease of use, potential for automation, objectivity in interpretation, and reduced training requirements, there is growing interest in using an ELISA based IgA anti-tTG antibody (IgA tTG) assay as an alternative to the IgA EMA IIF assay.…”