Comparison of a selective and a non-selective medium in the diagnosis of gonorrhoea to ascertain the sensitivity of Neisseria gonorrhoeae to vancomycin.

Reyn, Alice; Bentzon, Michael Weis

doi:10.1136/sti.48.5.363

Cited by 34 publications

(28 citation statements)

References 11 publications

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“…This medium was used with effect by Schmale and others (1969). Reyn and Bentzon (1972) found a similar combination to be most useful for rectal specimens-despite the fact that 8 per cent. of their plates were overgrown.…”

mentioning

confidence: 99%

Diagnosis of gonorrhoea in women. Role of the rectal sample.

Bhattacharyya¹,

Jephcott²

1974

Sexually Transmitted Infections

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Nicol (1948) demonstrated gonococci in rectal samplings of 26 of 74 consorts of men with proven gonorrhoea. In this series, five (6-7 per cent. of the total) gave positive results only in the rectum. He suggested that rectal testing should be a part of the routine examination and should always be included in the tests for cure.Jensen (1953) found positive rectal cultures in 63 of a total of 205 patients infected with gonorrhoea, an incidence of 31 per cent. In his series, four cases (2 per cent.) were found to be positive only in the rectum. He also was of the opinion that the great majority, perhaps all, of these cases of rectal gonorrhoea in women originated through peno-anal contact.

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mentioning

confidence: 99%

Diagnosis of gonorrhoea in women. Role of the rectal sample.

Bhattacharyya¹,

Jephcott²

1974

Sexually Transmitted Infections

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“…The specimens were transported in Stuart's medium to the State Serum Institute, Copenhagen, for final culture and identification. Cultures were made on a Thayer-Martin medium (Reyn and Bentzon 1972), and the final identification was made by immunofluorescence (Lind, 1975 (Kvorning, 1971). Therefore a routine examination for gonorrhoea should be performed in women who have a high risk of exposure to infection and who would not otherwise be diagnosed and treated.…”

Section: Introductionmentioning

confidence: 99%

Gonorrhoea in patients with scabies.

Nielsen¹,

Scher²,

Seier³

1976

Sexually Transmitted Infections

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“…This practice has been accompanied by an increasing prevalence of N. gonorrhoeae strains that do not grow on such media (15,28) compared with the prevalence of hypersusceptible strains before vancomycin came into use (5,21). One possible effect of hypersusceptibility to vancomycin is to reduce the probability that gonorrhea caused by these strains will be treated.…”

Section: Discussionmentioning

confidence: 99%

“…Revertants to Env' gain the resistance encoded by mtr. Differences in susceptibility to vancomycin were found in recent isolates of N. gonorrhoeae, and hypersusceptibility is recognized now as a potential source of difficulty in the laboratory diagnosis of gonorrhea (21,27). Because typical gonococcal strains are resistant to vancomycin at concentrations of .3.0 ,ug/ml (5), selective culture media containing vancomycin and several other antibacterial drugs are used in many laboratories as an aid in isolating N. gonorrhoeae strains from patients' specimens contaminated with various commensal bacteria.…”

mentioning

confidence: 99%

Vancomycin hypersusceptibility in Neisseria gonorrhoeae isolated from patients involves diverse mutations

Koelbl¹,

Catlin²

1986

Antimicrob Agents Chemother

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We investigated the genetic determinants of hypersusceptibility to vancomycin and erythromycin found in Neisseria gonorrhoeae strains isolated from patients. In terms of resistance (highest concentration of antibiotic permitting growth), the levels of vancomycin resistance of six strains ranged from 0.2 to 1.0 ,ug/ml, and the level of erythromycin resistance of these strains was 0.02 or 0.05 ,ug/ml. DNA from these strains was used to introduce their hypersusceptibility determinants into partially isogenic derivatives of N. gonorrhoeae 89 which initially had wild-type levels of resistance to vancomycin (.3.0 ,ug/ml) and erythromycin (.0.1 ,ug/ml). The recombination frequencies found in reciprocal transformation tests of six isogenic strains indicated that the mutations responsible for vancomycin hypersusceptibility were located at different sites. The transformants selected for increased resistance to vancomycin were also resistant to erythromycin. This evidence, together with DNA concentration-response curves, indicated that the mutations affected either one gene locus or closely linked loci. The recombination indices obtained in crosses between our hypersusceptible strains and DNAs from reference strains carrying the envelope mutations env-l, env-2, env-3, and env-10 showed that the mutation (designated env-12) responsible for erythromycin hypersusceptibility in one strain (89-954) was located in close proximity to env-2. The determinant of vancomycin hypersusceptibiity in strain 89-954 was distinct from env-12, but the two were linked. In the other five isogenic strains, the hypersusceptibilities to both vancomycin and erythromycin could be annulled by spontaneous mutations in a locus provisionally designated vel because of its likely effects on the envelope. Vel+ mutants obtained by selection with either vancomycin alone or erythromycin alone gained increased resistance to both antibiotics.

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Comparison of a selective and a non-selective medium in the diagnosis of gonorrhoea to ascertain the sensitivity of Neisseria gonorrhoeae to vancomycin.

Cited by 34 publications

References 11 publications

Diagnosis of gonorrhoea in women. Role of the rectal sample.

Diagnosis of gonorrhoea in women. Role of the rectal sample.

Gonorrhoea in patients with scabies.

Vancomycin hypersusceptibility in Neisseria gonorrhoeae isolated from patients involves diverse mutations

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